Acute toxicity assessment of drinking water source with luminescent bacteria: Impact of environmental conditions and a case study in Luoma Lake, East China

doi:10.1007/s11783-020-1288-z

Front. Environ. Sci. Eng.

2020, Vol. 14

Issue (6) : 109 https://doi.org/10.1007/s11783-020-1288-z

RESEARCH ARTICLE

Acute toxicity assessment of drinking water source with luminescent bacteria: Impact of environmental conditions and a case study in Luoma Lake, East China

Xuewen Yi¹, Zhanqi Gao², Lanhua Liu³, Qian Zhu³, Guanjiu Hu²(

), Xiaohong Zhou³(

)

¹. College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China
². State Environmental Protection Key Laboratory of Monitoring and Analysis for Organic Pollutants in Surface Water, Jiangsu Environmental Monitoring Center, Nanjing 210036, China
³. State Key Joint Laboratory of ESPC, School of Environment, Tsinghua University, Beijing 100084, China

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Abstract

• Acute toxicity assessment was conducted in Luoma lake watershed, East China.

• Impacts of environmental factors on the toxicity testing was fully evaluated.

• Dissolve oxygen had a weak positive correlation with luminescence inhibition rate.

Protecting the quality of lake watersheds by preventing and reducing their contamination is an effective approach to ensure the sustainability of the drinking water supply. In this study, acute toxicity assessment was conducted on the basis of acute bioluminescence inhibition assay using the marine bacterium Vibrio fischeri as the test organism and Luoma Lake drinking water source in East China as the research target. The suitable ranges of environmental factors, including pH value, organic matter, turbidity, hardness, and dissolved oxygen of water samples were evaluated for the toxicity testing of bioluminescent bacteria. The physicochemical characteristics of water samples at the selected 43 sites of Luoma Lake watershed were measured. Results showed that the variations in pH value (7.31–8.41), hardness (5–20 °d) and dissolved oxygen (4.44–11.03 mg/L) of Luoma Lake and its main inflow and outflow rivers had negligible impacts on the acute toxicity testing of V. fischeri. The luminescence inhibition rates ranged from -11.21% to 10.80% at the 43 sites. Pearson’s correlation analysis in the experiment revealed that temperature, pH value, hardness, and turbidity had no correlation with luminescence inhibition rate, whereas dissolved oxygen showed a weak statistically positive correlation with a Pearson correlation coefficient of 0.455 (p<0.05).

Keywords Bioluminescent bacteria Acute toxicity Pearson correlation analysis Drinking water source Vibrio fischeri

Corresponding Author(s): Guanjiu Hu,Xiaohong Zhou

Issue Date: 13 July 2020

Cite this article:

Xuewen Yi,Zhanqi Gao,Lanhua Liu, et al. Acute toxicity assessment of drinking water source with luminescent bacteria: Impact of environmental conditions and a case study in Luoma Lake, East China[J]. Front. Environ. Sci. Eng., 2020, 14(6): 109.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-020-1288-z
https://academic.hep.com.cn/fese/EN/Y2020/V14/I6/109

Fig.1 Location of Luoma Lake watershed and distribution of the sampling sites.

Tab.1 Investigated environmental factors and their ranges in this study

Fig.2 Effects of pH value (a) and humic acid concentration (b) of water samples on the toxicity testing of luminescent bacteria.

Fig.3 Effects of turbidity (a), hardness (b), and dissolved oxygen (c) of water samples with and without ZnSO₄·7H₂O on the toxicity testing.

Fig.4 Effects of nutrients nitrogen (a) and phosphorus (b) on the toxicity testing of luminescent bacteria.

Tab.2 Temperature, pH, hardness, dissolved oxygen and luminescence inhibition rate of water samples in Luoma Lake

Fig.5 Acute toxicity assessment of 43 sampling sites at Luoma Lake watershed with luminescent bacteria.

Tab.3 Pearson’s correlation coefficients (r) of typical physicochemical parameters and the inhibition rate of Luoma Lake watershed samples

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