Please wait a minute...
Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2020, Vol. 14 Issue (3) : 53    https://doi.org/10.1007/s11783-020-1230-4
RESEARCH ARTICLE
A novel strategy for gas mitigation during swine manure odour treatment using seaweed and a microbial consortium
Madhavaraj Lavanya1, Ho-Dong Lim1, Kong-Min Kim1, Dae-Hyuk Kim1,2, Balasubramani Ravindran3, Gui Hwan Han1()
1. Center for Industrialization of Agricultural and Livestock Microorganisms (CIALM), Jeongeup-si 56212, Republic of Korea
2. Department of Molecular Biology, Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju 561-756, Republic of Korea
3. Department of Environmental Energy & Engineering, Kyonggi University, Suwon-si 16227, Republic of Korea
 Download: PDF(1809 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract

• Comprehensive mitigation of gas emissions from swine manure was investigated.

• Additives addition for mitigation of gas from the manure has been developed.

Sargassum horneri, seaweed masking strategy controlled gas by 90%-100%.

• Immediate reduction in emitted gas and improving air quality has been determined.

• Microbial consortium with seaweed completely controlled gas emissions by 100%.

Gas emissions from swine farms have an impact on air quality in the Republic of Korea. Swine manure stored in deep pits for a long time is a major source of harmful gas emissions. Therefore, we evaluated the mitigation of emissions of ammonia (NH3), hydrogen sulfide (H2S) and amine gases from swine manure with biological products such as seaweed (Sargassum horneri) and a microbial consortium (Bacillus subtilis (1.2 × 109 CFU/mL), Thiobacillus sp. (1.0 × 1010 CFU/mL) and Saccharomyces cerevisiae (2.0 × 109 CFU/mL)) used as additives due to their promising benefits for nutrient cycling. Overall, seaweed powder masking over two days provided notable control of over 98%-100% of the gas emissions. Furthermore, significant control of gas emissions was especially pronounced when seaweed powder masking along with a microbial consortium was applied, resulting in a gas reduction rate of 100% for NH3, amines and H2S over 10 days of treatment. The results also suggested that seaweed powder masking and a microbial consortium used in combination to reduce the gas emissions from swine manure reduced odour compared with that observed when the two additives were used alone. Without the consortium, seaweed decreased total volatile fatty acid (VFA) production. The proposed novel method of masking with a microbial consortium is promising for mitigating hazardous gases, simple, and environmentally beneficial. More research is warranted to determine the mechanisms underlying the seaweed and substrate interactions.

Keywords Seaweed      Consortium      Mitigation      Ammonia      H2S      Volatile fatty acids (VFAs)     
Corresponding Author(s): Gui Hwan Han   
Issue Date: 26 March 2020
 Cite this article:   
Madhavaraj Lavanya,Ho-Dong Lim,Kong-Min Kim, et al. A novel strategy for gas mitigation during swine manure odour treatment using seaweed and a microbial consortium[J]. Front. Environ. Sci. Eng., 2020, 14(3): 53.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1230-4
https://academic.hep.com.cn/fese/EN/Y2020/V14/I3/53
Treatment Swine slurry (mL) Water (mL) Additives Percentage tested
(%)
Treatment 1
Control
T1
T2
T3
200
200
200
200
300
300
300
300

Bacillus subtilis
Saccharomyces cerevisiae
Thiobacillus sp.

(0.5–2)
(0.5–2)
(0.5–2)
Treatment 2
Control
Consortium
200
200
300
300

(B. subtilis+ S. cerevisiae+
Thiobacillus sp.)

