Detection of oxidative stress and DNA damage in freshwater snail <i>Lymnea leuteola</i> exposed to profenofos

doi:10.1007/s11783-018-1039-6

Front. Environ. Sci. Eng.

2018, Vol. 12

Issue (5) : 1 https://doi.org/10.1007/s11783-018-1039-6

RESEARCH ARTICLE

Detection of oxidative stress and DNA damage in freshwater snail Lymnea leuteola exposed to profenofos

Daoud Ali¹(

), Huma Ali², Saud Alifiri¹, Saad Alkahtani¹, Abdullah A Alkahtane¹, Shaik Althaf Huasain¹

¹. Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
². Department of Chemistry, MANIT, Bhopal 462003, India

Download: PDF(802 KB) HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks

Abstract

Freshwater snail (Lymnea luteola L.) is good bio indicator of water pollution.

Profenofos is tested for its molluscicidal activity against Lymnea luteola L. snail.

Deleterious effects on some oxidative stress were detected.

Profenofos has a genotoxic effect on Lymnea luteola L. snails.

Extensive production and use of organophosphate pesticide in agriculture, has risen concerned about its ecotoxicity and risk assessment of insecticides, which are more important. Therefore, the present investigation was aimed to study the induction of oxidative stress and DNA damage by organophosphate insecticide profenofos (PFF) in freshwater snail Lymnea luteola (L. luteola). The median lethal value (96 h LC₅₀) of PFF was estimated as 1.26 mg/L for L. luteola in a semi-static system and on the basis of LC₅₀ value three concentrations viz., 0.126 (1/10 of LC_50, Sublethal I), 0.63 (1/2 of LC_50, Sublethal II) and 0.84 mg/L (2/3 of LC_50, Sublethal III) were determined. Snails were exposed to above-mentioned concentrations of PFF along with solvent control (acetone) and negative control for 96 h. The haemolymph was collected at 24 and 96 h of after treatment. In heamolymph of PFF exposed snail, lipid peroxide, glutathione reduced glutathione S transferase and superoxide dismutase activities at the tested concentrations significantly differ from those in the control. The genotoxicity induced in hemocytes of treated snails was measured by alkaline single cell gel electrophoresis assay. The data of this experiment demonstrated significantly enhancement of oxidative stress and DNA damage in the treated snails as compared to controls. Also, we observed statistically significant correlations of ROS with DNA damage (% tail DNA) (R² = 0.9708) for 24 h and DNA damage (R² = 0.9665) for 96 h.

Results of the current experiment can be useful in risk assessment of PFF among aquatic organisms. The study confirmed the use of comet assay for in vivo laboratory experiments using freshwater snail for selecting the toxic potential of industrial chemicals and environmental contaminants.

HIGHLIGHTS

GRAPHIC ABSTRACT

Keywords Acute toxicity Profenofos ROS oxidative stress DNA damage Lymnea luteola

Corresponding Author(s): Daoud Ali

Issue Date: 18 August 2018

Cite this article:

Daoud Ali,Huma Ali,Saud Alifiri, et al. Detection of oxidative stress and DNA damage in freshwater snail Lymnea leuteola exposed to profenofos[J]. Front. Environ. Sci. Eng., 2018, 12(5): 1.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-018-1039-6
https://academic.hep.com.cn/fese/EN/Y2018/V12/I5/1

Fig.1 Changes in the total number of circulating haemocyte (mm³) in the control and PFF exposed L. luteola. Each value represents the mean±S.E. of three observations in duplicate. *p<0.05, **p<0.01 vs. control

Fig.2 Profenofos induced ROS generation in heamocyte cells (A). Images of qualitative analysis by using phase contrast cum fluorescence microscope (Nikon, model 80i) (B). ROS generation (%) in heamocyte cells. Each value represents the mean ?S.E. of three experiments. *p<0.05, **p<0.01 vs. control

Fig.3 (A) Levels of LPO (B). GSH (C). SOD (D). GST in heamocyte cells after exposure to PFF for 24 and 96 h. Each value represents the mean±S.E. of three experiments. *p<0.05, **p<0.01 vs. control

Fig.4 DNA fragments in heamocyte cells due to PFF A. Tail DNA (%) B. Olive tail moment C. Correlation between ROS generation and DNA fragments (% tail DNA) in 24 h D. Correlation between ROS generation and DNA fragments (% tail DNA) in 96 h. Each value represents the mean±S.E. of three experiments. *p<0.05, **p<0.01 vs. control

1	Akcha F, Vincent Hubert F, Pfhol-Leszkowicz A (2003). Potential value of the comet assay and DNA adduct measurement in dab (Limanda limanda) for assessment of in situ exposure to genotoxic compounds. Mutat Res, 534(1-2): 21–32 https://doi.org/10.1016/S1383-5718(02)00244-9 pmid: 12504752
2	Ali D, Ahmed M, Alarifi S, Ali H (2015). Ecotoxicity of single-wall carbon nanotubes to freshwater snail Lymnaea luteola L.: Impacts on oxidative stress and genotoxicity. Environmental Toxicology, 30(6): 674–682 https://doi.org/10.1002/tox.21945 pmid: 24449093
3	Ali D, Nagpure N S, Kumar S, Kumar R, Kushwaha B, Lakra W S (2009). Assessment of genotoxic and mutagenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food and Chemical Toxicology, 47(3): 650–656 https://doi.org/10.1016/j.fct.2008.12.021 pmid: 19141310
4	Anderson D, Yu T W, Phillips B J, Schmezer P (1994). The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutation Research, 307(1): 261–271 https://doi.org/10.1016/0027-5107(94)90300-X pmid: 7513806
5	APHA AWWA, WPCF (1998). Standard methods for examination of water and wastewater, 20th ed. American Public Health Association, New York
6	Arabi M, Alaeddini M A (2005). Metal ion mediated oxidative stress in the gill homogenate of rainbow trout (Onchorhynchus mykiss). Biological Trace Element Research, 108: 155–168 https://doi.org/10.1385/BTER:108:1-3:155 pmid: 16327069
7	Becker J S, Füllner K, Seeling U D, Fornalczyk G, Kuhn A J (2008). Measuring magnesium, calcium and potassium isotope ratios using ICP-QMS with an octopole collision cell in tracer studies of nutrient uptake and translocation in plants. Analytical Bioanalytical Chemistry, 390(2): 571–578 https://doi.org/10.1007/s00216-007-1603-6 pmid: 17962924
8	Demir F, Uzun F G, Durakn D, Kalender Y (2011). Subacute chlorpyrifos induced oxidative stress in rat erythrocytes and the protective effects of catechin and quercetin. Pesticide Biochemical Physiology, 99(1): 79–81 https://doi.org/10.1016/j.pestbp.2010.11.002
9	Finney D J (1971). Probit Analysis. Cambridge: Cambridge University Press, 333 p
10	Köhler H R, Triebskorn R (2013). Wildlife ecotoxicology of pesticides: Can we track effects to the population level and beyond? Science, 341(6147): 759–765 https://doi.org/10.1126/science.1237591 pmid: 23950533
11	Kohn K W (1991). Principles and practice of DNA filter elution. Pharmacology & Therapeutics, 49(1-2): 55–77 https://doi.org/10.1016/0163-7258(91)90022-E pmid: 1852788
12	Kumaravel T S, Jha A N (2006). Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutation Research, 605(1-2): 7–16 https://doi.org/10.1016/j.mrgentox.2006.03.002 pmid: 16621680
13	Lever J, Bekius R (1965). On the presence of an external hemal pore in Lymnaea stagnalis L. Experientia, 21(7): 395–396 https://doi.org/10.1007/BF02139763 pmid: 5870770
14	Liu Y, Wang J, Wei Y, Zhang H, Xu M, Dai J (2008). Induction of time-dependent oxidative stress and related transcriptional effects of perfluorododecanoic acid in zebrafish liver. Aquatic Toxicology, 89(4): 242–250 https://doi.org/10.1016/j.aquatox.2008.07.009 pmid: 18760846
15	Lushchak V I (2011). Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicology, 101(1): 13–30 https://doi.org/10.1016/j.aquatox.2010.10.006 pmid: 21074869
16	Malla T M, Senthikumar C S, Akhtar S, Ganesh N (2011). Micronuclei as an evidence of DNA damage in fresh water catfish Heteroneustes fossilis (Blotch) exposed to synthetic sindoor, ARPN Journal of Agricultural and Biological Science, 95: 351–358
17	Miller G T (2004). Chapter 9, Sustaining the Earth, 6th ed. Pacific Grove, California: Thompson Learning, Inc., 211–216
18	Monteiro D A, de Almeida J A, Rantin F T, Kalinin A L (2006). Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology—Part C, 143(2): 141–149 pmid: 16546452
19	Ohkawa H, Ohishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95(2): 351–358 https://doi.org/10.1016/0003-2697(79)90738-3 pmid: 36810
20	Palmer W E, Bromley P T, Brandenburg R L (2007). Wildlife and pesticides- peanuts. North Carolina Cooperative Extension Service
21	Pamanji R, Yashwanth B, Bethu M S, Leelavathi S, Ravinder K, Rao J V (2015). Toxicity effects of profenofos on embryonic and larval development of Zebrafish (Danio rerio). Environmental Toxicology Pharmacol, 39(2): 887–897 https://doi.org/10.1016/j.etap.2015.02.020 pmid: 25796049
22	Rank J, Jensen K, Jespersen PH (2005). Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark Mutation Research, 585: 33–42
23	Rowe L A, Degtyareva N, Doetsch P W (2008). DNA damage-induced reactive oxygen species (ROS) stress response in Saccharomyces cerevisiae. Free Radical Biology and Medicine, 45(8): 1167–1177 https://doi.org/10.1016/j.freeradbiomed.2008.07.018 pmid: 18708137
24	Subudhi A W, Davis S L, Kipp R W, Askew E W (2001). Antioxidant status and oxidative stress in elite alpine ski racers. International Journal of Sport Nutrition and Exercise Metabolism, 11(1): 32–41 https://doi.org/10.1123/ijsnem.11.1.32 pmid: 11334023
25	Valencia A, Kochevar I E (2006). Ultraviolet A induces apoptosis via reactive oxygen species in a model for Smith-Lemli-Opitz syndrome. Free Radical Biology and Medicine, 40(4): 641–650 https://doi.org/10.1016/j.freeradbiomed.2005.09.036 pmid: 16458195
26	Yassi A, Kjellstrom T (1997). Linkages between environmental and occupational health. Chapter 53 environmental health hazards PartVII-The environment. Encyclopaedia of occupational health and safety, 4th ed.

[1]	Yi Qian, Weichuan Qiao, Yunhao Zhang. *Toxic effect of sodium perfluorononyloxy-benzenesulfonate on Pseudomonas stutzeri* in aerobic denitrification, cell structure and gene expression**[J]. Front. Environ. Sci. Eng., 2021, 15(5): 100-.
[2]	Guanyu Jiang, Can Wang, Lu Song, Xing Wang, Yangyang Zhou, Chunnan Fei, He Liu. Aerosol transmission, an indispensable route of COVID-19 spread: case study of a department-store cluster[J]. Front. Environ. Sci. Eng., 2021, 15(3): 46-.
[3]	Fan Lu, Tianyu Hu, Shunyan Wei, Liming Shao, Pinjing He. Bioaerosolization behavior along sewage sludge biostabilization[J]. Front. Environ. Sci. Eng., 2021, 15(3): 45-.
[4]	Wenwen Xie, Yanpeng Li, Wenyan Bai, Junli Hou, Tianfeng Ma, Xuelin Zeng, Liyuan Zhang, Taicheng An. The source and transport of bioaerosols in the air: A review[J]. Front. Environ. Sci. Eng., 2021, 15(3): 44-.
[5]	Mariana Valdez-Castillo, Sonia Arriaga. Response of bioaerosol cells to photocatalytic inactivation with ZnO and TiO₂ impregnated onto Perlite and Poraver carriers[J]. Front. Environ. Sci. Eng., 2021, 15(3): 43-.
[6]	Pil Uthaug Rasmussen, Katrine Uhrbrand, Mette Damkjær Bartels, Helle Neustrup, Dorina Gabriela Karottki, Ute Bültmann, Anne Mette Madsen. Occupational risk of exposure to methicillin-resistant Staphylococcus aureus (MRSA) and the quality of infection hygiene in nursing homes[J]. Front. Environ. Sci. Eng., 2021, 15(3): 41-.
[7]	Philippa Douglas, Daniela Fecht, Deborah Jarvis. Characterising populations living close to intensive farming and composting facilities in England[J]. Front. Environ. Sci. Eng., 2021, 15(3): 40-.
[8]	Yunping Han, Lin Li, Ying Wang, Jiawei Ma, Pengyu Li, Chao Han, Junxin Liu. Composition, dispersion, and health risks of bioaerosols in wastewater treatment plants: A review[J]. Front. Environ. Sci. Eng., 2021, 15(3): 38-.
[9]	Dongjie Shang, Jianfei Peng, Song Guo, Zhijun Wu, Min Hu. Secondary aerosol formation in winter haze over the Beijing-Tianjin-Hebei Region, China[J]. Front. Environ. Sci. Eng., 2021, 15(2): 34-.
[10]	Jianmei Zou, Jianzhi Huang, Huichun Zhang, Dongbei Yue. Evolution of humic substances in polymerization of polyphenol and amino acid based on non-destructive characterization[J]. Front. Environ. Sci. Eng., 2021, 15(1): 5-.
[11]	Xuewen Yi, Zhanqi Gao, Lanhua Liu, Qian Zhu, Guanjiu Hu, Xiaohong Zhou. Acute toxicity assessment of drinking water source with luminescent bacteria: Impact of environmental conditions and a case study in Luoma Lake, East China[J]. Front. Environ. Sci. Eng., 2020, 14(6): 109-.
[12]	Lingchen Kong, Xitong Liu. Emerging electrochemical processes for materials recovery from wastewater: Mechanisms and prospects[J]. Front. Environ. Sci. Eng., 2020, 14(5): 90-.
[13]	Hanzhong Jia, Yafang Shi, Xiaofeng Nie, Song Zhao, Tiecheng Wang, Virender K. Sharma. Persistent free radicals in humin under redox conditions and their impact in transforming polycyclic aromatic hydrocarbons[J]. Front. Environ. Sci. Eng., 2020, 14(4): 73-.
[14]	Jing Li, Haiqin Yu, Xue Zhang, Rixin Zhu, Liangguo Yan. Crosslinking acrylamide with EDTA-intercalated layered double hydroxide for enhanced recovery of Cr(VI) and Congo red: Adsorptive and mechanistic study[J]. Front. Environ. Sci. Eng., 2020, 14(3): 52-.
[15]	Youfang Chen, Yimin Zhou, Xinyi Zhao. PM_2.5 over North China based on MODIS AOD and effect of meteorological elements during 2003‒2015[J]. Front. Environ. Sci. Eng., 2020, 14(2): 23-.

Viewed

Full text

Abstract

Cited

Shared

Discussed