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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2009, Vol. 3 Issue (1) : 108-112     DOI: 10.1007/s11684-009-0015-8
RESEARCH ARTICLE |
Celecoxib in combination with retinoid CD437 inhibits melanoma A375 cell in vitro
Jianwen REN, Zhenhui PENG(), Birong GUO, Min PAN
Department of Dermatology, the Second Hospital of Xi’an Jiaotong University, Xi’an 710004, China
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Abstract  

This study aimed to investigate the effects of celecoxib, synthetic retinoid 6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-naphthalenecarboxylic acid (CD437) and the combination of the two on cell proliferation, apoptosis, and cycle arrest of human malignant melanoma A375 cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazoliumbromide assay (MTT assay) was applied to determine the anti-proliferative effects of the drugs on human malignant melanoma A375 cells. Flow cytometry was performed to investigate the influence of the drugs on cell cycle and cell apoptosis. Both celecoxib and CD437 could inhibit the growth of human malignant melanoma A375 cells in a dose-dependent manner. Celecoxib at 80 μmol/L inhibited proliferation, induced apoptosis and G2/M cell cycle arrest of human malignant melanoma A375 cells after treatment for 24 h [proliferation inhibiting rate: (50.2±2.51)%, apoptosis rate: (35.91±1.80)%]. CD437 at 10 μmol/L inhibited proliferation, induced apoptosis and G0/G1 cell cycle arrest of human malignant melanoma A375 cells after treatment for 24 h [proliferation inhibiting rate: (58.6±2.38)%, apoptosis rate: (28.03±0.77)%]. Celecoxib in combination with CD437 could significantly enhance the effects of inhibiting proliferation and inducing apoptosis of human malignant melanoma A375 cells 24 h after treatment compared with the drug alone [proliferation inhibiting rate: (68.92±1.72)%, apoptosis rate: (42.09±1.05)%, both P<0.05] and could decrease the proportion of the S phase in the cell cycle. Celecoxib could inhibit the growth of human malignant melanoma A375 cells by inducing apoptosis and G2/M cycle arrest. CD437 could inhibit the growth of human malignant melanoma A375 cells by inducing apoptosis and G0/G1 cycle arrest. Celecoxib exhibited additive effects with CD437 on retarding the growth and inducing apoptosis of human malignant melanoma A375 cells. Celecoxib in combination with CD437 may become an effective method for prevention and treatment of human melanoma.

Keywords celecoxib      CD437      melanoma A375 cell      apoptosis      cycle arrest     
Corresponding Authors: PENG Zhenhui,Email:pengzhenhui2006@126.com   
Issue Date: 05 March 2009
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0015-8     OR     http://academic.hep.com.cn/fmd/EN/Y2009/V3/I1/108
Fig.1  PIR on A375 cells by different concentrations of celecoxib. PIR: proliferation inhibiting ratio.
Fig.2  PIR on A375 cells by different concentrations of CD437. PIR: proliferation inhibiting ratio.
Fig.3  PIR on A375 cells treated by 80 μmol/L celecoxib in combination with 10 μmol/L CD437. PIR: proliferation inhibiting ratio.
Fig.4  A375 cell apoptosis signal map after drug treatment for 24 h. FITC: fluorescein isothiocyanate; PI: propidium iodide.
Fig.5  A375 cell cycle arrest signal map after drug treatment for 24 h
1 Meric J B, Rottey S, Olaussen K, Soria J C, Khayat D, Rixe O, Spano J P. Cyclooxygenase-2 as a target for anticancer drug development. Crit Rev Oncol Hematol , 2006, 59(1): 51-64
doi: 10.1016/j.critrevonc.2006.01.003
2 Patrignani P, Tacconelli S, Sciulli M G, Capone M L. New insights into COX-2 biology and inhibition. Brain Res Brain Res Rev , 2005, 48(2): 352-359
doi: 10.1016/j.brainresrev.2004.12.024
3 Yamada N, Ohira M, Hirakawa K. COX and study of cancer therapy. Gan To Kagaku Ryoho , 2004, 31(8): 1147-1151
4 Futagami S, Suzuki K, Hiratsuka T, Shindo T, Hamamoto T, Ueki N, Kusunoki M, Miyake K, Gudis K, Tsukui T, Sakamoto C. Chemopreventive effect of celecoxib in gastric cancer. Inflammopharmacology , 2007, 15(1): 1-4
doi: 10.1007/s10787-006-1541-5
5 Amir M, Agarwal H K. Role of COX-2 selective inhibitors for prevention and treatment of cancer. Pharmazie , 2005, 60(8): 563-570
