Please wait a minute...
Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2009, Vol. 3 Issue (4) : 475-479    https://doi.org/10.1007/s11684-009-0086-6
Research articles
Expression of STAT6 and NF-κB p65 in the colon mucosa of patients with ulcerative colitis
Rui ZHU MD,Heng FAN MD,Lin SHEN MD,Jianguo LIU BD,Jia ZHAO MM,
Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;
 Download: PDF(150 KB)  
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract The expression of signal transducer and activator of transcription 6 (STAT6) and nuclear factor-κB (NF-κB) in the colonic mucosa of patients with ulcerative colitis (UC) was examined. Real-time polymerase chain reaction and immunohistochemistry were used to detect the expression of STAT6 and NF-κB p65 at both mRNA and protein levels in the colonic mucosa of patients with UC and healthy volunteers. The results showed that the expression levels of STAT6 and NFκB p65 in the colonic mucosa of patients with UC were significantly higher than in normal controls at both mRNA and protein levels. These data suggest that STAT6 and NFκB p65 perhaps play an important role in the pathogenesis of UC and underscore the potential value of anti-UC strategies in the clinical management of this disease.
Keywords ulcerative colitis      signal transducer and activator of transcription 6 (STAT6)      nuclear factor-κB p65 (NF-κB p65)      
Issue Date: 05 December 2009
 Cite this article:   
Jia ZHAO MM,Rui ZHU MD,Lin SHEN MD, et al. Expression of STAT6 and NF-κB p65 in the colon mucosa of patients with ulcerative colitis[J]. Front. Med., 2009, 3(4): 475-479.
 URL:  
https://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0086-6
https://academic.hep.com.cn/fmd/EN/Y2009/V3/I4/475
Lampinen M, Backman M, Winqvist O, Rorsman F, Rönnblom A, Sangfelt P, Carlson M. Differentregulation of eosinophil activity in Crohn’s disease comparedwith ulcerative colitis. J Leukoc Biol, 2008, 84(6): 1392―1399

doi: 10.1189/jlb.0807513
Lakatos L, Lakatos P L. Is the incidence and prevalenceof inflammatory bowel diseases increasing in Eastern Europe? Postgrad Med J, 2006, 82(967): 332―337

doi: 10.1136/pgmj.2005.042416
Shteingart S, Rapoport M, Grodzovski I, Sabag O, Lichtenstein M, Eavri R, Lorberboum-Galski H. Therapeuticpotency of IL2―caspase 3 targeted treatment in a murine experimentalmodel of inflammatory bowel disease. Gut, 2009, 58(6): 790―798

doi: 10.1136/gut.2008.153981
Trinatafillidis J K, Nasioulas G, Kosmidis P A. Colorectal cancer and inflammatory bowel disease: Epidemiology,risk factors, mechanisms of carcinogenesis and prevention strategies. Anticancer Res, 2009, 29(7): 2727―2737
Knight P, Campbell B J, Rhodes J M. Host-bacteria interaction in inflammatory bowel disease. Br Med Bull, 2008, 88(1): 95―113

doi: 10.1093/bmb/ldn038
Atreya I, Atreya R, Neurath M F. NF-kappaB in inflammatory bowel disease. J Intern Med, 2008, 263(6): 591―596

doi: 10.1111/j.1365-2796.2008.01953.x
Han W, Joo M, Everhart M B, Christman J W, Yull F E, Blackwell T S. Myeloid cells control termination of lung inflammationthrough the NF-kappaB pathway. Am J PhysiolLung Cell Mol Physiol, 2009, 296(3): 320―327

doi: 10.1152/ajplung.90485.2008
Cheng D S, Han W, Chen S M, Sherrill T P, Chont M, Park G Y, Sheller J R, Polosukhin V V, Christman J W, Yull F E, Blackwell T S. Airway epithelium controlslung inflammation and injury through the NF-kappaB Pathway. J Immunol, 2007, 178(10): 6504―6513
Alcamo E, Mizgerd J P, Horwitz B H, Bronson R, Beg A A, Scott M, Doerschuk C M, Hynes R O, Baltimore D. Targeted mutation of TNF receptor I rescues the RelA-deficientmouse and reveals a critical role for NF-κB in leukocyte recruitment. J Immunol, 2001, 167(3): 1592―1600
Wright J G, Christman J W. The role of nuclear factorkappa B in the pathogenesis of pulmonary diseases: implications fortherapy. Am J Respir Med, 2003, 2(3): 211―219
Lee H S, Moon C, Lee H W, Park E M, Cho M S, Kang J L. Src tyrosine kinases mediate activations of NF-κb and integrinsignal during lipopolysaccharide-induced acute lung injury. J Immunol, 2007, 179(10): 7001―7011
Sen P, Wallet M A, Yi Z, Huang Y, Henderson M, Mathews C E, Earp H S, Matsushima G, Baldwin A S Jr, Tisch R M. Apoptoticcells induce Mer tyrosine kinase-dependent blockade of NF-kappaB activationin dendritic cells. Blood, 2007, 109(2): 653―660

doi: 10.1182/blood-2006-04-017368
Hayden M S, Ghosh S. Shared principles in NF-kappaBsignaling. Cell, 2008, 132(3): 344―362

doi: 10.1016/j.cell.2008.01.020
Kato T Jr, Delhase M, Hoffmann A, Karin M. CK2 Is a C-TerminalIkappaB Kinase Responsible for NF-kappaB Activation during the UVResponse. Mol Cell, 2003, 12(4): 829―839

doi: 10.1016/S1097-2765(03)00358-7
Tergaonkar V, Bottero V, Ikawa M, Li Q, Verma I M. IkappaB kinase-independent IkappaBalphadegradation pathway: functional NF-kappaB activity and implicationsfor cancer therapy. Mol Cell Biol, 2003, 23(22): 8070―8083

