Please wait a minute...
Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2008, Vol. 2 Issue (1) : 75-81     DOI: 10.1007/s11684-008-0014-1
Developmental expression of CAPON and Dexras1 in spinal cord of rats
LI Xin1, CHENG Chun2, CHEN Mengling2, GAO Shangfeng2, ZHAO Jian3, NIU Shuqiong4, SHEN Aiguo4
1.The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University; Department of Microbiology and Immunology, Nantong University; 2.Department of Microbiology and Immunology, Nantong University; 3.Department of Orthopaedics, Affiliated Hospital of Nantong University; 4.The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University;
Download: PDF(272 KB)   HTML
Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract  To study the expression of the carboxy-terminal PSD-95/DLG/ZO-1 ligand of nNOS (CAPON) and Dexras1 mRNA during development in the spinal cord of rats, real-time polymerase chain reaction (Real-time PCR), as a quantitative method, was used to study the developmental expression of CAPON and Dexras1 mRNA level in the spinal cord. The spatial expression of CAPON and Dexras1 mRNA was examined by a combination of in situ hybridization (ISH) and immunofluorescence. During the development of the spinal cord, CAPON mRNA was expressed in low levels from embryo day 14 to day 18. At postnatal day 1, it reached the peak and was expressed in the part which will become the dorsal horn when mature. It then decreased gradually until postnatal week 12, when it presented in the ventral horn. At embryo day 14, Dexras1 mRNA was expressed at low levels, increased during embryo day 16 to day 18 and peaked at postnatal day 1. Spatiotemporal expression of Dexras1 mRNA was similar to CAPON as confirmed by correlation analysis and colocalization. CAPON and neuronal nitric oxide synthase (nNOS) was expressed within the same cells of the dorsal horn at postnatal day 1 but had different subcellular localizations. Co-expression of CAPON and Dexras1 mRNA in myeloid tissue during development process of rat indicates that the adaptor protein, CAPON may play a probable role in differentiation of neurons, synaptic plasticity and synaptogenesis by regulating nNOS to activate Dexras1.
Issue Date: 05 March 2008
URL:     OR
1 Jaffrey S R Snowman A M Eliasson M J Cohen N A Snyder S H CAPON: a protein associated with neuronalnitric oxide synthase that regulates its interactions with PSD95Neuron 1998 20(1)115124
2 Fang M Jaffrey S R Sawa A Ye K Luo X Snyder S H Dexras1: aG protein specifically coupled to neuronal nitric oxide synthase viaCAPONNeuron 2000 28(1)183193
3 Kemppainen R J Behrend E N Dexamethasone rapidly inducesa novel Ras superfamily member-related gene in AtT-20 cellsJ Biol Chem 1998 273(6)31293131
4 Gruner J A Amonitored contusion model of spinal cord injury in the ratJ Neurot 1992 9(2)123126
5 Jaffrey S R Benfenati F Snowman A M Czernik A J Snyder S H Neuronal nitric-oxide synthase localizationmediated by a ternary complex with synapsin and CAPONProc Natl Acad Sci USA 2002 99(5)31993204
6 Che Y H Tamatani M Tohyama M Changes in mRNA for post-synaptic density-95 (PSD-95) andcarboxy-terminal PDZ ligand of neuronal nitric oxide synthase followingfacial nerve transectionBrain Res Mol BrainRes 2000 76(2)325335
7 Ségalat L Grisoni K Archer J Vargas C Bertrand A Anderson J E CAPONexpression in skeletal muscle is regulated by position, repair, NOSactivity, and dystrophyExp Cell Res 2005 302(2)170179
8 Brzustowicz L M Simone J Mohseni P Hayter J E Hodgkinson K A Chow E W Bassett A S Linkage disequilibriummapping of schizophrenia susceptibility to the CAPON region of chromosome1q22Am J Hum Genet 2004 74(5)10571063
9 Zheng Y Li H Qin W Chen W Duan Y Xiao Y Li C Zhang J Li X Feng G He L Associationof the carboxyl-terminal PDZ ligand of neuronal nitric oxide synthasegene with schizophrenia in the Chinese Han populationBiochem Biophys Res Commun 2005 328(4)809815
10 Xu B Wratten N Charych E I Buyske S Firestein B L Brzustowicz L M Increased expression in dorsolateral prefrontal cortexof CAPON in schizophrenia and bipolar disorderPLoS Med 2005 2(10)e263
11 Inglis F M Furia F Zuckerman K E Strittmatter S M Kalb R G The role of nitric oxide andNMDA receptors in the development of motor neuron dendritesJ Neurosci 1998 18(24)1049310501
12 Virgili M Monti B LoRusso A Bentivogli M Contestabile A Developmental effects of invivo and in vitro inhibition of nitric oxide synthase in neuronsBrain Res 1999 839(1)164172
13 Sánchez-Islas E León-Olea M Nitric oxide synthase inhibitionduring synaptic maturation decreases synapsin I immunoreactivity inrat brainNitric Oxide 2004 10(3)141149
14 Cismowski M J Takesono A Ma C Lizano J S Xie X Fuernkranz H Lanier S M Duzic E Genetic screens in yeast to identify mammalian nonreceptormodulators of G-protein signalingNat Biotechnol 1999 17(9)878883
15 Cheah J H Kim S F Hester L D Clancy K W Patterson S E Papadopoulos V Snyder S H NMDA receptor-nitricoxide transmission mediates neuronal iron homeostasis via the GTPaseDexras1Neuron 2006 51(4)431440
16 Graham T E Prossnitz E R Dorin R I Dexras1/AGS-1 inhibits signal transduction from the Gi-coupledformyl peptide receptor to Erk-1/2 MAP kinasesJ Biol Chem 2002 277(13)1087610882
17 Graham T E Qiao Z Dorin R I Dexras1 inhibits adenylyl cyclaseBiochem Biophys Res Commun 2004 316(2)307312
18 Takesono A Nowak M W Cismowski M Duzic E Lanier S M Activator of G-protein signaling 1 blocksGIRK channel activation by a G-protein-coupled receptor: apparentdisruption of receptor signaling complexesJ Biol Chem 2002 277(16)1382713830
19 Nguyen C H Watts V J Dexras1 blocks receptor-mediatedheterologous sensitization of adenylyl cyclase 1Biochem Biophys Res Commun 2005 332(3)913920
20 Cismowski M J Ma C Ribas C Xie X Spruyt M Lizano J S Lanier S M Duzic E Activation of heterotrimeric G-protein signaling by a Ras-relatedproteinJ Biol Chem 2000 275(31)2342123424
21 Nguyen C H Watts V J Dexamethasone-induced Ras protein1 negative regulate protein kinase C delta: implication for adenylylcyclase 2 signalingMol Pharmocal 2006 69(5)17631771
22 Shen A Chen M Niu S Sun L Gao S Shi S Li X Lv Q Guo Z Cheng C Changes in mRNAfor CAPON and Dexras1 in adult rat following sciatic nerve transactionJ Chem Neuroanat 2007 [Epub ahead of print]
23 Liu B Y Yuan Z R Xiao W Guo X S Yuan L Nuclear translocation of neuronal nitricoxide svnthaseShandong Daxue Xuebao 2005 4310631068 (in Chinese)
No related articles found!
Full text