(1.5+ 1.5+ 2)
Treatment 3
Control
Seaweed
200
200
300
300

Sargassum horneri

(0.5, 1, 1.5, 2, 5, 10)
Tab.1  Design for determining the optimum raw materials for odour reduction
Fig.1  Evaluation of the optimal bacterial composition for reducing odorous gases (1. ammonia, 2. amines, and 3. H2S) from swine manure. (a) B. subtilis; (b) S. cerevisiae; (c) Thiobacillus sp. Data represent the averages of three replicates, and error bars show standard deviations.
Fig.2  Effects of the consortium (B. subtilis (1.5%), S. cerevisiae (1.5%) and Thiobacillus sp.(2%), v/v) treatment on (a) ammonia, (b) amines, and (c) H2S released during the process of reducing odours emitted by swine manure. Data represent the averages of three replicates, and error bars show standard deviations.
Fig.3  Effects of seaweed (SW) (0.5%, 1%, 1.5%, 2%, 5%, and 10%, w/v) treatment on (a) ammonia, (b) amines, and (c) H2S released during the process of reducing odours emitted by swine manure. Data represent the averages of three replicates, and error bars show standard deviations.
Fig.4  Comparison of the MC (addition of the microbial consortium (B. subtilis (1.5%), S. cerevisiae (1.5%) and Thiobacillus sp.(2%), w/v)), SW (seaweed (2%) masking), and SW+ MC (first consortium (B. subtilis (1.5%), S. cerevisiae (1.5%) and Thiobacillus sp.(2%), w/v) followed by seaweed (2%) masking) treatments with the control (no treatment of swine manure) to assess the reduction of (a) ammonia, (b) amine, (c) and H2S gases emitted during the process of reducing odours emitted by swine manure. Data represent the averages of three replicates, and error bars show standard deviations.
Fig.5  Total bacterial population size after 10 days of treatment. Comparison of samples from treatments MC (addition of the consortium (B. subtilis (1.5%), S. cerevisiae (1.5%) and Thiobacillus sp. (2%), w/v), SW (seaweed (2%) masking), and SW+ MC (first consortium followed by seaweed (2%) masking) with samples from the control (no treatment) during the process of reducing odours emitted by swine manure. Data represent the averages of three replicates, and error bars show standard deviations.
Fig.6  Evaluation of butyric acid (a), iso-butyric acid (b), valeric acid (c), iso-valeric acid (d), acetic acid (e) and propionic acid (f) over 10 days of odour reduction treatment of swine manure. Control: swine manure alone; test 1: addition of the consortium (B. subtilis (1.5%) + S. cerevisiae (1.5%) + Thiobacillus sp.(2%), w/v); test 2: seaweed (2%) masking; test 3: first consortium followed by seaweed (2%) masking. Data represent the averages of three replicates, and error bars show standard deviations.
NH3 - ammonia
H2S- hydrogen sulfide
HCl- hydrochloric acid
mL- milliliter
mg/L- milligrams per liter
CFU/mL- colony-forming units per milliliter
%, w/v- percent, weight/volume
v/v- volume/volume
mL- microliter
µm- micrometer
m- meter
mm- millimeter
  
