Transformation of triclosan by a novel cold-adapted laccase from <i>Botrytis</i>sp. FQ

doi:10.1007/s11783-017-0927-5

Front. Environ. Sci. Eng.

2017, Vol. 11

Issue (3) : 6 https://doi.org/10.1007/s11783-017-0927-5

RESEARCH ARTICLE

Transformation of triclosan by a novel cold-adapted laccase from Botrytissp. FQ

Yuanyuan Shi¹, Deyang Kong², Jiayang Liu³(

), Junhe Lu¹(

), Xiaoming Yin¹, Quansuo Zhou¹

¹. College of Resources and Environmental Science, Nanjing Agricultural University, Nanjing 210095, China
². Nanjing Institute of Environmental Science, Ministry of Environmental Protection of PRC, Nanjing 210042, China
³. School of Bioengineering, Huanghuai University, Zhumadian 463000, China

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Abstract

A cold-adapt laccase excreted by a fungi from rotten tomato was characterized.

The laccase can effectively transform triclosan to form polymerized products.

The reaction rate is first order to the concentrations of both laccase and triclosan.

The reaction was inhibited by humic acid.

This work investigated the transformation of triclosan (TCS) by laccase produced by a pathogen isolated from rotten tomato. The pathogen was characterized asBotrytis sp. FQ, belonging to subphylum Deuteromycotina. The laccase exhibited cold-adaptation with relatively high activity at 20°C. The laccase could effectively transform TCS. Approximately 62% TCS could be removed at dose of 1.0 unit·mL⁻¹ in 120 min. The reaction rate appeared to be pseudo-first-order to the concentration of the substrate, suggesting the laccase activity remained stable during the reaction. Transformation products of TCS were analyzed by mass spectrometry and it was revealed that TCS dimers were formed via radical coupling pathways. During this process, laccase catalyzed oxidation of TCS to form a radical intermediate is the rate limiting step. However, this step can be reversed by humic acid. Overall, the laccase showed great potential in the treatment of phenolic contaminants. Since laccase is widely presented in natural environment, this study also revealed an important pathway involved in the transformation of phenolic contaminants in the environment.

Keywords Laccase Botrytis cinerea Triclosan Transformation Kinetics

Corresponding Author(s): Jiayang Liu,Junhe Lu

Issue Date: 13 April 2017

Cite this article:

Yuanyuan Shi,Deyang Kong,Jiayang Liu, et al. Transformation of triclosan by a novel cold-adapted laccase from Botrytissp. FQ[J]. Front. Environ. Sci. Eng., 2017, 11(3): 6.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-017-0927-5
https://academic.hep.com.cn/fese/EN/Y2017/V11/I3/6

Fig.1 (a) Top View of the fungus growing on PDA plate for 7 days at 4℃. (b) Decolorization of RBBR by the fungus on PDA plate for 7 days’ cultivation at 4℃. (c) Conidium of the fungus by SEM (1500 ×). (d) Conidiophore of the fungus by SEM (1200 ×)

Fig.2 Laccase production by Botrytis sp. FQ via liquid fermentation at varying temperatures.

Fig.3 Effects of pH (a) and temperature (b) on activity of crude laccase by Botrytis sp. FQ.

Fig.4 Kinetic plot of TCS degradation at varying laccase dose. Insert is the relationship of k_obs versus laccase dose. Experimental conditions: [TCS]₀ = 3.5 mmol·L^-¹, T = 20℃, pH= 6.8

Fig.5 MS of TCS transformation products by laccase: [TCS]₀ = 3.5 mmol·L^-¹, [laccase]₀ = 1.0 unit·mL^-¹, T = 20°C, pH= 6.8, V = 100 mL, and reaction time= 120 min

Fig.6 Kinetic plot of TCS degradation in laccase catalytic process in the presence of HA. Insert is the relationship betweenk_obs and HA concentration. Experimental conditions: [TCS]₀ = 3.5 mmol·L^-¹, [laccase]₀ = 1.0 unit·mL^-¹

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