|
|
Effect of decoction on CD14 expression in lipopolysaccharide signal transduction
pathway of alcohol-induced liver disease in rats |
Rui ZHU MD 1, Lin SHEN MD 1, Jianguo LIU MD 1, Weili ZHANG MM 1, Ling YANG MD 2, |
1.Department of Traditional
Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong
University of Science and Technology, Wuhan 430022, China; 2.Department of Gastroenterology,
Union Hospital, Tongji Medical College, Huazhong University of Science
and Technology, Wuhan 430022, China; |
|
|
Abstract This paper aims to investigate the effects of Zhihuang (枳黄) decoction on CD14 expression in the lipopolysaccharide signal transduction pathway of alcohol-induced liver disease in rats. Seventy-five Wistar rats were randomly divided into three groups. Ethanol (56%, weight/volumn) was intragastrically administrated to 50 rats (14mL/kg body weight per day) for 10 days to establish a model of alcohol-induced liver disease, and 25 of these 50 rats were treated with Zhihuang decoction simultaneously. Liver injury was evaluated by biochemical examination. The plasma content of endotoxin was assayed by biochemistry. The expression of CD14 mRNA and protein in rat liver was measured by reverse transcriptional polymerase chain reaction and immunohistochemistry, respectively. Zhihuang decoction pretreatment significantly protected against acute alcohol-induced liver injury, which was evidenced by the decrease of elevated serum alanine aminotransferase and aspartate aminotransferase. In addition, the level of plasma endotoxin and up-regulation of CD14 was also suppressed by Zhihuang decoction in alcohol-intoxicated rats. Zhihuang decoction can significantly reduce CD14 expression in the lipopolysaccharide signal transduction pathway, which is one of the most important mechanisms of Zhihuang decoction to treat hepatic injury induced by alcohol in rats.
|
Keywords
liver disease
alcohol
Zhihuang decoction
CD14
signal transduction
|
Issue Date: 05 September 2009
|
|
|
Zhao J, Chen H, Li Y. Protective effect of bicyclol on acute alcohol-inducedliver injury in mice. Eur J Pharmacol, 2008, 586(1): 322―331
doi: 10.1016/j.ejphar.2008.02.059
|
|
Moriarty K J, Platt H, Crompton S, Darling W, Blakemore M, Hutchinson S, Proctor D, Brown M, Burtun B, Lipscomb G, Padmakumar K. Collaborative care for alcoholrelated liver disease. Clin Med, 2007, 7(2): 125―128
|
|
Zhu R, Yang L, Shen L, Ye J, Liu J G, Hu S J. ANG II-AT1 receptor pathway is involved in the anti-fibrotic effectof β-elemene. J Huazhong Univ SciTechnol [Med Sci], 2009, 29(2): 177―181
|
|
Stern M, Carter E A, Walker W A. Food proteins and gut mucosal barrier. IV. Effects ofacute and chronic ethanol administration on handling and uptake ofbovine serum albumin by rat small intestine. Dig Dis Sci, 1986, 31(11): 1242―1248
doi: 10.1007/BF01296527
|
|
Parlesak A, Schäfer C, Schütz T, Bode JC, Bode C. Increased intestinal permeability tomacromolecules and endotoxemia in patients with chronic alcohol abusein different stages of alcohol-induced liver disease. J Hepatol, 2000, 32(5): 742―747
doi: 10.1016/S0168-8278(00)80242-1
|
|
Fukui H, Brauner B, Bode J C, Bode C. Plasma endotoxinconcentrations in patients with alcoholic and non-alcoholic liverdisease: reevaluation with an improved chromogenic assay. J Hepatol, 1991, 12(2): 162―169
doi: 10.1016/0168-8278(91)90933-3
|
|
Bode C, Kugler V, Bode J C. Endotoxemia in patients with alcoholic and non-alcoholiccirrhosis and in subjects with no evidence of chronic liver diseasefollowing acute alcohol excess. J Hepatol, 1987, 4(11): 8―14