1 American Public Health Association, Eaton A D, American Water Works Association, Water Environment Federation (2005). Standard Methods for the Examination of Water and Wastewater. Washington, DC: APHA-AWWA-WEF
2 M K Awasthi , Z Zhang , Q Wang , F Shen , R Li , D S Li , X Ren , M Wang , H Chen , J Zhao (2017). New insight with the effects of biochar amendment on bacterial diversity as indicators of biomarkers support the thermophilic phase during sewage sludge composting. Bioresource Technology, 238: 589–601
https://doi.org/10.1016/j.biortech.2017.04.100
3 R Badar , M Khan , B Batool , S Shabbir (2015). Effects of organic amendments in comparison with chemical fertilizer on cowpea growth. International Journal of Applied Research, 1(5): 66–71
4 M Benjamin , S Yik (2019). Precision livestock farming in swine welfare: A review for swine practitioners. Animals (Basel), 9(4): 133–154
https://doi.org/10.3390/ani9040133
5 M A Chowdhury , A de Neergaard , L S Jensen (2014). Potential of aeration flow rate and bio-char addition to reduce greenhouse gas and ammonia emissions during manure composting. Chemosphere, 97: 16–25
https://doi.org/10.1016/j.chemosphere.2013.10.030
6 K L Conn , M Tenuta , G Lazarovits (2005). Liquid swine manure can kill Verticillium dahliae microsclerotia in soil by volatile fatty acid, nitrous acid, and ammonia toxicity. Phytopathology, 95(1): 28–35
https://doi.org/10.1094/PHYTO-95-0028
7 C Dennehy , P G Lawlor , Y Jiang , G E Gardiner , S Xie , L D Nghiem , X Zhan (2017). Greenhouse gas emissions from different pig manure management techniques: A critical analysis. Frontiers of Environmental Science & Engineering, 11(3): 11
https://doi.org/10.1007/s11783-017-0942-6
8 X Ding , X Han , Y Liang , Y Qiao , L Li , N Li (2012). Changes in soil organic carbon pools after 10 years of continuous manuring combined with chemical fertilizer in a Mollisol in China. Soil & Tillage Research, 122: 36–41
https://doi.org/10.1016/j.still.2012.02.002
9 D Domozych , M Ciancia , J Fangel , M Mikkelsen , P Ulvskov , W Willats (2012). The cell walls of green algae: A journey through evolution and diversity. Frontiers of Plant Science, 3: 82
https://doi.org/10.3389/fpls.2012.00082
10 L Dufossé, P Galaup , A Yaron , S M Arad , P Blanc , K N Chidambara Murthy , G A Ravishankar (2005). Microorganisms and microalgae as sources of pigments for food use: A scientific oddity or an industrial reality? Trends in Food Science & Technology, 16(9): 389–406
https://doi.org/10.1016/j.tifs.2005.02.006
11 A Feilberg , D Liu , A P Adamsen , M J Hansen , K E Jonassen (2010). Odorant emissions from intensive pig production measured by online proton-transfer-reaction mass spectrometry. Environmental Science & Technology, 44(15): 5894–5900
https://doi.org/10.1021/es100483s
12 R Jumaidin , S Sapuan , M Jawaid , M Ishak , J Sahari (2016). Effect of seaweed on physical properties of thermoplastic sugar palm starch/agar composites. Journal of Mechanical Engineering Science, 10(3): 2214–2225
13 J A Kim , J Bayo , J Cha , Y J Choi , M Y Jung , D H Kim , Y Kim (2019). Investigating the probiotic characteristics of four microbial strains with potential application in feed industry. PLoS One, 14(6): e0218922
https://doi.org/10.1371/journal.pone.0218922
14 K Kuroda , A Tanaka , K Furuhashi , K Nakasaki (2017). Application of Bacillus sp. TAT105 to reduce ammonia emissions during pilot-scale composting of swine manure. Bioscience, Biotechnology, and Biochemistry, 81(12): 2400–2406
https://doi.org/10.1080/09168451.2017.1389607
15 P D Le , A J Aarnink , N W Ogink , P M Becker , M W Verstegen (2005). Odour from animal production facilities: Its relationship to diet. Nutrition Research Reviews, 18(1): 3–30
https://doi.org/10.1079/NRR200592
16 L Machado , M Magnusson , N A Paul , R de Nys , N Tomkins (2014). Effects of marine and freshwater macroalgae on in vitro total gas and methane production. PLoS One, 9(1): e85289
https://doi.org/10.1371/journal.pone.0085289
