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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front Envir Sci Eng    2013, Vol. 7 Issue (2) : 267-272    https://doi.org/10.1007/s11783-012-0439-2
RESEARCH ARTICLE
Denitrification and phosphorus uptake by DPAOs using nitrite as an electron acceptor by step-feed strategies
Bin MA1,2, Shuying WANG2, Guibing ZHU3, Shijian GE2, Junmin WANG2, Nanqi Ren1, Yongzhen PENG1,2()
1. State Key Laboratory of Urban Water Resource and Environment (HIT), Harbin Institute of Technology, Harbin 150090, China; 2. Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100022, China; 3. State Key Laboratory of Environmental Aquatic Quality, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
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Abstract

Denitrifying phosphorus accumulating organisms (DPAOs) using nitrite as an electron acceptor can reduce more energy. However, nitrite has been reported to have an inhibition on denitrifying phosphorus removal. In this study, the step-feed strategy was proposed to achieve low nitrite concentration, which can avoid or relieve nitrite inhibition. The results showed that denitrification rate, phosphorus uptake rate and the ratio of the phosphorus uptaken to nitrite denitrified (anoxic P/N ratio) increased when the nitrite concentration was 15 mg·L-1 after step-feeding nitrite. The maximum denitrification rate and phosphorus uptake rate was 12.73 mg NO2-N·g MLSS-1?h-1 and 18.75 mg PO43-–P·g MLSS-1?h-1, respectively. These rates were higher than that using nitrate (15 mg·L-1) as an electron acceptor. The maximum anoxic P/N ratio was 1.55 mg PO43--P?mg NO2--N-1. When the nitrite concentration increased from 15 to 20 mg NO2--N?L-1 after addition of nitrite, the anoxic phosphorus uptake was inhibited by 64.85%, and the denitrification by DPAOs was inhibited by 61.25%. Denitrification rate by DPAOs decreased gradually when nitrite (about 20 mg·L-1) was added in the step-feed SBR. These results indicated that the step-feed strategy can be used to achieve denitrifying phosphorus removal using nitrite as an electron acceptor, and nitrite concentration should be maintained at low level (<15 mg·L-1 in this study).

Keywords denitrifying phosphate accumulating organisms (DPAOs)      denitrification      phosphorus uptake      nitrite      step-feed      enhanced biological phosphorus removal     
Corresponding Author(s): PENG Yongzhen,Email:pyz@bjut.edu.cn   
Issue Date: 01 April 2013
 Cite this article:   
Bin MA,Shuying WANG,Guibing ZHU, et al. Denitrification and phosphorus uptake by DPAOs using nitrite as an electron acceptor by step-feed strategies[J]. Front Envir Sci Eng, 2013, 7(2): 267-272.
 URL:  
https://academic.hep.com.cn/fese/EN/10.1007/s11783-012-0439-2
https://academic.hep.com.cn/fese/EN/Y2013/V7/I2/267
Fig.1  Operation strategy of SBR. A: the nitrite concentration was about 15 mg·L after adding the nitrite solution; B: the nitrite concentration was about 20 mg·L after adding the nitrite solution
Initial nitrite concentration /(mg NO2--N?L-1)anoxic phosphorus uptake rate/(mg PO43-P?g MLSS-1?h-1)denitrification rate/(mg NO2--N?g MLSS-1?h-1)
10.532.033.80
15.821.572.36
21.630.021.56
Tab.1  Rates of anoxic phosphorus uptake and denitrification under different initial nitrite concentration in the batch tests
Fig.2  Denitrification rate after each addition of nitrite (15 mg·L) during the first stage
Fig.3  Anoxic phosphorus uptake rate after each addition of nitrite (15 mg·L) during the first stage
Fig.4  Anoxic P/N ratio after each addition of nitrite (15 mg·L) during the first stage
Fig.5  Denitrification time after the addition of nitrite (20 mg·L) during the second stage
Fig.6  Variations of pH in the typical cycle during the second stage
timeanoxic phosphorus uptake rate/(mg PO43--P?g MLSS-1?h-1)denitrification rate/(mg NO2--N?g MLSS-1?h-1)pH change rate/(10-3·min-1)
initial 60 min after the first addition3.753.594.6
middle 60 min after the first addition1.821.972.6
last 80 min after the first addition2.772.959.8
initial 120 min after the second addition6.494.1911
Tab.2  Denitrification rate, anoxic phosphorus uptake rate, and pH change rate in the typical cycle of the second stage. The nitrite concentration was 20 mg·L after each addition of nitrite.
