Preparation and characterization of a novel microorganism embedding material for simultaneous nitrification and denitrification

doi:10.1007/s11783-017-0961-3

Front. Environ. Sci. Eng.

2017, Vol. 11

Issue (6) : 15 https://doi.org/10.1007/s11783-017-0961-3

RESEARCH ARTICLE

Preparation and characterization of a novel microorganism embedding material for simultaneous nitrification and denitrification

Ming Zeng¹, Ping Li¹, Nan Wu², Xiaofang Li¹, Chang Wang¹(

)

¹. College of Marine and Environmental Science, Tianjin University of Science &Technology, Tianjin 300457, China
². College of Engineering and Technology, Tianjin Agricultural University, Tianjin 300384, China

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Abstract

CD enhances the hydrophilic property of traditional PVA-SA gel solution.

CD increases the density of embedded microorganism and micro porosity of bead.

CD makes the maximum endogenous respiration rate being high.

30-1.7-CD contributes the highest total inorganic nitrogen removal efficiency.

Comamonas sp. mainly realize the simultaneous nitrification and denitrification.

A novel microorganism embedding material was prepared to enhance the biological nitrogen removal through simultaneous nitrification and denitrification. Polyvinyl alcohol (PVA), sodium alginate (SA) and cyclodextrin (CD) were used to compose gel bead with embedded activated sludge. The effects of temperature, CD addition and concentrations of PVA and SA on nitrogen removal were evaluated. Results show that the gel bead with CD addition at 30°C contributed to the highest nitrogen removal efficiency and nitrogen removal rate of 85.4% and 2.08 mg·(L·h)?1, respectively. Meanwhile, negligible NO3? and NO2? were observed, proving the occurrence of simultaneous nitrification and denitrification. The High-Throughput Sequencing confirms that the microbial community mainly contained Comamonadaceae in the proportion of 61.3%. Overall, CD increased gel bead’s porosity and resulted in the high specific endogenous respiration rate and high nitrogen removal efficiency, which is a favorable additional agent to the traditional embedding material.

Keywords Immobilization technology Nitrogen removal Cyclodextrin Microbial community Wastewater treatment

Corresponding Author(s): Chang Wang

Issue Date: 23 June 2017

Cite this article:

Ming Zeng,Ping Li,Nan Wu, et al. Preparation and characterization of a novel microorganism embedding material for simultaneous nitrification and denitrification[J]. Front. Environ. Sci. Eng., 2017, 11(6): 15.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-017-0961-3
https://academic.hep.com.cn/fese/EN/Y2017/V11/I6/15

Tab.1 Summary of physical properties of different gel beads

Fig.1 Adsorption curves of methylene blue for gel beads with and without CD

Fig.2 Fourier transforms infrared spectra of different compounds

Fig.3 The SEM micrograph of gel bead prepared by PVA and SA concentrations of both 1.2% with embedding microorganisms (A: external of bead with CD; B: internal of bead with CD; C: external of bead without CD; D: internal of bead without CD)

Tab.2 Respiration rates of gel beads with embedded microorganism of four treatments and suspended sludge

Fig.4 The comparison of (A) TIN removal efficiencies and (B) ammonium concentrations of four treatments during the batch test (30-1.7-CD: 1.7% PVA+ 1.7% SA+ 1.0% CD at 30°C; 30-1.2: 1.2% PVA+ 1.2% SA without CD at 30°C; 25-1.2-CD: 1.2% PVA+ 1.2% SA+ 1.0% CD at 25°C; 25-1.7: 1.7% PVA+ 1.7% SA without CD at 25°C)

Tab.3 Results of orthogonal experiments

Fig.5 The barplot of relative abundance of mcrobial communities inside gel bead

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