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Analyses of levels of thyroid hormones and its
receptor expression in puerperants and newborns from an e-waste dismantling
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| JU Ying1, CHEN Lan1, JIANG Qi1, YANG Kedi1, CHEN Xuemin1, XU Guojian2, LI Liping2 |
| 1.Department of Occupational and Environmental Health and the MOE Key Lab of Environment and Health, School of Public Health, Huazhong University of Science & Technology; 2.Research Center for Injury Prevention, Shantou University Medical College; |
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Abstract In this study, the serum levels, including thyroid hormones free triiodothyronine (FT3), free thyroxine(FT4),thyroid stimulating hormone(TSH)among the subjects from the exposed group (n = 48) and the control group (n = 45) were detected by immuno radiometric assay (IRMA). The expression levels of TR?1, TR?1, TSHR mRNA in placentas and umbilical cords were detected by fluorescent quantitative real-time PCR (FQ-PCR). The correlations between the thyroid hormone levels in maternal serum and umbilical serum, and between the expression levels of its receptors mRNA in placentas and umbilical cords were determined. We found that the FT4 levels of both maternal serum and umbilical cord serum in the exposed group were lower than those in the control (P < 0.05). However, the increased TSH levels in the exposed group had statistically significance compared to those in the control group (P < 0.05). The TR?1 and TR?1 mRNA levels both in placentas and umbilical cords in the exposed group were lower than those in the control group (P < 0.05 and 0.01). However, the TSHR mRNA levels in the exposed group were significantly different compared to those in the control group (P < 0.01). The serum FT4 and TSH levels of parturient women were positively correlated with those of the newborns in both groups (P < 0.05 and 0.01). The mRNA levels of TR?1, TR?1 and TSHR in the placentas were positively correlated with those in umbilical cords in both groups (P < 0.01). The findings suggest that some environmental pollutants existing in the electronic waste (e-waste) dismantling region may affect the health of local parturient women and newborns, representing changes both in serum levels of thyroid hormones and in mRNA expression of its receptors.
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Issue Date: 05 September 2008
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