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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2009, Vol. 3 Issue (1) : 36-40     DOI: 10.1007/s11684-009-0020-y
Influence of β-elemene on the secretion of angiotensin II and expression of AT1R in hepatic stellate cells
Ling YANG1, Rui ZHU2, Qingjing ZHU3, Dan DAN1, Jin YE1(), Keshu XU1, Xiaohua HOU1
1. Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China; 2. Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China; 3. Department of Integrated Traditional Chinese Medicine and Western Medicine, Wuhan Municipal Hospital of Infectious Diseases, Wuhan 430022, China
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This study aims to investigate the influence of β-elemene on the secretion of angiotensin II (ANG II) and the expression of angiotensin receptor type 1 (AT1R) in hepatic stellate cells (HSCs). In vitro, HSC-T6 were cultured for 24 hours and then treated with different doses of β-elemene (2.5, 5 and 10 mg/L). A control group was also set up. The secretion of ANG II in the supernatant was detected by radioimmunoassay. The mRNA expression of AT1R at 4, 12 and 24 h after treatment was detected by reverse transcription-polymerase chain reaction (RT-PCR), respectively. The protein expression of AT1R was detected by western blot. At the 4th h, the ANG II secretion in the supernatant was significantly inhibited by 10 mg/L β-elemene compared with the control group (P<0.05), while 5.0 mg/L and 2.5 mg/L β-elemene had no inhibitory effect on the secretion of ANG II (P>0.05). At the time point of the 12th h, the secretion of ANG II in the supernatant treated with 10 mg/L and 5.0 mg/L β-elemene was significantly lower than the control (P<0.01, P<0.05). Following the treatment with 5.0 mg/L and 2.5 mg/L β-elemene for 24 h, significant inhibition of ANG II secretion was observed (P<0.05), but 10 mg/L β-elemene had no such effect. β-elemene significantly reduced the amount of AT1R mRNA in HSCs after the treatment for 4, 12, and 24 h in a dose-dependent manner. The expression of AT1R protein also decreased after the treatment with β-elemene for 24 h. β-elemene can inhibit the secretion of ANG II and the gene and protein expression of AT1R, which may be the mechanism by which β-elemene prevents the progress of hepatic fibrosis.

Keywords liver cirrhosis      beta-elemene      hepatic stellate cells      angiotensin II      receptor, angiotensin, type 1     
Corresponding Authors: YE Jin,   
Issue Date: 05 March 2009
URL:     OR
group4t h h12t h h24 th h
10.0 mg/L β-elemene50.970±8.08183.727±6.850**106.420±3.912
5.0 mg/L β-elemene58.650±9.25991.090±3.226*104.133±3.296*
2.5 mg/L β-elemene61.020±8.77594.607±3.548100.957±2.581**
Tab.1  Inhibitory effect of β-elemene on the secretion of ANG II in HSCs
Fig.1  β-elemene inhibited the expression of AT1R mRNA in hepatic stellate cells. (a) HSC-T6 were incubated in DMEM with 10% FBS. After incubation for 24 h, β-elemene of the indicated concentration was added to the cells and incubation was continued for the indicated time. AT1R mRNA was determined by RT-PCR. (b) AT1R protein in HSCs following treatment with 10, 5.0 and 2.5 mg/L β-elemene for 24 h was detected by western blot. Values are presented as ( = 3 for each experimental group). Three independent experiments were performed (: <0.001 control cells). Lane 1: 10 mg/L β-elemene; Lane 2: 5.0 mg/L β-elemene; Lane 3: 2.5 mg/L β-elemene.
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