doi: 10.1016/S0168-8278(87)80003-X
|
|
Nanji A A, Khettry U, Sadrzadeh S M, Yamanaka T. Severityof liver injury in experimental alcoholic liver disease: correlationwith plasma endotoxin, prostaglandin E2, leukotriene B4 and thromboxaneB2. Am J Pathol, 1993, 142(11): 367―373
|
|
Mathison J C, Ulevitch R J. The clearance, tissue distribution,and cellular localization of intravenously injected lipopolysaccharidein rabbits. J Immunol, 1979, 123(5): 2133―2143
|
|
Zlydaszyk J C, Moon R J. Fate of 51Cr-labeled lipopolysaccharide in tissue culture cellsand livers of normal mice. Infect Immun, 1976, 14(1): 100―105
|
|
Bigatello L M, Broitman S A, Fattori L, Di Paoli M, Pontello M, Bevilacqua G, Nespoli A. Endotoxemia,encephalopathy, and mortality in cirrhotic patients. Am J Gastroenterol, 1987, 82(1): 11―15
|
|
Lin R S, Lee F Y, Lee S D, Tsai Y T, Lin H C, Lu R H, Hsu W C, Huang C C, Wang S S, Lo K J. Endotoxemia in patients with chronicliver diseases: relationship to severity of liver diseases, presenceof esophageal varices and hyperdynamic circulaton. J Hepatol, 1995, 22(2): 165―172
doi: 10.1016/0168-8278(95)80424-2
|
|
Nolan J P. The role of endotoxin in liver injury. Gastroenterology, 1975, 69(6): 1346―1356
|
|
Nolan J P, Camara D S. Intestinal endotoxins asco-factorsin liver injury. Immunol Invest, 1989, 18(1―4): 325―337
doi: 10.3109/08820138909112246
|
|
Urbaschek R, McCuskey R S, Rudi V, Becker K P, Stickel F, Urbaschek B, Seitz H K. Endotoxin,endotoxin-neutral- lizing-capacity, sCD14, sICAM-1, and cytokinesin patients with various degrees of alcoholic liver disease. Alcohol Clin Exp Res, 2001, 25(2): 261―268
doi: 10.1111/j.1530-0277.2001.tb02207.x
|
|
Kitano H, Fukui H, Okamoto Y, Kikuchi E, Matsumoto M, Kikukawa M, Morimura M, Tsujita S, Nagamoto I, Nakatani T, Takaya A, Tsujii T. Role of albumin and high-density lipoprotein as endotoxin-bindingproteins in rats with acute and chronic alcohol loading. Alcohol Clin Exp Res, 1996, 20(1): 73―76
doi: 10.1111/j.1530-0277.1996.tb01735.x
|
|
Beutler B. TNF,immunity and inflammatory disease: lessons of the past decade. J Inv Med, 1995, 43(3): 227―235
|
|
Jacob C O. Tumor necrosis factor-α in autoimmunity: pretty girl or oldwitch? Immunol Today, 1992, 13(1): 122―125
doi: 10.1016/0167-5699(92)90107-I
|
|
Järveläinen H A, Orpana A, Perola M, Savolainen V T, Karhunen P J, Lindros K O. Promoter polymorphism of the CD14 endotoxin receptorgene as a risk factor for alcoholic liver disease. Hepatology, 2001, 33(1): 1148―1153
doi: 10.1053/jhep.2001.24236
|
|
Landmann R, Knopf H P, Link S, Sansano S, Schumann R, Zimmerli W. Human monocyte CD14 is upregulated by lipopolysaccharide. Infect Immun, 1996, 64(5): 1762―1769
|
|
Paton A. Alcoholin the body. BMJ, 2005, 330(7482): 85―87
doi: 10.1136/bmj.330.7482.85
|
|
Yin M, Ikejima K, Wheeler M D, Bradford B U, Seabra V, Forman D T, Sato N, Thurman R G. Estrogen is involved in earlyalcohol-induced liver injury in a rat enteral feeding model. Hepatology, 2000, 31(1): 117―123
doi: 10.1002/hep.510310119
|
|
Dai L L, Gong J P, Zuo G Q, Wu C X, Shi Y J, Li X H, Peng Y, Deng W, Li S W, Liu C A. Synthesis of endotoxin receptor CD14protein in Kupffer cells and its role in alcohol-induced liver disease. World J Gastroenterol, 2003, 9(3): 622―626
|
|
Yin M, Bradford B U, Wheeler M D, Uesugi T, Froh M, Goyert S M, Thurman R G. Reduced early alcohol-induced liver injury in CD14-deficient mice. J Immunol, 2001, 166(7): 4737―4742
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
|
Shared |
|
|
|
|
|
Discussed |
|
|
|
|