|
|
Expression and clinical implication of PRL-1 and PRL-3 in transitional cell carcinoma of bladder |
Bin HAO1,2(), Changwei LIU2, Huixiang LI3 |
1. Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2. The Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014, China; 3. The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450003, China |
|
|
Abstract The mRNA and protein expression of phosphatase of regenerating liver 1 (PRL-1) and phosphatase of regenerating liver 3 (PRL-3) in transitional cell carcinoma of bladder (BTCC) and normal epithelia of bladder was investigated, and the relationship between the BTCC and pathological changes was clarified. The expression of PRL-1 and PRL-3 mRNA was detected by using reverse transcription polymerase chain reaction (RT-PCR) in 30 cases of BTCC and 10 cases of normal bladder, and the expression of PRL-1 and PRL-3 protein was checked by using immunohistochemistry in 30 cases of BTCC and 15 cases of normal bladder. The expression levels of PRL-1 and PRL-3 mRNA and protein were higher in BTCC than those in normal bladder epithelia (P<0.05). The increased expression of PRL-1 and PRL-3 mRNA and protein was detectable in deep invasion and metastasis of BTCC (P<0.05). There was no correlation between the expression of PRL-1 and PRL-3 and gender, age or recurrence of BTCC (all P>0.05). A significantly positive correlation was found between PRL-1 and PRL-3 in BTCC (P<0.05). PRL-1 and PRL-3 are expressed consistently and may contribute to the growth, differentiation, invasion and metastasis of BTCC.
|
Keywords
transitional cell carcinoma of bladder
phosphatase of regenerating liver 1
phosphatase of regenerating liver 3
reverse transcription polymerase chain reaction
immunohistochemistry
|
Corresponding Author(s):
HAO Bin,Email:binhao68@hotmail.com
|
Issue Date: 05 June 2009
|
|
1 |
Hunter T. Signaling — 2000 and beyond. Cell , 2000, 100(1): 113-127
|
2 |
Lyon M A, Ducruet A P, Wipf P, Lazo J S. Dual-specificity phosphatases as targets for antineoplastic agents. Nat Rev Drug Discov , 2002, 1(12): 961-976 doi: 10.1038/nrd963
|
3 |
Zeng Q, Si X, Horstmann H, Xu Y, Hong W, Pallen C J. Prenylation dependent association of protein-tyrosine phosphatases PRL-1, -2, and-3 with the plasma membrane and the early endosome. J Biol Chem , 2000, 275(28): 21444-21452 doi: 10.1074/jbc.M000453200
|
4 |
Diamond R H, Cressman D E, Laz T M, Abrams C S, Taub R. PRL-1, a unique nuclear protein tyrosine phosphatase, affects cell growth. Mol Cell Biol , 1994, 14(6): 3752-3762
|
5 |
Zeng Q, Dong J M, Guo K, Li J, Koh V, Pallen C J, Manser E, Hong W. PRL-3 and PRL-1 promote cell migration, invasion, and metastasis. Cancer Res , 2003, 63(11): 2716-2722
|
6 |
Wang Q, Holmes D I, Powell S M, Lu Q L, Waxman J. Analysis of stromal-epithelial interactions in prostate cancer identifies PTPCAAX2 as a potential oncogene. Cancer Lett , 2002, 175(1): 63-69 doi: 10.1016/S0304-3835(01)00703-0
|
7 |
Saha S, Bardelli A, Buckhaults P, Velculescu V E, Rago C, St Croix B, Romans K E, Choti M A, Lengauer C, Kinzler K W, Vogelstein B. A phosphatase associated with metastasis of colorectal cancer. Science , 2001, 294(5545): 1343-1346 doi: 10.1126/science.1065817
|
8 |
Miskad U A, Semba S, Kato H, Yokozaki H. Expression of PRL-3 phosphatase in human gastric carcinomas: close correlation with invasion and metastasis. Pathobiology , 2004, 71(4): 176-184 doi: 10.1159/000078671
|
9 |
Radke I, G?tte M, Kersting C, Mattsson B, Kiesel L, Wülfing P. Expression and prognostic impact of the protein tyrosine phophatases PRL-1, PRL-2 and PRL-3 in breast cancer. Brit J Cancer , 2006, 95(3): 347-354 doi: 10.1038/sj.bjc.6603261
|
10 |
Zhang Z Y, Zhou B, Xie L. Modulation of protein kinase signaling by protein phosphatases and inhibitor. Pharmacol Ther , 2002, 93(2,3): 307-317
|
11 |
Dumaual C M, Sandusky G E, Crowell P L, Randall S K. Cellular localization of PRL-1 and PRL-2 gene expression in normal adult human tissues. J Histochem Cytochem , 2006, 54(12): 1401-1412 doi: 10.1369/jhc.6A7019.2006
|
12 |
Matter W F, Estridge T, Zhang C, Belagaje R, Stancato L, Dixon J, Johnson B, Bloem L, Pickard T, Donaqhue M, Acton S, Jeyaseelan R, Kadambi V, Vlahos C J. Role of PRL-3, a human muscle-specific tyrosine phosphatase, in angiotensin-II signaling. Biochem Biophys Res Commun , 2001, 283(5): 1061-1068 doi: 10.1006/bbrc.2001.4881
|
13 |
Cates C A, Michael R L, Stayrook K R, Harvey K A, Burke Y D, Randall S K, Crowell P L, Crowell D N. Prenylation of oncogenic human PTP (CAAX) protein tyrosine phosphatases. Cancer Lett , 1996, 110(1,2): 49-55
|
14 |
Miskad U A, Semba S, Kato H, Matsukawa Y, Kodama Y, Mizuuchi E, Maeda N, Yanagihara K, Yokozaki H. High PRL-3 expression in human gastric cancer is a marker of metastasis and grades of malignancies: an in situ hybridization study. Virchows Arch . 2007, 450(3): 303-310 doi: 10.1007/s00428-006-0361-8
|
15 |
Polato F, Codegoni A, Fruscio R, Perego P, Manqioni C, Saha S, Bardelli A, Broqqini M. PRL-3 phosphatase is implicated in ovarian cancer growth. Clin Cancer Res , 2005, 11(9 Pt 1): 6835-6839 doi: 10.1158/1078-0432.CCR-04-2357
|
16 |
Wu X, Zeng H, Zhang X, Zhao Y, Sha H, Ge X, Zhang M, Gao X, Xu Q. Phosphatase of regenerating liver-3 promotes motility and metastasis of mouse melanoma cells. Am J Pathol , 2004, 164(6): 2039-2054
|
17 |
Peng L, Ning J, Meng L, Shou C. The association of the expression level of protein tyrosine phosphatase PRL-3 protein with liver metastasis and prognosis of patients with colorectal cancer. J Cancer Res Clin Oncol , 2004, 130(9): 521-526 doi: 10.1007/s00432-004-0563-x
|
18 |
Bardelli A, Saha S, Sager J A, Romans K E, Xin B, Markowitz S D, Lengauer C, Velculescu V E, Kinzler K W, Voqelstein B. PRL-3 expression in metastatic cancers. Clin Cancer Res , 2003, 9(15): 5607-5615
|
19 |
Guo K, Li J, Tang J P, Koh V, Gan B Q, Zeng Q. Catalytic domain of PRL-3 plays an essential role in tumor metastasis: formation of PRL-3 tumors inside the blood vessels. Cancer Biol Ther , 2004, 3(10): 945-951
|
20 |
Sager J, Benvenuti S, Bardelli A. PRL-3: a phosphatase for metastasis? Cancer Biol Ther , 2004, 3(10): 952-953
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|