17 M R G Maia , A J M Fonseca , H M Oliveira , C Mendonça , A R J Cabrita (2016). The potential role of seaweeds in the natural manipulation of rumen fermentation and methane production. Scientific Reports, 6(1): 32321
https://doi.org/10.1038/srep32321
18 S Mandal , R Thangarajan , N S Bolan , B Sarkar , N Khan , Y S Ok , R Naidu (2016). Biochar-induced concomitant decrease in ammonia volatilization and increase in nitrogen use efficiency by wheat. Chemosphere, 142: 120–127
https://doi.org/10.1016/j.chemosphere.2015.04.086
19 C E Manyi-Loh , S N Mamphweli , E L Meyer , G Makaka , M Simon , A I Okoh (2016). An overview of the control of bacterial pathogens in cattle manure. International Journal of Environmental Research and Public Health, 13(9): 843
https://doi.org/10.3390/ijerph13090843
20 H Matsumura , M Sasaki , S I Kato , K Nakasaki (2010). Unusual effects of triacylglycerol on the reduction of ammonia gas emission during thermophilic composting. Bioresource Technology, 101(7): 2300–2305
https://doi.org/10.1016/j.biortech.2009.11.006
21 D Maurer , J Koziel , K Kalus , D Andersen , S Opaliński (2017). Pilot-scale testing of non-activated biochar for swine manure treatment and mitigation of ammonia, hydrogen sulfide, odorous Volatile Organic Compounds (VOCs), and greenhouse gas emissions. Sustainability, 9(6): 929
https://doi.org/10.3390/su9060929
22 G W Miller , G S Patterson (2002). Treatment of Animal Waste. United States Patent No.: US 6,410,305. Maple Grove: BioSun Systems Corporation
23 C L Okolie, S R C K Rajendran, C C Udenigwe, A N A Aryee, B Mason (2017). Prospects of brown seaweed polysaccharides (BSP) as prebiotics and potential immunomodulators. Journal of Food Biochemistry, 41(5): e12392
https://doi.org/10.1111/jfbc.12392
24 P J Olson , Y K Ban (2018). Livestock and products semi-annual. GAIN Report KS1810. Seoul: Global Agricultural Information Network
25 C G Orpin , Y Greenwood , F J Hall , I W Paterson (1985). The rumen microbiology of seaweed digestion in Orkney sheep. Journal of Applied Bacteriology, 58(6): 585–596
https://doi.org/10.1111/j.1365-2672.1985.tb01715.x
26 A Pacholczak , K Nowakowska , S Pietkiewicz (2016). The effects of synthetic auxin and a seaweed-based biostimulator on physiological aspects of rhizogenesis in ninebark stem cuttings. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(1): 85–91
https://doi.org/10.15835/nbha44110061
27 O Pulz , W Gross (2004). Valuable products from biotechnology of microalgae. Applied Microbiology and Biotechnology, 65(6): 635–648
https://doi.org/10.1007/s00253-004-1647-x
28 Z Qi , H Liu , B Li , Y Mao , Z Jiang , J Zhang , J Fang (2010). Suitability of two seaweeds, Gracilaria lemaneiformis and Sargassum pallidum, as feed for the abalone Haliotis discus hannai Ino. Aquaculture (Amsterdam, Netherlands), 300(1–4): 189–193
https://doi.org/10.1016/j.aquaculture.2010.01.019
29 B Ravindran , D D Nguyen , D K Chaudhary , S W Chang , J Kim , S R Lee , J Shin , B H Jeon , S Chung , J Lee (2019). Influence of biochar on physico-chemical and microbial community during swine manure composting process. Journal of Environmental Management, 232: 592–599
https://doi.org/10.1016/j.jenvman.2018.11.119
30 K K A Sanjeewa , I P S Fernando , S Y Kim , H S Kim , G Ahn , Y Jee , Y J Jeon (2018). In vitro and in vivo anti-inflammatory activities of high molecular weight sulfated polysaccharide; containing fucose separated from Sargassum horneri: Short communication. International Journal of Biological Macromolecules, 107 (Part A): 803–807
https://doi.org/10.1016/j.ijbiomac.2017.09.050
31 C Sundberg, W A Al-Soud, M Larsson, E Alm, S S Yekta, B H Svensson, S J Sørensen, A Karlsson (2013). 454-pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters. FEMS Microbiology Ecology, 85(3): 612–626
https://doi.org/10.1111/1574-6941.12148
32 J A Zahn , A A DiSpirito , Y S Do , B E Brooks , E E Cooper , J L Hatfield (2001). Correlation of human olfactory responses to airborne concentrations of malodorous volatile organic compounds emitted from swine effluent. Journal of Environmental Quality, 30(2): 624–634
