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Frontiers of Environmental Science & Engineering

ISSN 2095-2201

ISSN 2095-221X(Online)

CN 10-1013/X

Postal Subscription Code 80-973

2018 Impact Factor: 3.883

Front Envir Sci Eng    2013, Vol. 7 Issue (4) : 539-551
Selective pseudosolubilization capability of Pseudomonas sp. DG17 on n-alkanes and uptake mechanisms analysis
Fei HUA, Hongqi WANG()
College of Water Sciences, Beijing Normal University, Beijing 100875, China
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Pseudosolubilized ability of Pseudomonas sp. DG17 on n-alkanes, role of biosurfactants in n-octadecane uptake and trans-membrane transport mechanism of n-octadecane were studied by analyzing amount of pseudosolubilized oil components in water phase, and the fraction of radiolabeled 14C n-octadecane in the broth and cell pellet. GC-MS results showed that pseudosolubilized oil components were mainly C12 to C28 of n-alkanes. In n-octadecane broth, pseudosolubilized n-octadecane could be accumulated as long as pseudosolubilized rate was faster than mineralization rate of substrate, and the maximum concentration of pseudosolubilized n-octadecane achieved to 45.37 mg·L-1. All of these results showed that Pseudomonas sp. DG17 mainly utilized alkanes by directly contacting with pseudosolubilized small oil droplets in the water phase. Analysis of 14C amount in cell pellet revealed that an energy-dependent system mainly controlled the trans-membrane transport of n-octadecane.

Keywords Pseudomonas      alkane      uptake      pseudosolubilization      trans-membrane transport     
Corresponding Authors: WANG Hongqi,   
Issue Date: 01 August 2013
 Cite this article:   
Fei HUA,Hongqi WANG. Selective pseudosolubilization capability of Pseudomonas sp. DG17 on n-alkanes and uptake mechanisms analysis[J]. Front Envir Sci Eng, 2013, 7(4): 539-551.
No.retention time /minnamemolecular formulaMWstruture
414.465naphthalene, 2,3,6-trimethylC13H14170
715.724heptadecane, 4-methylC18H38254
1117.880nonadecane, 2-methylC20H42282
1217.927erucic acidC22H42O2338
1418.8021-Hexadecanol, 2-methylC17H36O256
2123.8361,2-Benzenedicarboxylic acid, diisooctyl esterC24H38O4390
Tab.1  Pseudosolubilizd crude oil components in the water phase by sp. DG17
retention time /minmolecular formula20d35d
areatotal /%areatotal /%
9.603C12H261.059E+ 72.4681.118 E+ 71.286
12.415C14H301.241 E+ 73.0241.243 E+ 71.467
13.821C15 H321.074E+ 72.5041.526E+ 71.903
14.899C16H344.292E+ 60.8061.966E+ 72.452
16.062C17H369.865E+ 62.2266.658E+ 78.304
17.193C18H382.672E+ 60.7214.732E+ 75.902
18.171C19H401.240E+ 60.2913.023E+ 73.771
19.157C20 H422.924E+ 60.9223.675E+ 74.584
20.103C21 H444.490E+ 61.0523.868E+ 74.824
21.873C23 H486.746E+ 61.5813.817E+ 74.761
22.704C24H508.715E+ 62.0433.507E+ 74.374
23.506C25 H521.077E+ 72.5252.975E+ 73.711
24.273C26 H541.002E+ 72.3462.226E+ 72.776
25.015C27 H561.207E+ 72.8292.218E+ 72.766
25.816C28 H587.968E+ 62.1062.175E+ 72.713
26.740C29 H607.804E+ 61.9471.704E+ 72.125
28.023C30 H627.527 E+ 61.7461.778E+ 72.217
Tab.2  Pseudosolubilized -alkanes components in the water phase under the effect of sp. DG17
retention time /minmolecular formularesidue crude oil componentsfresh crude oil components
areatotal /%areatotal /%
9.606C12H269.221E+ 082.4811.501E+ 091.973
11.233C13H281.858E+ 094.3243.403E+ 094.463
12.408C14 H302.114E+ 094.9233.841E+ 095.043
13.690C15 H321.982E+ 094.6163.657E+ 094.798
14.899C16H342.219E+ 095.1763.327E+ 094.374
16.053C17H362.937E+ 096.8465.045E+ 096.614
17.136C18H382.219E+ 095.1212.960E+ 093.887
18.177C19H403.39E+ 097.9033.318E+ 094.351
19.164C20 H421.204E+ 092.7952.529E+ 093.315
20.110C21 H441.363E+ 093.1592.552E+ 093.348
21.876C23 H481.201E+ 092.8012.584E+ 093.392
22.709C24H501.168E+ 092.7312.357E+ 093.094
23.507C25 H528.827E+ 082.0582.109E+ 092.768
24.235C26 H548.254E+ 081.6831.701E+ 092.233
25.017C27 H568.084E+ 081.3911.284E+ 091.676
25.827C28 H587.763 E+ 081.0818.421 E+ 081.134
26.740C29 H607.534E+ 080.8217.307E+ 080.959
28.023C30 H627.521E+ 080.8146.958E+ 080.914
Tab.3  -alkanes components changes in oil film by sp. DG17
Fig.1  Cell growth of sp. DG17 on -octadecane (open triangle) and surface tension changes of culture medium (open circle)
Fig.2  Content distribution of -octadecane in the medium. Total -octadecane in the broth (open triangle); pseudosolubilization of -octadecane (filled triangle); Control group(open diamond)
Fig.3  Phase-contrast micrograph of oil droplets size in the culture medium (a: crude oil droplets in the water phase after incubation for 35d-40 magnification; b: -octadecane droplets in the water phase at 48h-40 magnification). Bar represents 0.02 mm
Fig.4  Morphological changes of sp. DG17 (a) Cells grown on crude oil at 35d-30000 magnification; (b) Cells grown on 400 mg·L of octadecane at 120h-20000 magnification). Bar represents 2 μm
Fig.5  C -octadecane biodegradation by sp. DG17 in the aqueous phase (a) and cellular C (b) of sp. DG17. C in control group (open squares); Cells in the absence of NaN (open triangles); Cells in the presence of NaN (open diamonds)
Fig.6  Effect of different inhibitors on the transport of C -octadecane by sp. DG17. Cells in the absence of inhibitor (open diamonds), cells treated with 0.1 mmol·LCCCP (open squares), and cells treated with 30 mmol·L NaN (open triangles)
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