Removal of trimethoprim and sulfamethoxazole in artificial composite soil treatment systems and diversity of microbial communities

doi:10.1007/s11783-019-1112-9

Front. Environ. Sci. Eng.

2019, Vol. 13

Issue (2) : 28 https://doi.org/10.1007/s11783-019-1112-9

RESEARCH ARTICLE

Removal of trimethoprim and sulfamethoxazole in artificial composite soil treatment systems and diversity of microbial communities

Qinqin Liu^1,², Miao Li²(

), Rui Liu², Quan Zhang², Di Wu³, Danni Zhu⁴, Xuhui Shen¹, Chuanping Feng⁵, Fawang Zhang⁴, Xiang Liu²(

)

¹. The Institute of Crustal Dynamics, China Earthquake Administration, Beijing 100085, China
². School of Environment, Tsinghua University, Beijing 100084, China
³. Satellite Environmental Center, Ministry of Environmental Protection, Beijing 100092, China
⁴. Institute of Karst Geology, CAGS/Key Laboratory of Karst Dynamics, MLR&GZAR, Guilin 541004, China
⁵. China University of Geosciences (Beijing), Beijing 100083, China

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Abstract

• Novel ACST allowed biodegradation to effectively remove adsorbed SMX and TMP.

• Ammonia and nitrite were efficiently removed in ACSTs and water quality was improved.

Four artificial composite soil treatment systems (ACSTs) fed with reclaimed water containing trimethoprim (TMP) and sulfamethoxazole (SMX) were constructed to investigate SMX and TMP biodegradation efficiency, ammonia and nitrite removal conditions and the microbial community within ACST layers. Results showed SMX and TMP removal rates could reach 80% and 95%, respectively, and removal rates of ammonia and nitrite could reach 80% and 90%, respectively, in ACSTs. The MiSeq sequencing results showed that microbial community structures of the ACSTs were similar. The dominant microbial community in the adsorption and biodegradation layers of the ACSTs contained Proteobacteria, Chloroflexi, Acidobacteria, Firmicutes, Actinobacteria and Nitrospirae. Firmicutes and Proteobacteria were considerably dominant in the ACST biodegradation layers. The entire experimental results indicated that Nitrosomonadaceae_uncultured, Nitrospira and Bacillus were associated with nitrification processes, while Bacillus and Lactococcus were associated with SMX and TMP removal processes. The findings suggest that ACSTs are appropriate for engineering applications.

Keywords Trimethoprim Sulfamethoxazole Reclaimed water Biodegradation Aerobic nitrification Microbial community

Corresponding Author(s): Miao Li,Xiang Liu

Issue Date: 26 March 2019

Cite this article:

Qinqin Liu,Miao Li,Rui Liu, et al. Removal of trimethoprim and sulfamethoxazole in artificial composite soil treatment systems and diversity of microbial communities[J]. Front. Environ. Sci. Eng., 2019, 13(2): 28.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-019-1112-9
https://academic.hep.com.cn/fese/EN/Y2019/V13/I2/28

Fig.1 Schematic diagram of the artificial composite soil column. The values are given in millimeter. The column inner diameter is 90 mm. The particle sizes of the coarse medium sands, silty clay, clay ceramsites, and volcanic material is about 0.5–1,<0.2, 2–3, and 1–2 mm, respectively. In the supporting layer, the particle sizes of graded cobblestones are about 2–5 cm.

Tab.1 The experimental scheme of reclaimed water recharge process

Fig.2 The concentration variations and removal rates of SMX and TMP. (b) and (d) are removal rates of SMX and TMP for columns 1, 2, 3 and 4. (a) and (c) are the influent and effluent concentrations of SMX and TMP for columns 1, 2, 3 and 4. Y1 represents the influent concentrations of SMX and TMP for columns 1, 2 and 3, while Y2 represents the influent concentrations of SMX and TMP for column 4. Columns 1, 2, 3 and 4 represent the effluent concentrations of SMX and TMP for columns 1, 2, 3 and 4.

Fig.3 The variation of pH and DO influent and effluent concentrations. Y represents the influent concentrations of pH and DO for columns 1, 2 and 3. Columns 1, 2 and 3 represent the effluent concentrations of pH and DO for columns 1, 2 and 3, respectively.

Fig.4 The variation of influent and effluent concentrations of NH₄⁺-N, NO₂⁻-N and NO₃⁻-N. Y represents the influent concentrations of NH₄⁺-N, NO₂⁻-N and NO₃⁻-N for columns 1, 2 and 3. Columns 1, 2 and 3 represent the effluent concentrations of NH₄⁺-N, NO₂⁻-N and NO₃⁻-N for columns 1, 2, and 3, respectively.

Tab.2 Alpha-diversity indices of three samples in ACST

Fig.5 Abundances of the major phyla in ACST samples. A1, B1 and C1 represent the composite soil samples of the middle of adsorption and biodegradation layers in ACST1, 2 and 3. A2 and C2 represent the composite soil samples of the middle of adsorption layers of ACST1 and ACST2.

Fig.6 Heatmap of the major genera in ACST samples. A1, B1 and C1 represent the composite soil samples of the middle of adsorption and biodegradation layers in ACST1, 2 and 3. A2 and C2 represent the composite soil samples of the middle of adsorption layers of ACST1 and ACST2.

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