1 Kuba T, van Loosdrecht M C M, Brandse F A, Heijnen J J. Occurrence of denitrifying phosphorus removing bacteria in modified UCT-type wastewater treatment plants. Water Research , 1997, 31(4): 777–786
doi: 10.1016/S0043-1354(96)00370-3
2 Ahn J, Daidou T, Tsuneda S, Hirata A. Characterization of denitrifying phosphate-accumulating organisms cultivated under different electron acceptor conditions using polymerase chain reaction-denaturing gradient gel electrophoresis assay. Water Research , 2002, 36(2): 403–412
doi: 10.1016/S0043-1354(01)00222-6 pmid:11827346
3 Hu Z R, Wentzel M C, Ekama-A. Anoxic growth of phosphate-accumulating organisms (PAOs) in biological nutrient removal activated sludge systems. Water Research , 2002, 36(19): 4927–4937
doi: 10.1016/S0043-1354(02)00186-0 pmid:12448537
4 Zeng R J, Saunders A M, Yuan Z G, Blackall L L, Keller J. Identification and comparison of aerobic and denitrifying polyphosphate-accumulating organisms. Biotechnology and Bioengineering , 2003, 83(2): 140–148
doi: 10.1002/bit.10652 pmid:12768619
5 Carvalho G, Lemos P C, Oehmen A, Reis M A M. Denitrifying phosphorus removal: linking the process performance with the microbial community structure. Water Research , 2007, 41(19): 4383–4396
doi: 10.1016/j.watres.2007.06.065 pmid:17669460
6 Zhou Y, Pijuan M, Yuan Z G. Free nitrous acid inhibition on anoxic phosphorus uptake and denitrification by poly-phosphate accumulating organisms. Biotechnology and Bioengineering , 2007, 98(4): 903–912
doi: 10.1002/bit.21458 pmid:17486651
7 Kuba T, VanLoosdrecht M C M, Heijnen J J. Phosphorus and nitrogen removal with minimal cod requirement by integration of denitrifying dephosphatation and nitrification in a two-sludge system. Water Research , 1996, 30(7): 1702–1710
doi: 10.1016/0043-1354(96)00050-4
8 Peng Y Z, Zhu-B. Biological nitrogen removal with nitrification and denitrification via nitrite pathway. Applied Microbiology and Biotechnology , 2006, 73(1): 15–26
doi: 10.1007/s00253-006-0534-z pmid:17028876
9 Meinhold J, Arnold E, Isaacs S. Effect of nitrite on anoxic phosphate uptake in biological phosphorus removal activated sludge. Water Research , 1999, 33(8): 1871–1883
doi: 10.1016/S0043-1354(98)00411-4
10 Lee D S, Jeon C O, Park J M. Biological nitrogen removal with enhanced phosphate uptake in a sequencing batch reactor using single sludge system. Water Research , 2001, 35(16): 3968–3976
doi: 10.1016/S0043-1354(01)00132-4 pmid:12230180
11 Ma B, Peng Y Z, Wang S Y, Ge S J, Yang Y Y, Zhu G B. Characterization of polyphosphate-accumulating bacteria community structure in enhanced biological phosphorus removal reactor. CIESC Journal , 2010, 61(5): 1282–1285 (in Chinese)
12 Yang Q, Peng Y Z, Liu X H, Zeng W, Mino T, Satoh H. Nitrogen removal via nitrite from municipal wastewater at low temperatures using real-time control to optimize nitrifying communities. Environmental Science &amp; Technology , 2007, 41(23): 8159–8164
doi: 10.1021/es070850f pmid:18186353
13 Smolders G J F, van der Meij J, van Loosdrecht M C M, Heijnen J J. Stoichiometric model of the aerobic metabolism of the biological phosphorus removal process. Biotechnology and Bioengineering , 1994, 44(7): 837–848
doi: 10.1002/bit.260440709 pmid:18618851
14 APHA. Standard methods for the examination of water and wastewater. 19th ed. Washington, DC: American Public Health Association/American Water Works Association/Water Envirionment Federation , 1995
15 Wang Y Y, Peng Y Z, Peng C Y, Wang S Y, Zeng W. Influence of ORP variation, carbon source and nitrate concentration on denitrifying phosphorus removal by DPB sludge from dephanox process. Water Science and Technology , 2004, 50(10): 153–161
pmid:15656308
16 Mancinelli R L, McKay C P. Effects of nitric oxide and nitrogen dioxide on bacterial growth. Applied and Environmental Microbiology , 1983, 46(1): 198–202
pmid:6351744
17 Zumft W G. Nitric oxide signaling and NO dependent transcriptional control in bacterial denitrification by members of the FNR-CRP regulator family. Journal of Molecular Microbiology and Biotechnology , 2002, 4(3): 277–286
pmid:11931559
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