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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

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Front. Environ. Sci. Eng.    2017, Vol. 11 Issue (4) : 6    https://doi.org/10.1007/s11783-017-0932-8
RESEARCH ARTICLE |
A full-scale integrated-bioreactor with two zones treating odours from sludge thickening tank and dewatering house: performance and microbial characteristics
Jianwei Liu1,2, Kaixiong Yang2,3, Lin Li2,3(), Jingying Zhang2,3
1. Key Laboratory of Urban Stormwater System and Water Environment, Beijing University of Civil Engineering and Architecture, Ministry of Education, Beijing 100044, China
2. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
3. University of Chinese Academy of Sciences, Beijing 100049, China
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Abstract

The integrated-bioreactor consists of a suspended zone and an immobilized zone.

H2S and NH3 from WWTP were effectively eliminated by the integrated-bioreactor.

Different microbial populations dominated in the individual zones.

Most of the H2S was bio-oxidized into elemental sulfur and sulfate in IZ.

Large amount of NH3 was converted into nitrate and nitrite in SZ.

A full-scale integrated-bioreactor consisting of a suspended zone and an immobilized zone was employed to treat the ordours emitted from a wastewater treatment plant. The inlet concentrations of H2S and NH3 were 1.6–38.6 mg·m3 and 0.1–6.7 mg·m3, respectively, while the steady-state outlet concentrations were reduced to 0–2.8 mg·m3 for H2S and 0–0.5 mg·m3 for NH3. Both H2S and NH3 were eliminated effectively by the integrated-bioreactor. The removal efficiencies of H2S and NH3 differed between the two zones. Four species of microorganisms related to the degradation of H2S and NH3 were isolated. The characteristics and distributions of the microbes in the bioreactor depended on the inlet concentration of substrates and the micro-environmental conditions in the individual zones. Product analysis indicated that most of the H2S was oxidized into sulfate in the immobilized zone but was dissolved into the liquid phase in the suspended zone. A large amount of NH3 was converted into nitrate and nitrite by nitration in the suspended zone, whereas only a small amount of NH3 was transferred to the aqueous phase mainly by absorption or chemical neutralization in the immobilized zone. Different microbial populations dominated the individual zones, and the major biodegradation products varied accordingly.

Keywords Biological deodorization      Microbial characteristics      Ammonia      Hydrogen sulfide      Wastewater treatment plant     
Corresponding Authors: Lin Li   
Issue Date: 26 April 2017
 Cite this article:   
Jianwei Liu,Kaixiong Yang,Lin Li, et al. A full-scale integrated-bioreactor with two zones treating odours from sludge thickening tank and dewatering house: performance and microbial characteristics[J]. Front. Environ. Sci. Eng., 2017, 11(4): 6.
 URL:  
http://academic.hep.com.cn/fese/EN/10.1007/s11783-017-0932-8
http://academic.hep.com.cn/fese/EN/Y2017/V11/I4/6
Fig.1  (a) Schematic diagram of the treatment process: (1) the suspended zone, (2) the immobilized zone, (3) air pump, (4) flowmeter, (5) sampling port 1, (6) monitor instrument 1, (7) monitor instrument 2, (8) sampling port 2, (9) sampling port 3, (10) sampling port 4, (11) meter pump, (12) the nutrient solution tank; (b) the integrated-bioreactor for odour control
compoundsmolecularOTCs a)
/ppm
Hib) /(25℃)concentrations /(mg·m3)
ST c)SD d)
hydrogen sulfideH2S0.180.920.1–7.81.5–38.8
ammoniaNH30.900.0050.1–3.21.0–6.5
methyl mercaptanCH4S0.10 × 103IS f)0–0.0010–0.001
methyl sulfideC2H6SU e)ISND g)0–0.001
styreneC8H80.15–25IS0.01–0.050.01–0.10
Tab.1   Main composition of odors
Fig.2  Inlet and outlet concentration and removal efficiency for H2S. Cin: inlet concentration; Cout: outlet concentration; Re: removal efficiency
Fig.3  Inlet and outlet concentration and removal efficiency for NH3. Cin: inlet concentration; Cout: outlet concentration; Re: removal efficiency
speciesthe suspended zonethe immobilized zone
acidophilic Thiobacilli9.50±1.01 × 105 CFU a)·mL11.32±0.32 × 107 CFU·g1
non-acidophilic Thiobacilli9.85±0.99 × 106 CFU·mL12.13±0.37 × 106 CFU·g1
ammonia oxidizing bacteria1.70±0.26 × 104 MPN b)·mL16.97±0.95 × 102 MPN·g1
nitrite oxidizing bacteria5.60±0.87 × 104 MPN·mL14.59±0.44 × 103 MPN·g1
Tab.2  Microorganisms in the integrated-bioreactor at steady-state
Fig.4  Changes of pH in the suspended zone and the immobilized zone
time /dnon-acidophilic Thiobacilli
/(CFU a)·mL1)
acidophilic Thiobacilli /(CFU·mL1)SO42
/(mg·L1)
S2
/(mg·L1)
suspended zone
101.9±0.39 × 1049.5±1.85 × 1030.15±0.024.46±0.11
351.5±0.36 × 1045.44±0.69 × 1040.61±0.135.01±0.13
105not detectednot detected0.82±0.034.96±0.20
1559.85±0.99 × 1069.5±1.01 × 1051.00±0.184.15±0.13
immobilized zone
104.57±0.61 × 1051.32±0.14 × 1040.18±0.013.06±0.12
358.6±0.82 × 1054.17±0.35 × 1061.54±0.101.23±0.19
105not detectednot detected3.71±0.080.24±0.05
1552.13±0.37 × 1061.32±0.32 × 1072.55±0.070.28±0.03
Tab.3  Changes of Thiobacilli and products in H2S removal
Fig.5  Percentage of various nitrogen containing compounds in individual zone
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