The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the exogenous <i>Phragmites australis</i> and strain JS45

doi:10.1007/s11783-018-1016-0

Front. Environ. Sci. Eng.

2018, Vol. 12

Issue (1) : 11 https://doi.org/10.1007/s11783-018-1016-0

RESEARCH ARTICLE

The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the exogenous Phragmites australis and strain JS45

Xiangqun Chi^1,², Yingying Zhang¹, Daosheng Wang¹, Feihua Wang¹, Wei Liang¹(

)

¹. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
². BGI-Qing dao, Qingdao 266555, China

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Abstract

Nitrobenzene degraded rapidly and was removed completely in native sediments.

Indigenous microorganisms in native sediments are abundant.

Proteobacteria and Firmicutes might play important roles in nitrobenzene removal.

P. australis could provide a more suitable environment for Thauera.

The feasibility of using Phragmites australis-JS45 system in removing nitrobenzene from sediments was conducted. However, it was observed that nitrobenzene degraded rapidly and was removed completely within 20 days in native sediments, raising the possibility that indigenous microorganisms may play important roles in nitrobenzene degradation. Consequently, this study aimed to verify this possibility and investigate the potential nitrobenzene degraders among indigenous microorganisms in sediments. The abundance of inoculated strain JS45 and indigenous bacteria in sediments was quantified using real-time polymerase chain reaction. Furthermore, community structure of the indigenous bacteria was analyzed through high throughput sequencing based on Illumina MiSeq platform. The results showed that indigenous bacteria in native sediments were abundant, approximately 10¹⁴ CFU/g dry weight, which is about six orders of magnitude higher than that in fertile soils. In addition, the levels of indigenous Proteobacteria (Acinetobacter, Comamonadaceae_uncultured, Pseudomonas, and Thauera) and Firmicutes (Clostridium, Sporacetigenium, Fusibacter, Youngiibacter, and Trichococcus) increased significantly during nitrobenzene removal. Their quantities sharply decreased after nitrobenzene was removed completely, except for Pseudomonas and Thauera. Based on the results, it can be concluded that indigenous microorganisms including Proteobacteria and Firmicutes can have great potential for removing nitrobenzene from sediments. Although P. australis - JS45 system was set up in an attempt to eliminate nitrobenzene from sediments, and the system did not meet the expectation. The findings still provide valuable information on enhancing nitrobenzene removal by optimizing the sediment conditions for better growth of indigenous Proteobacteria and Firmicutes.

Keywords Community structure Indigenous microorganisms Nitrobenzene Plant-microbe associated remediation Sediment

Corresponding Author(s): Wei Liang

Issue Date: 07 December 2017

Cite this article:

Xiangqun Chi,Yingying Zhang,Daosheng Wang, et al. The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the exogenous Phragmites australis and strain JS45[J]. Front. Environ. Sci. Eng., 2018, 12(1): 11.

URL:

https://academic.hep.com.cn/fese/EN/10.1007/s11783-018-1016-0
https://academic.hep.com.cn/fese/EN/Y2018/V12/I1/11

Tab.1 Treatment setup

Tab.2 Primers and probe used in this study

Fig.1 Nitrobenzene removal and aniline accumulation among different microcosms. (a) Removal of nitrobenzene; (b) the variations of aniline concentrations. Values are means±SD (error bars) (n = 3) (NB: nitrobenzene; BA: aniline)

Fig.2 cnbA gene abundance variation in inoculated microcosms. Values are means±SD (error bars) (n = 3) (NB: nitrobenzene)

Fig.3 Phylotype rarefaction curve of each sample (97% level)

Fig.4 Shannon index of microbial diversity in each sample

Fig.5 Barplot of indigenous microbial community structure of samples at different times and in different microcosms

Fig.6 PCA analysis of microbial community structures in each sample

Fig.7 Heatmap analysis of indigenous microbial community in each sample

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