https://doi.org/10.2134/jeq2001.302624x
33 W Zhang , A Lau (2007). Reducing ammonia emission from poultry manure composting via struvite formation. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 82(6): 598–602
https://doi.org/10.1002/jctb.1701
34 J Zhu (2000). A review of microbiology in swine manure odor control. Agriculture, Ecosystems & Environment, 78(2): 93–106
https://doi.org/10.1016/S0167-8809(99)00116-4
[1] FSE-20001-OF-LM_suppl_1 Download
[1] Jian Lu, Cui Zhang, Jun Wu. Removal of steroid hormones from mariculture system using seaweed Caulerpa lentillifera[J]. Front. Environ. Sci. Eng., 2022, 16(2): 15-.
[2] Yukun Zhang, Shuying Wang, Shengbo Gu, Liang Zhang, Yijun Dong, Lei Jiang, Wei Fan, Yongzhen Peng. The combined effects of biomass and temperature on maximum specific ammonia oxidation rate in domestic wastewater treatment[J]. Front. Environ. Sci. Eng., 2021, 15(6): 123-.
[3] Noshan Bhattarai, Shuxiao Wang, Yuepeng Pan, Qingcheng Xu, Yanlin Zhang, Yunhua Chang, Yunting Fang. δ15N-stable isotope analysis of NHx: An overview on analytical measurements, source sampling and its source apportionment[J]. Front. Environ. Sci. Eng., 2021, 15(6): 126-.
[4] Yingwu Wang, Ping Ning, Ruheng Zhao, Kai Li, Chi Wang, Xin Sun, Xin Song, Qiang Lin. A Cu-modified active carbon fiber significantly promoted H2S and PH3 simultaneous removal at a low reaction temperature[J]. Front. Environ. Sci. Eng., 2021, 15(6): 132-.
[5] Shaoyi Xu, Xiaolong Wu, Huijie Lu. Overlooked nitrogen-cycling microorganisms in biological wastewater treatment[J]. Front. Environ. Sci. Eng., 2021, 15(6): 133-.
[6] Shiguan Yang, Xinrui Fan, Ji Liu, Wei Zhao, Bin Hu, Qiang Lu. Mechanism insight into the formation of H2S from thiophene pyrolysis: A theoretical study[J]. Front. Environ. Sci. Eng., 2021, 15(6): 120-.
[7] Zeshen Tian, Bo Wang, Yuyang Li, Bo Shen, Fengjuan Li, Xianghua Wen. Enhancement on the ammonia oxidation capacity of ammonia-oxidizing archaeon originated from wastewater: Utilizing low-density static magnetic field[J]. Front. Environ. Sci. Eng., 2021, 15(5): 81-.
[8] Kuo Fang, Fei Peng, Hui Gong, Huanzhen Zhang, Kaijun Wang. Ammonia removal from low-strength municipal wastewater by powdered resin combined with simultaneous recovery as struvite[J]. Front. Environ. Sci. Eng., 2021, 15(1): 8-.
[9] Shuo Wei, Lei Du, Shuo Chen, Hongtao Yu, Xie Quan. Electro-assisted CNTs/ceramic flat sheet ultrafiltration membrane for enhanced antifouling and separation performance[J]. Front. Environ. Sci. Eng., 2021, 15(1): 11-.
[10] Alisa Salimova, Jian’e Zuo, Fenglin Liu, Yajiao Wang, Sike Wang, Konstantin Verichev. Ammonia and phosphorus removal from agricultural runoff using cash crop waste-derived biochars[J]. Front. Environ. Sci. Eng., 2020, 14(3): 48-.
[11] Chunhong Chen, Hong Liang, Dawen Gao. Community diversity and distribution of ammonia-oxidizing archaea in marsh wetlands in the black soil zone in North-east China[J]. Front. Environ. Sci. Eng., 2019, 13(4): 58-.
[12] Yanqing Duan, Aijuan Zhou, Kaili Wen, Zhihong Liu, Wenzong Liu, Aijie Wang, Xiuping Yue. Upgrading VFAs bioproduction from waste activated sludge via co-fermentation with soy sauce residue[J]. Front. Environ. Sci. Eng., 2019, 13(1): 3-.
[13] Yao Zhang, Yayi Wang, Yuan Yan, Haicheng Han, Min Wu. Characterization of CANON reactor performance and microbial community shifts with elevated COD/N ratios under a continuous aeration mode[J]. Front. Environ. Sci. Eng., 2019, 13(1): 7-.
[14] Kit Wayne Chew, Pau Loke Show, Yee Jiun Yap, Joon Ching Juan, Siew Moi Phang, Tau Chuan Ling, Jo-Shu Chang. Sonication and grinding pre-treatments on Gelidium amansii seaweed for the extraction and characterization of Agarose[J]. Front. Environ. Sci. Eng., 2018, 12(4): 2-.
[15] Mengqian Lu, Bin-Le Lin, Kazuya Inoue, Zhongfang Lei, Zhenya Zhang, Kiyotaka Tsunemi. PM2.5-related health impacts of utilizing ammonia-hydrogen energy in Kanto Region, Japan[J]. Front. Environ. Sci. Eng., 2018, 12(2): 13-.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed