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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2008, Vol. 2 Issue (1) : 63-69     DOI: 10.1007/s11684-008-0012-3
Construction of an immortalized neural progenitor cell strain and analysis of its immunogenicity
AN Ke1, XU Ying2, TIAN Xuebi3, GAO Feng3, TIAN Yuke3, YANG Hui3, ZHANG Chuanhan3
1.Department of Anesthesiology, The First Affiliated Hospital of Sun Yat-sen University; 2.Department of Anesthesiology, Children's Hospital of Chongqing Medical University; 3.Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
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Abstract  Neural progenitor cells (NPC) are those that are the source of neural cells for cell transplantation and gene therapy. The shortage in quantity and the limited life spans of primary cultured cells limit its widespread use in basic research. Immortalized NPC, which also possess the capacity of self-renewal and can proliferate infinitely, can produce a large number of NPCs with stable phenotype and genotype. Here we report that an immortalized neural progenitor cell strain, which we named as INPC, was successfully established by gene-transfer of simian virus 40 large T antigen gene mediated by liposomes. The INPC retained the biological characteristics of its original cells and provided a safe and reliable cell platform for the treatment of central nervous system diseases and transgenic cell transplantation. INPC could express low levels of MHC antigens which was down-regulated after differentiation. This indicates that INPC possesses poor immunogenicity. The immortalized cells may show good long-term survival and do not elicit an acute immunological response following transplantation.
Issue Date: 05 March 2008
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