6 Johnson G E, Ivanov V N, Hei T K. Radiosensitization of melanoma cells through combined inhibition of protein regulators of cell survival. Apoptosis , 2008, 13(6): 790-802
doi: 10.1007/s10495-008-0212-y
7 Chiu L C, Tong K F, Ooi V E. Cytostatic and cytotoxic effects of cyclooxygenase inhibitors and their synergy with docosahexaenoic acid on the growth of human skin melanoma A-375 cells. Biomed Pharmacother , 2005, 59(Suppl 2): S293-S297
doi: 10.1016/S0753-3322(05)80049-6
8 Grosch S, Maier T J, Schiffmann S, Geisslinger G. Cyclooxygenase-2 (COX-2)-independent anticarcinogenic effects of selective COX-2 inhibitors. J Natl Cancer Inst , 2006, 98(11): 736-747
9 Farhana L, Dawson M I, Leid M, Wang L, Moore D D, Liu G, Xia Z, Fontana J A. Adamantyl-substituted retinoid-related molecules bind small heterodimer partner and modulate the Sin3A repressor. Cancer Res , 2007, 67(1): 318-325
doi: 10.1158/0008-5472.CAN-06-2164
10 Garattini E, Parrella E, Diomede L, Gianni M, Kalac Y, Merlini L, Simoni D, Zanier R, Ferrara F F, Chiarucci I, Carminati P, Terao M, Pisano C. ST1926, a novel and orally active retinoid-related molecule inducing apoptosis in myeloid leukemia cells: modulation of intracellular calcium homeostasis. Blood , 2004, 103(1): 194-207
doi: 10.1182/blood-2003-05-1577
11 Zhao X, Demary K, Wong L, Vaziri C, McKenzie A B, Eberlein T J, Spanjaard R A. Retinoic acid receptor-independent mechanism of apoptosis of melanoma cells by the retinoid CD437 (AHPN). Cell Death Differ , 2001, 8(9): 878-886
doi: 10.1038/sj.cdd.4400894
12 Danielsson C, Torma H, Vahlquist A, Carlberg C. Positive and negative interaction of 1,25-dihydroxyvitamin D3 and the retinoid CD437 in the induction of human melanoma cell apoptosis. Int J Cancer , 1999, 81(3): 467-470
doi: 10.1002/(SICI)1097-0215(19990505)81:3&lt;467::AID-IJC22&gt;3.0.CO;2-2
13 Zhang X K. Targeting Nur77 translocation. Expert Opin Ther Targets , 2007, 11(1): 69-79
doi: 10.1517/14728222.11.1.69
14 Zou C, Zhou J, Qian L, Feugang J M, Liu J, Wang X, Wu S, Ding H, Zou C, Liebert M, Grossman H B. Comparing the effect of ATRA, 4-HPR, and CD437 in bladder cancer cells. Front Biosci , 2006, 11: 2007-2016
doi: 10.2741/1942
15 Kim H J, Lotan R. Identification of protein modulation by the synthetic retinoid CD437 in lung carcinoma cells using high throughput immunoblotting. Int J Oncol , 2005, 26(2): 483-491
16 Solary E, Bettaieb A, Dubrez-Daloz L, Corcos L. Mitochondria as a target for inducing death of malignant hematopoietic cells. Leuk Lymphoma , 2003, 44(4): 563-574
doi: 10.1080/1042819021000038001
17 Rishi A K, Zhang L, Boyanapalli M, Wali A, Mohammad R M, Yu Y, Fontana J A, Hatfield J S, Dawson M I, Majumdar A P, Reichert U. Identification and characterization of a cell cycle and apoptosis regulatory protein-1 as a novel mediator of apoptosis signaling by retinoid CD437. J Biol Chem , 2003, 278(35): 33422-33435
doi: 10.1074/jbc.M303173200
18 Watanabe Y, Tsuchiya H, Sakabe T, Matsuoka S, Akechi Y, Fujimoto Y, Yamane K, Ikeda R, Nishio R, Terabayashi K, Ishii K, Gonda K, Matsumi Y, Ashla A A, Okamoto H, Takubo K, Tomita A, Hoshikawa Y, Kurimasa A, Itamochi H, Harada T, Terakawa N, Shiota G. CD437 induces apoptosis in ovarian adenocarcinoma cells via ER stress signaling. Biochem Biophys Res Commun , 2008, 366(3): 840-847
doi: 10.1016/j.bbrc.2007.12.028
19 Jin F, Liu X, Zhou Z, Yue P, Lotan R, Khuri F R, Chung L W, Sun S Y. Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells. Cancer Res , 2005, 65(14): 6354-6363
doi: 10.1158/0008-5472.CAN-04-4061
20 Kadara H, Schroeder C P, Lotan D, Pisano C, Lotan R. Induction of GDF-15/NAG-1/MIC-1 in human lung carcinoma cells by retinoid-related molecules and assessment of its role in apoptosis. Cancer Biol Ther , 2006, 5(5): 518-522
21 Holmes W F, Soprano D R, Soprano K J. Synthetic retinoids as inducers of apoptosis in ovarian carcinoma cell lines. J Cell Physiol , 2004, 199(3): 317-329
doi: 10.1002/jcp.10338
22 Zhao X, Spanjaard R A. The apoptotic action of the retinoid CD437/AHPN: diverse effects, common basis. J Biomed Sci , 2003, 10(1): 44-49
doi: 10.1007/BF02255996
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