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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2009, Vol. 3 Issue (2) : 136-140     DOI: 10.1007/s11684-009-0029-2
RESEARCH ARTICLE |
Effects of resistin on insulin signaling in endothelial cells
Zhizhen LI1, Fangping LI2, Jianhong YE2, Li YAN2, Zuzhi FU2()
1. Department of Endocrinology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China; 2. Department of Endocrinology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China
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Abstract  

The objective of this study was to investigate the effects of resistin on insulin signaling in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with recombinant human resistin (0-100 ng/mL) for 24 h. Akt and endothelial nitric oxide synthase (eNOS) phosphorylation levels of endothelial cells under basal or insulin stimulated conditions were measured by Western blot. Nitric oxide (NO) production of HUVECs was also detected. The results showed that resistin could significantly inhibit Akt and eNOS phosphorylation and NO production in endothelial cells under insulin stimulated conditions (P< 0.05 vs control). But under basal conditions, treatment with resistin could result in a decrease in eNOS phosphorylation (P< 0.05 vs control) but had no effect on NO production and Akt phosphorylation levels. These findings suggested that resistin exerted an inhibitory effect on NO production by inhibiting insulin signaling and eNOS phosphorylation in endothelial cells.

Keywords resistin      endothelium      nitric oxide      endothelial nitric oxide synthase      Akt-binding protein, mouse     
Corresponding Authors: FU Zuzhi,Email:lzz940537@163.com   
Issue Date: 05 June 2009
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0029-2     OR     http://academic.hep.com.cn/fmd/EN/Y2009/V3/I2/136
groupscontrol cells15 ng/mL resistin50 ng/mL resistin100 ng/mL resistin
without insulin5.97 ± 1.655.47 ± 0.365.45 ± 1.955.71 ± 0.67
insulin stimulated4.89 ± 0.584.01 ± 0.69*3.76 ± 0.71*3.73 ± 0.45*
Tab.1  NO production levels of each group
Fig.1  Akt phosphorylation level in human umbilical vein endothelial cells without insulin stimulation: a representative Western blot of Akt phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.
Fig.2  Insulin stimulated Akt phosphorylation level in human umbilical vein endothelial cells: a representative Western blot of Akt phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.
Fig.3  eNOS phosphorylation level in human umbilical vein endothelial cells without insulin stimulation: a representative Western blot of eNOS phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.
Fig.4  Insulin stimulated eNOS phosphorylation level in human umbilical vein endothelial cells: a representative Western blot of eNOS phosphorylation. 1: control group; 2: res15 group; 3: res50 group; 4: res100 group.
groupsp-Aktp-eNOS
without insulininsulin stimulatedwithout insulininsulin stimulated
control cells0.67 ± 0.161.14 ± 0.221.48 ± 0.130.17 ± 0.02
15 ng/mL resistin0.61 ± 0.130.79 ± 0.19*1.02 ± 0.110.12 ± 0.03*
50 ng/mL resistin0.53 ± 0.150.68 ± 0.10*1.01 ± 0.090.13 ± 0.34*
100 ng/mL resistin0.49 ± 0.160.60 ± 0.07*0.92 ± 0.130.13 ± 0.03*
Tab.2  Akt and eNOS phosphorylation levels of each group
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