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Denitrification and phosphorus uptake by DPAOs using nitrite as an electron acceptor by step-feed strategies |
Bin MA1,2, Shuying WANG2, Guibing ZHU3, Shijian GE2, Junmin WANG2, Nanqi Ren1, Yongzhen PENG1,2() |
1. State Key Laboratory of Urban Water Resource and Environment (HIT), Harbin Institute of Technology, Harbin 150090, China; 2. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100022, China; 3. State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China |
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Abstract Denitrifying phosphorus accumulating organisms (DPAOs) using nitrite as an electron acceptor can reduce more energy. However, nitrite has been reported to have an inhibition on denitrifying phosphorus removal. In this study, the step-feed strategy was proposed to achieve low nitrite concentration, which can avoid or relieve nitrite inhibition. The results showed that denitrification rate, phosphorus uptake rate and the ratio of the phosphorus uptaken to nitrite denitrified (anoxic P/N ratio) increased when the nitrite concentration was 15 mg·L-1 after step-feeding nitrite. The maximum denitrification rate and phosphorus uptake rate was 12.73 mg NO2-–N·g MLSS-1?h-1 and 18.75 mg PO43-–P·g MLSS-1?h-1, respectively. These rates were higher than that using nitrate (15 mg·L-1) as an electron acceptor. The maximum anoxic P/N ratio was 1.55 mg PO43--P?mg NO2--N-1. When the nitrite concentration increased from 15 to 20 mg NO2--N?L-1 after addition of nitrite, the anoxic phosphorus uptake was inhibited by 64.85%, and the denitrification by DPAOs was inhibited by 61.25%. Denitrification rate by DPAOs decreased gradually when nitrite (about 20 mg·L-1) was added in the step-feed SBR. These results indicated that the step-feed strategy can be used to achieve denitrifying phosphorus removal using nitrite as an electron acceptor, and nitrite concentration should be maintained at low level (<15 mg·L-1 in this study).
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Keywords
denitrifying phosphate accumulating organisms (DPAOs)
denitrification
phosphorus uptake
nitrite
step-feed
enhanced biological phosphorus removal
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Corresponding Author(s):
PENG Yongzhen,Email:pyz@bjut.edu.cn
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Issue Date: 01 April 2013
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