doi: 10.1128/MCB.23.22.8070-8083.2003
Baldwin A S Jr. The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol, 1996, 14: 649―683

doi: 10.1146/annurev.immunol.14.1.649
Jones M R, Simms B T, Lupa M M, Kogan M S, Mizgerd J P. Lung NF-kappaB activationand neutrophil recruitment require IL-1 and TNF receptor signalingduring pneumococcal pneumonia. J Immunol, 2005, 175(11): 7530―7535
Sweeney C, Li L, Shanmugam R, Bhat-Nakshatri P, Jayaprakasan V, Baldridge L A, Gardner T, Smith M, Nakshatri H, Cheng L. Nuclear factor-kappaBis constitutively activated in prostate cancer in vitro and is overexpressedin prostatic intraepithelial neoplasia and adenocarcinoma of the prostate. Clin Cancer Res, 2004, 10(16): 5501―5507

doi: 10.1158/1078-0432.CCR-0571-03
Karin M, Cao Y, Greten F R, Li Z W. NF-kappaB in cancer: from innocent bystander to major culprit. Nat Rev Cancer, 2002, 2(4): 301―310

doi: 10.1038/nrc780
Caamaño J, Hunter C A. NF-kappaB family of transcriptionfactors: central regulators of innate and adaptive immune functions. Clin Microbiol Rev, 2002, 15(3): 414―429

doi: 10.1128/CMR.15.3.414-429.2002
Li Q, Verma I M. NF-kappaB regulation in theimmune system. Nat Rev Immunol, 2002, 2(10): 725―734

doi: 10.1038/nri910
Senftleben U, Li Z W, Baud V, Karin M. IKKbeta isessential for protecting T cells from TNFalpha-induced apoptosis. Immunity, 2001, 14(3): 217―230

doi: 10.1016/S1074-7613(01)00104-2
Mora A, Youn J, Keegan A, Boothby M. NF-kappaB/Rel participation in the lymphokine-dependent proliferation of Tlymphoid cells. J Immunol, 2001, 166(4): 2218―2227
Hettmann T, DiDonato J, Karin M, Leiden J M. An essential role for nuclear factor kappaB in promoting doublepositive thymocyte apoptosis. J Exp Med, 1999, 189(1): 145―158

doi: 10.1084/jem.189.1.145
Tak P P, Firestein G S. NF-kappaB: a key role ininflammatory diseases. J Clin Invest, 2001, 107(1): 7―11

doi: 10.1172/JCI11830
Perkins N D. The Rel/NF-kappaB family: friend and foe. Trends Biochem Sci, 2000, 25(9): 434―440

doi: 10.1016/S0968-0004(00)01617-0
Lu H, Ouyang W, Huang C. Inflammation, a key event in cancer development. Mol Cancer Res, 2006, 4(4): 221―233

doi: 10.1158/1541-7786.MCR-05-0261
Neurath M F, Fuss I, Schürmann G, Pettersson S, Arnold K, Müller-Lobeck H, Strober W, Herfarth C, Büschenfelde K H. Cytokine gene transcription by NF-kappaB family members in patients with inflammatory bowel disease. Ann N Y Acad Sci, 1998, 859: 149―159

doi: 10.1111/j.1749-6632.1998.tb11119.x
Li Z, Zhang de K, Yi W Q, Ouyang Q, Chen Y Q, Gan H T. NF-kappaB p65 antisense oligonucleotides may serve asa novel molecular approach for the treatment of patients with ulcerativecolitis. Arch Med Res, 2008, 39(8): 729―734

doi: 10.1016/j.arcmed.2008.08.001
Yang J, Stark G R. Roles of unphosphorylatedSTATs in signaling. Cell Res, 2008, 18(4): 443―451

doi: 10.1038/cr.2008.41
Darnell J E Jr. STATs and gene regulation. Science, 1997, 277(5332): 1630―1635

doi: 10.1126/science.277.5332.1630
Mudter J, Weigmann B, Bartsch B, Kiesslich R, Strand D, Galle P R, Lehr H A, Schmidt J, Neurath M F. Activation pattern of signal transducers and activators of transcription(STAT) factors in inflammatory bowel diseases. Am J Gastroenterol, 2005, 100(1): 64―72

doi: 10.1111/j.1572-0241.2005.40615.x
Hebenstreit D, Wirnsberger G, Horejs-Hoeck J, Duschl A. Signalingmechanisms, interaction partners, and target genes of STAT6. Cytokine Growth Factor Rev, 2006, 17(3): 173―188

doi: 10.1016/j.cytogfr.2006.01.004
Schindler C, Levy D E, Decker T. JAK-STAT signaling: from interferons to cytokines. J Biol Chem, 2007, 282(28): 20059―20063

doi: 10.1074/jbc.R700016200
[1] Hang Xiang,Xiaomei Zhang,Chao Yang,Wenhuan Xu,Xin Ge,Rong Zhang,Ya Qiu,Wanjun Sun,Fan Li,Tianyuan Xiang,Haixu Chen,Zheng Wang,Qiang Zeng. Autologous bone marrow stem cell transplantation for the treatment of ulcerative colitis complicated with herpes zoster: a case report[J]. Front. Med., 2016, 10(4): 522-526.
[2] Heng FAN MD, Jia ZHAO MM, Lin SHEN BM, Qing TANG MD, Zhexin SHOU MM, Li LIANG BM, Yi LIAO BM, Xiaoyan CHEN BM, . Effect of compound Sophorae Flavescentis Jiechangrong capsule on expression of NF-κB p65 and STAT6 in the intestinal mucosa of patients with ulcerative colitis[J]. Front. Med., 2009, 3(4): 480-484.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed