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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2010, Vol. 4 Issue (2) : 225-228     DOI: 10.1007/s11684-010-0003-z
Research articles |
Effect of bradykinin on bradykinin-B2 receptor in rat aortic vascular smooth muscle cells and the involved signal transduction pathways
Wen YAN MD,Min FENG MD,Pei-Hua WANG MD,Dao-Wen WANG MD,
Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;
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Abstract  The aim of this paper is to study the effect of bradykinin (BK) on bradykinin-B2 receptor as well as the possible involved signal transduction pathways in cultured rat aortic vascular smooth muscle cells (VSMCs). Rat aortic VSMCs were cultured. Cells after 4–6 passages were used in the experiment. VSMCs were incubated with BK, BK+ B2 receptor inhibitor (HOE-140), BK+ MEK inhibitor (PD98059), BK+ mitogen-activated protein kinase (MAPK) inhibitor (apigenin), BK+ phosphoinositide 3-kinase (PI3K) inhibitor (LY294002), and BK+ Akt inhibitor to evaluate the expression of B2 receptor and phosphorylation of signaling molecules MAPK, Akt, and PI3K by Western blot. (1) BK markedly up-regulated the expression of B2 receptor in VSMC. (2) Apigenin, PD98059, Akt inhibitor, and LY294002 inhibited up-regulation of B2 receptor induced by BK. (3) Signal transduction pathways of MAPK and PI3K were involved in the up-regulation of B2 receptor by BK mediation. Results suggest that bradykinin can up-regulate the expression of B2 receptor in VSMCs.
Keywords bradykinin      vascular smooth muscle cells      signal transduction pathways      
Issue Date: 05 June 2010
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-010-0003-z     OR     http://academic.hep.com.cn/fmd/EN/Y2010/V4/I2/225
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