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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2018, Vol. 12 Issue (5) : 15    https://doi.org/10.1007/s11783-018-1080-5
RESEARCH ARTICLE
Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the treatment of micro-polluted water
Bingjie Xu1,2, Gang Xue2(), Xing Yang2
1. College of Chemistry and Environmental Engineering, Jiujiang University, Jiujiang 332005, China
2. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China
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Abstract

• An ibuprofen-degrading strain, Serratia marcescens BL1, was isolated and identified.

• The effects of various factors on ibuprofen degradation by BL1 were evaluated.

• Strain BL1 was applied to a laboratory-scale biological aerated filter system.

• Strain BL1 was stable in both static tests and in the biological aerated filter system.

Ibuprofen (IBU) is widely used in the world as anti-inflammatory drug, which posed health risk to the environment. A bacterium capable of degrading IBU was isolated from activated sludge in a sewage treatment plant. According to its morphological, physiologic, and biochemical characteristics, as well as 16S rRNA sequence analysis, the strain was identified as Serratia marcescens BL1 (BL1). Degradation of IBU required the presence of primary substrate. After a five-day cultivation with yeast powder at 30℃ and pH 7, the highest degradation (93.47%±2.37%) was achieved. The process of BL1 degrading IBU followed first-order reaction kinetics. The BL1 strain was applied to a small biological aerated filter (BAF) device to form a biofilm with activated sludge. IBU removal by the BAF was consistent with the results of static tests. The removal of IBU was 32.01% to 44.04% higher than for a BAF without BL1. The indigenous bacterial community was able to effectively remove CODMn (permanganate index) and ammonia nitrogen in the presence of BL1.

Keywords Ibuprofen      Biological aerated filter      Degrading bacterium      Serratia marcescens     
Corresponding Author(s): Gang Xue   
Issue Date: 10 September 2018
 Cite this article:   
Bingjie Xu,Gang Xue,Xing Yang. Isolation and application of an ibuprofen-degrading bacterium to a biological aerated filter for the treatment of micro-polluted water[J]. Front. Environ. Sci. Eng., 2018, 12(5): 15.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-018-1080-5
https://academic.hep.com.cn/fese/EN/Y2018/V12/I5/15
Fig.1  Scanning electron micrograph of strain BL1
Fig.2  Phylogenetic tree of strain BL1 based on 16S rDNA sequence (Numbers at the nodes indicate bootstrap percentages for 1000 replicates. The scale bars represent 100 substitutions/site)
Fig.3  Effect of methanol on the degradation process of IBU by strain BL1
Fig.4  Effect of nutrients on the degradation process of IBU by strain BL1
Primary substrate Kinetic equation K (d-1) t1/2 (d) P-value R2
Glucose lnCa) = -0.39tb) + 3.50 0.39 1.78 <0.0002 0.97
Peptone lnC = -0.42t + 3.46 0.42 1.65 <0.0002 0.95
Yeast lnC = -0.58t + 3.57 0.58 1.20 <0.0002 0.99
Tab.1  Kinetic equations and parameters for the IBU removal under different primary substrate conditions
Fig.5  Effect of initial pH and temperature on the degradation efficiency of IBU by strain BL1: (a) pH; (b) temperature
Fig.6  Removal efficiencies of conventional pollution indicators and IBU by BAF: (a) CODMn and ammonia nitrogen; (b) IBU
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