Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and a traditional SBR treating digested piggery wastewater

doi:10.1007/s11783-017-0929-3

Front. Environ. Sci. Eng.

2017, Vol. 11

Issue (3) : 8 https://doi.org/10.1007/s11783-017-0929-3

RESEARCH ARTICLE

Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and a traditional SBR treating digested piggery wastewater

Xiaolin Sheng¹, Rui Liu¹(

), Xiaoyan Song¹, Lujun Chen^1,²(

), Kawagishi Tomoki³

¹. Zhejiang Provincial Key Laboratory of Water Science and Technology, Department of Environment in Yangtze Delta Region Institute of Tsinghua University, Zhejiang, Jiaxing 314006, China
². School of Environment, Tsinghua University, Beijing 100084, China
³. Aqua Development Center, Mitsubishi Rayon Co. Ltd., Toyohashi 4408601, Japan

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Abstract

IASBRs achieved a higher level of TN and NH₄⁺-N removals than the SBR.

IASBRs had higher abundance of denitrification–related bacteria than the SBR.

The denitrifiers abundance was correlated with the TN removal rate.

The NH₄⁺–N removal rate might relate to the AOB activity.

A traditional sequencing batch reactor (SBR) and two intermittently aerated sequencing batch reactors (IASBRs) were parallelly operated for treating digested piggery wastewater. Their microbial communities were analyzed, and the nitrogen removal performance was compared during the long–term run. IASBRs demonstrated higher removal rates of total nitrogen (TN) and ammonium nitrogen (NH₄⁺-N) than the SBR, and also demonstrated higher resistance against TN shock load. It was found that the more switch times between aerobic/anoxic in an IASBR, the higher the removal rates of TN and NH₄⁺–N. All the reactors were predominated by Thauera, Nitrosomonas and Nitrobacter, which were considered to be species of denitrifiers, ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB), respectively. However, the abundance and diversity was of great difference. Compared with SBR, IASBRs achieved higher abundance of denitrification–related bacteria. IASBR 1 with four aerobic/anoxic switch times was detected with 25.63% of Thauera, higher than that in IASBR 2 with two aerobic/anoxic switch times (11.57% of Thauera), and much higher than that in the SBR (only 6.19% of Thauera). IASBR 2 had the highest percentage of AOB, while IASBR 1 had the lowest percentage. The denitrifiers abundance was significantly positive correlated with the TN removal rate. However, the NH₄⁺–N removal rate showed no significant correlation with the AOB abundance, but might relate to the AOB activity which was influenced by the average free ammonium (FA) concentration. Nitrobacter was the only NOB genus detectable in all reactors, and were less than 0.03%.

Keywords Digested piggery wastewater Intermittent aeration Microbial community Partial nitrification–denitrification process Sequencing batch reactor (SBR)

Corresponding Author(s): Rui Liu,Lujun Chen

Issue Date: 19 April 2017

Cite this article:

Xiaolin Sheng,Rui Liu,Xiaoyan Song, et al. Comparative study on microbial community in intermittently aerated sequencing batch reactors (SBR) and a traditional SBR treating digested piggery wastewater[J]. Front. Environ. Sci. Eng., 2017, 11(3): 8.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-017-0929-3
https://academic.hep.com.cn/fese/EN/Y2017/V11/I3/8

Tab.1 Operational conditions in the three reactors

Fig.1 Profile of NH₄⁺–N, TN, NO₂^––N and COD in three reactors

Fig.2 Profiles of FA in a typical operational cycle of the three reactors

Tab.2 Diversity of microbial community in the three reactors

Fig.3 Bacterial community structure at phylum level (a), the classes in Proteobacteria (b) and evolution of bacterial community at genus level (c)

Fig.4 Bacterial community structure at genus level in IASBR 1^# (a), IASBR 2^# (b) and SBR (c)

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