Selective pseudosolubilization capability of <italic>Pseudomonas</italic> sp. DG17 on <italic>n</italic>-alkanes and uptake mechanisms analysis

doi:10.1007/s11783-013-0498-z

Front Envir Sci Eng

2013, Vol. 7

Issue (4) : 539-551 https://doi.org/10.1007/s11783-013-0498-z

RESEARCH ARTICLE

Selective pseudosolubilization capability of Pseudomonas sp. DG17 on n-alkanes and uptake mechanisms analysis

Fei HUA, Hongqi WANG(

)

College of Water Sciences, Beijing Normal University, Beijing 100875, China

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Abstract

Pseudosolubilized ability of Pseudomonas sp. DG17 on n-alkanes, role of biosurfactants in n-octadecane uptake and trans-membrane transport mechanism of n-octadecane were studied by analyzing amount of pseudosolubilized oil components in water phase, and the fraction of radiolabeled ¹⁴C n-octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C₁₂ to C₂₈ of n-alkanes. In n-octadecane broth, pseudosolubilized n-octadecane could be accumulated as long as pseudosolubilized rate was faster than mineralization rate of substrate, and the maximum concentration of pseudosolubilized n-octadecane achieved to 45.37 mg·L^-1. All of these results showed that Pseudomonas sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of ¹⁴C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of n-octadecane.

Keywords Pseudomonas alkane uptake pseudosolubilization trans-membrane transport

Corresponding Author(s): WANG Hongqi,Email:whongqi310@sohu.com

Issue Date: 01 August 2013

Cite this article:

Fei HUA,Hongqi WANG. Selective pseudosolubilization capability of Pseudomonas sp. DG17 on n-alkanes and uptake mechanisms analysis[J]. Front Envir Sci Eng, 2013, 7(4): 539-551.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-013-0498-z
https://academic.hep.com.cn/fese/EN/Y2013/V7/I4/539

Tab.1 Pseudosolubilizd crude oil components in the water phase by sp. DG17

Tab.2 Pseudosolubilized -alkanes components in the water phase under the effect of sp. DG17

Tab.3 -alkanes components changes in oil film by sp. DG17

Fig.1 Cell growth of sp. DG17 on -octadecane (open triangle) and surface tension changes of culture medium (open circle)

Fig.2 Content distribution of -octadecane in the medium. Total -octadecane in the broth (open triangle); pseudosolubilization of -octadecane (filled triangle); Control group(open diamond)

Fig.3 Phase-contrast micrograph of oil droplets size in the culture medium (a: crude oil droplets in the water phase after incubation for 35d-40 magnification; b: -octadecane droplets in the water phase at 48h-40 magnification). Bar represents 0.02 mm

Fig.4 Morphological changes of sp. DG17 (a) Cells grown on crude oil at 35d-30000 magnification; (b) Cells grown on 400 mg·L of octadecane at 120h-20000 magnification). Bar represents 2 μm

Fig.5 C -octadecane biodegradation by sp. DG17 in the aqueous phase (a) and cellular C (b) of sp. DG17. C in control group (open squares); Cells in the absence of NaN (open triangles); Cells in the presence of NaN (open diamonds)

Fig.6 Effect of different inhibitors on the transport of C -octadecane by sp. DG17. Cells in the absence of inhibitor (open diamonds), cells treated with 0.1 mmol·LCCCP (open squares), and cells treated with 30 mmol·L NaN (open triangles)

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