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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front. Environ. Sci. Eng.    2018, Vol. 12 Issue (2) : 4    https://doi.org/10.1007/s11783-017-0981-z
RESEARCH ARTICLE |
Effect of different carbon sources on performance of an A2N-MBR process and its microbial community structure
Dongliang Du1, Chuanyi Zhang1(), Kuixia Zhao2, Guangrong Sun1, Siqi Zou1, Limei Yuan1, Shilong He1
1. School of Environment and Spatial Informatics, China University of Mining & Technology, Xuzhou 221116, China
2. Guangdong polytechnic of Water Resources and Electric Engineering, Guangzhou 510635, China
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Abstract

The nutrient removal was higeher with short-chain fatty acids as carbon source.

Candidatus Accumulibacter was more easily enriched in A2N-MBR process.

Short-chain fatty acids were beneficial to the growth of PAOs.

Effect of different carbon sources on purification performance and change of microbial community structure in a novel A2N-MBR process were investigated. The results showed that when fed with acetate, propionate or acetate and propionate mixed (1:1) as carbon sources, the effluent COD, NH4+-N, TN and TP were lower than 30, 5, 15 and 0.5 mg·L-1, respectively. However, taken glucose as carbon source, the TP concentration of effluent reached 2.6 mg·L-1. Process analysis found that the amount of anaerobic phosphorus release would be the key factor to determine the above effectiveness. The acetate was beneficial to the growth ofCandidatus Accumulibacter associated with biological phosphorus removal, which was the main cause of high efficiency phosphorus removal in this system. In addition, it could eliminate theCandidatus Competibacter associated with glycogen-accumulating organisms and guarantee high efficiency phosphorus uptake of phosphorus accumulating organisms in the system with acetate as carbon source.

Keywords Denitrifying phosphorus removal      Alternate anaerobic/anoxic-aerobic MBR (A2N-MBR)      Carbon source      Operation characteristic      Community structure     
Corresponding Authors: Chuanyi Zhang   
Issue Date: 09 August 2017
 Cite this article:   
Dongliang Du,Chuanyi Zhang,Kuixia Zhao, et al. Effect of different carbon sources on performance of an A2N-MBR process and its microbial community structure[J]. Front. Environ. Sci. Eng., 2018, 12(2): 4.
 URL:  
http://academic.hep.com.cn/fese/EN/10.1007/s11783-017-0981-z
http://academic.hep.com.cn/fese/EN/Y2018/V12/I2/4
Fig.1  Schematic diagram of A2N-MBR system
Fig.2  Concentration changes of COD (a), Nitrogen (b) and TP (c) during operation period of different carbon source systems
Fig.3  Anaerobic phosphorus release (a) and phosphorus release rate curves (b) of different carbon source systems
Fig.4  Anoxic phosphorus absorption curve of different carbon source systems
sample ID reads OTUs coverage ACE Chao Shannon Simpson
Seed 1 30739 1353 0.993168 1475 1487 5.70 0.0106
Seed 2 30739 1396 0.992810 1529 1531 5.72 0.0113
Sy_A 30739 714 0.993884 889 886 4.35 0.0336
Sy_P 30739 530 0.994990 702 687 3.81 0.0784
Sy_AP 30739 525 0.995153 684 700 4.10 0.0354
Sy_G 30739 540 0.995413 684 679 4.31 0.0293
Tab.1  The community diversity under 3% cutoff divided OTU condition
Fig.5  The dominant “phylum” (a) and “genus” (b) in systems of phosphorus removal sludge with different carbon sources
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