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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2009, Vol. 3 Issue (3) : 357-362     DOI: 10.1007/s11684-009-0046-1
Research articles |
Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113
Yumei DING BM , Lili CHEN MD , Bo CHENG PhD , Handong ZHANG MM ,
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;
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Abstract  The purpose of this study was to investigate the expression of pituitary tumor transforming gene (PTTG) and basic fibroblast growth factor (bFGF) in oral squamous cell carcinoma (OSCC) and tongue cancer cell line Tca8113, as well as their effects on each other. We detected PTTG protein and bFGF in OSCC tissues from 56 cases using the streptavidin-biotin peroxidase (S-P) method; additionally, after being treated with different concentrations of anti bFGF or PTTG antibody, PTTG or bFGF expression in Tca8113 was examined by immunocytochemistry. The results were as follows: (1) Positive rates of PTTG protein and bFGF were 78.2% and 67.3% in OSCC, respectively, which were significantly higher than those in normal mucosal tissues (P<0.05). PTTG protein was significantly up-regulated in poorly and moderately differentiated tumors compared to well differentiated tumors (P<0.05), and there was also a significant difference between tumors with lymph node metastasis and tumors without lymph node metastasis (P<0.05). PTTG protein expression was positively correlated with bFGF (r = 0.382, P<0.05); (2) PTTG protein emitted strong fluorescence in Tca8113, and it decreased after being treated with anti-bFGF antibody. Anti-PTTG antibody also had an inhibitive effect on bFGF expression. In summary, the overexpression of PTTG protein is closely related with OSCC differentiation and lymph node metastasis. PTTG protein expression conforms to bFGF in OSCC tissues and Tca8113 cells. Detection of both PTTG and bFGF may help to judge the degree of malignancy and prognosis of patients with OSCC.
Keywords carcinoma, squamous cell      pituitary tumor transforming gene (PTTG) protein       basic fibroblast growth factor      
Issue Date: 05 September 2009
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0046-1     OR     http://academic.hep.com.cn/fmd/EN/Y2009/V3/I3/357
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