Photosynthesis and related metabolic mechanism of promoted rice (Oryza sativa L.) growth by TiO2 nanoparticles

doi:10.1007/s11783-020-1282-5

Front. Environ. Sci. Eng.

2020, Vol. 14

Issue (6) : 103 https://doi.org/10.1007/s11783-020-1282-5

RESEARCH ARTICLE

Photosynthesis and related metabolic mechanism of promoted rice (Oryza sativa L.) growth by TiO₂ nanoparticles

Yingdan Zhang^1,², Na Liu^1,², Wei Wang^1,², Jianteng Sun^1,³, Lizhong Zhu^1,²(

)

¹. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China
². Zhejiang Provincial Key Laboratory of Organic Pollution Process and Control, Hangzhou 310058, China
³. School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China

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Abstract

• The rice growth was promoted by nano-TiO₂ of 0.1–100 mg/L.

• Nano-TiO₂ enhanced the energy storage in photosynthesis.

• Nano-TiO₂ reduced energy consumption in carbohydrate metabolism and TCA cycle.

Titanium dioxide nanoparticle (nano-TiO₂), as an excellent UV absorbent and photo-catalyst, has been widely applied in modern industry, thus inevitably discharged into environment. We proposed that nano-TiO₂ in soil can promote crop yield through photosynthetic and metabolic disturbance, therefore, we investigated the effects of nano-TiO₂ exposure on related physiologic-biochemical properties of rice (Oryza sativa L.). Results showed that rice biomass was increased >30% at every applied dosage (0.1–100 mg/L) of nano-TiO₂. The actual photosynthetic rate (Y(II)) significantly increased by 10.0% and 17.2% in the treatments of 10 and 100 mg/L respectively, indicating an increased energy production from photosynthesis. Besides, non-photochemical quenching (Y(NPQ)) significantly decreased by 19.8%–26.0% of the control in all treatments respectively, representing a decline in heat dissipation. Detailed metabolism fingerprinting further revealed that a fortified transformation of monosaccharides (D-fructose, D-galactose, and D-talose) to disaccharides (D-cellobiose, and D-lactose) was accompanied with a weakened citric acid cycle, confirming the decrease of energy consumption in metabolism. All these results elucidated that nano-TiO₂ promoted rice growth through the upregulation of energy storage in photosynthesis and the downregulation of energy consumption in metabolism. This study provides a mechanistic understanding of the stress-response hormesis of rice after exposure to nano-TiO₂, and provides worthy information on the potential application and risk of nanomaterials in agricultural production.

Keywords Nano-TiO₂ Rice Photosynthesis Metabolomics Energy storage

Corresponding Author(s): Lizhong Zhu

Issue Date: 11 June 2020

Cite this article:

Yingdan Zhang,Na Liu,Wei Wang, et al. Photosynthesis and related metabolic mechanism of promoted rice (Oryza sativa L.) growth by TiO₂ nanoparticles[J]. Front. Environ. Sci. Eng., 2020, 14(6): 103.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1282-5
https://academic.hep.com.cn/fese/EN/Y2020/V14/I6/103

Fig.1 Effects of different concentrations of nano-titanium dioxide on the biomass of rice plants after exposure for 21 days (a) and the titanium content of rice leaves after exposure at 21 days (b). The values are presented as means±SE (n = 3). Points denoted by different lower case letters on each curve differ significantly at p<0.05. The same lower case letter is not significantly different for a particular dose.

Fig.2 Photosynthesis parameters of rice leaves after exposure to nano-TiO₂ for 21-day. Values are presented as means±SE (n = 3). Points denoted by different lower case letters on each bar differ significantly at p<0.05. The same lower case letter is not significantly different for a particular dose.

Fig.3 Effect of nano-TiO₂ on rice analyzed by metabolic analysis system 4.0. Principal component analysis (PCA) (a) and enrichment analysis of metabolites in rice leaves treated with different dosage of nano-TiO₂ (b). Every pillar represents a metabolic pathway, where red color indicates higher impact and yellow color indicates lower impact. The pathways with p-value?<?0.05 and fold enrichment>2 were determined to have significant changes.

Fig.4 Significant change of metabolites in rice leaves exposed to nano-TiO₂.

Fig.5 Relationship map of metabolites identified by GC-MS in leaves.

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