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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2018, Vol. 12 Issue (5) : 10    https://doi.org/10.1007/s11783-018-1056-5
RESEARCH ARTICLE
Effect of 2-butenal manufacture wastewater to methanogenic activity and microbial community
Guangqing Song1,2,3, Hongbo Xi2,3(), Xiumei Sun1,2,3, Yudong Song2,3, Yuexi Zhou2,3()
1. College of Water sciences, Beijing Normal University, Beijing 100875, China
2. Research Center of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China
3. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environment Sciences, Beijing 100012, China
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Abstract

The inhibition ratio sharply increased with the increasing COD.

The absorbance of UV-vis at 420 nm showed a linear correlation with the SMA.

The molecular structure of EPS has changed when COD was 9585 mg/L.

Illumina Miseq sequencing was employed to reveal the microbial composition.

The synthesis of 2-butenal, which is a vital raw material for the production of sorbic acid as a food preservative, generates some toxic by-products, so it is urgent to seek better detoxification strategies for the treatment of 2-butenal manufacture wastewater. In this study, batch experiments were carried out to investigate the inhibition effect of wastewater on the methanogenic activity. To understand the wastewater toxicity to anaerobic granular sludge, variations of the specific methanogenic activity (SMA) and extracellular polymeric substance (EPS) constituents at various wastewater CODs were investigated. Ultraviolet-visible (UV-vis) spectra and Fourier transform infrared (FT-IR) spectra were employed to analyze the structure of the EPS. The results showed that the inhibitory ratio of 2-butenal manufacture wastewater was less than 8.4% on the anaerobic granular sludge when the CODs were less than 959 mg/L. However, the inhibitory ratio increased from 36.4% to 93.6% when CODs increased from 2396 mg/L to 9585 mg/L, with the SMA decreasing from 39.1 mL CH4/(gVSS·d) to 3.2 mL CH4/(gVSS·d). The diversity of the microbial community under various CODs was researched by Illumina 16S rRNA Miseq sequencing and the results demonstrated that ProteiniphilumPetrimonas and Syntrophobacter were the dominant bacteria genera in all sample. Regarding archaea, Methanobacterium was the most dominated archaea genera, followed by the Methanosaeta group in all samples. Moreover, the bacterial communities had changed obviously with increasing CODs, which indicated high CODs played a negative impact on the richness and diversity of bacterial community in the sludge samples.

Keywords 2-butenal manufacture wastewater      Methanogenic activity      Specific methanogenic activity (SMA)      Extracellular polymeric substance (EPS)      Microbial community     
Corresponding Author(s): Hongbo Xi,Yuexi Zhou   
Issue Date: 23 August 2018
 Cite this article:   
Guangqing Song,Hongbo Xi,Xiumei Sun, et al. Effect of 2-butenal manufacture wastewater to methanogenic activity and microbial community[J]. Front. Environ. Sci. Eng., 2018, 12(5): 10.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-018-1056-5
https://academic.hep.com.cn/fese/EN/Y2018/V12/I5/10
Parameter pH COD
(mg/L)
BOD5
(mg/L)b
TOC
(mg/L)
Alkalinity
(mg/L as CaCO3)
TDS
(mg/L)
SS
(mg/L)
Value 4.9±0.5 108421±568 22768±182 39283±80 248.5±13.9 14952±280 137±17
Tab.1  Characteristics of 2-butenal manufacture wastewatera
Fig.1  Schematic diagram of experimental apparatus
Fig.2  Effects of cumulative methane production (a) and the methanogenic activity (b) at various CODs
Fig.3  Variations of pH and VFA concentration profiles of batch-feed reactor conditions
Fig.4  Variations of polysaccharide and protein with the increasing wastewater CODs
Fig.5  Variations of UV-vis spectra of EPS (a) and relation between UV-vis absorbance of F420 and SMA of anaerobic sludge under different CODs of wastewater (b)
Fig.6  Changes in the functional groups of the EPS with the increasing wastewater CODs
Fig.7  Heatmap figure of the top 50 most abundance of samples with different CODs at genera level
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