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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2017, Vol. 11 Issue (1) : 5
Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant hydrolysate
Jiuxiao Hao,Xiujin Wang,Hui Wang()
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China
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Valerate-hydrolysate enriched culture showed great ability to produce 3HV and 3H2MV.

Valerate-hydrolysate enriched culture had more Brevundimonas in the community.

Mixed iso-/n-valerate was effective at balancing microbial growth and PHAs synthesis.

Co-substrates of valerate and propionate raised the fraction of 3HV and 3H2MV.

The production of polyhydroxyalkanoates (PHAs) with a high fraction of 3-hydroxyvalerate (3HV) and 3-hydroxy-2-methylvalerate (3H2MV) from mixed culture enriched by valerate-dominant hydrolysate was evaluated in this study. After long-term enrichment, the culture showed strong ability to synthesize 3HV and 3H2MV, even with acetate-dominant substrate. The ultilization of single or mixed iso-/n-valerate by the enriched culture showed that the mixture of iso-valerate and n-valerate was more efficient substrate than any single in terms of balancing microbial growth and PHAs synthesis. Besides, through comparing the kinetics and stoichiometry of the tests supplying valerate and propionate, the enriched culture with equivalent valerate and propionate (1:1 molar ratio) exhibited superior PHAs production performances to pure valerate or propionate, attaining more than 70 mol% of 3HV and 3H2MV. The above findings reveal that valerate-dominant hydrolysate is a kind of suitable substrate to enrich PHAs producing culture with great capability to synthesize 3HV and 3H2MV monomers, thus improving product properties than pure poly(3-hydroxybutyrate) (P3HB); also 3HV and 3H2MV production behaviors can be regulated by the type of odd-carbon VFAs in the substrate.

Keywords Polyhydroxyalkanoates (PHAs)      Valerate      Mixed culture      3-hydroxyvalerate (3HV)      Propionate     
Corresponding Authors: Hui Wang   
Issue Date: 23 December 2016
 Cite this article:   
Jiuxiao Hao,Xiujin Wang,Hui Wang. Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant hydrolysate[J]. Front. Environ. Sci. Eng., 2017, 11(1): 5.
VFAs/(C mmol·L1) biomass enrichment batch tests a)
valerate-dominant hydrolysate
acetate-dominant hydrolysate b)
single iso-Val
single n-Val
mixed iso-/n-Val c)
pure Val c)
pure Pro
Val-Pro 1:1
total 100 100 100 100 100 100 100 100
acetate 25 50 0 0 0 0 0 0
propionate 12.5 25 0 0 0 0 100 50
butyrate 12.5 25 0 0 0 0 0 0
iso-valerate 25 0 100 0 50 50 0 25
n-valerate 25 0 0 100 50 50 0 25
Tab.1  Composition of VFAs in different substrates for PHAs culture enrichment and batch experiments
Fig.1  Cyclic profiles of (a) substrate utilization and PHAs production in two SBRs, and the usage of individual VFA in (b) valerate-hydrolysate SBR and (c) acetate-hydrolysate SBR (“Valerate” includes iso-valerate and n-valerate, bars= S.D., n = 3)
Fig.2  Concentrations of CDW, PHAs and active biomass (X) during one cycle in (a) valerate-hydrolysate SBR and (b) acetate-hydrolysate SBR, and (c) compositions of synthesized PHAs (The red and blue dashed lines mark the end of feast phase, bars= S.D., n = 3)
parameter valerate-hydrolysate
enriched culture
enriched culture
species richness species richness
Phylum level a) Proteobacteria 85.25% Proteobacteria 71.00%
Bacteroidetes 14.51% Bacteroidetes 28.64%
Genus level a) Brevundimonas 80.90% Brevundimonas 63.81%
Sphingobacterium 14.05% Sphingobacterium 27.78%
Paenochrobactrum 1.42%
Devosia 1.31%
Pseudaminobacter 1.03%
Chao 1 richness b) 297.38 384.77
ACE richness b) 334.82 399.15
Tab.2  Relative taxonomic abundance at phylum and genus levels for valerate-hydrolysate enriched culture and acetate-hydrolysate enriched culture, and their species richness
Fig.3  (a) Substrate utilization, (b) PHAs production and (c) PHAs composition from batch test by valerate-hydrolysate enriched culture with acetate-hydrolysate (bars= S.D., n = 3)
Fig.4  PHAs production behaviors of valerate-hydrolysate enriched culture with (a) iso-valerate, (b) n-valerate, and (c) mixed iso-/n-valerate, as well as (d) their PHAs compositions at the moment with maximum PHAs concentration (bars= S.D., n = 3)
substrate b) qValc) /(C mmol·(L·h)1) qProc) /(C mmol·(L·h)1) PHAmax /(g·L1) Xmax /(g·L1) YPHA/Sd) /(C mmol per C mmol) YX/Sd) /(C mmol per C mmol) 3HB/3HV/3H2MV /mol% qPHAd) /(g·(L·h)1) q3HV24h /(g·(L·h)1) q3H2MV24h /(g·(L·h)1)
24h 36h
pure valerate 1.63 30 h: 0.54 48 h: 0.82 0.45 0.3 65.21/25.84/8.95 53.26/35.10/11.64 0.018 0.0058 0.0022
pure propionate 2.27 18 h: 0.36 18 h: 1.48 0.38 1.45 66.31/19.29/14.40 62.84/18.13/19.03 0.02 0.0026 0.0021
valerate-propionate 1:1 1.41 1.32 30 h: 0.95 12 h: 0.95 0.48 0.74 28.42/67.27/9.31 27.15/68.51/4.34 0.032 0.0246 0.0038
Tab.3  Kinetics and stoichiometry of batch PHAs production tests from valerate-hydrolysate enriched culture with valerate and propionate as substrate a)
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