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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2010, Vol. 4 Issue (1) : 117-126     DOI: 10.1007/s11684-010-0011-z
Research articles |
Molecular epidemiology of Cryptococcus neoformans species complex isolates from HIV-positive and HIV-negative patients in southeast China
Min CHEN MM1,Wei-Hua PAN MD1,Wan-Qing LIAO MM1,Xiao-Ran LI BS2,Bi-Wei FENG BS2,Zhe-Xue QUAN PhD2,Shao-Xi WU MD3,Xiao-Ping TANG MD4,Zhi-Rong YAO MD5,
1.National Laboratory of Cryptococcus Neoformans, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; 2.Department of Microbiology & Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China; 3.Chinese Cultural Collection Commission for Microbiology, Institute of Dermatology, Chinese Academy of Medical Science, Nanjing 210042, China; 4.Guangzhou No.8 People’s Hospital, Guangzhou 510060, China; 5.Department of Dermatology, Xinhua Hospital, Shanghai Jiao Tong University, Shanghai 200092, China;
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Abstract  This study investigated the molecular types of the Cryptococcus neoformans species complex isolates and their clinical manifestations among human immunodeficiency virus (HIV)-positive and HIV-negative patients in southeast China in the past 15 years. The molecular types of 109 isolates from 108 patients were analyzed by the PCR fingerprinting method, sequences of internal transcribed spacers of rDNA (ITS region), and sequences of the capsule-associated gene (CAP59). In HIV-positive patients, clinical isolates were grouped into molecular types VNI (75%, 15/20), VNII (15%, 3/20), and VNIII (10%, 2/20). In HIV-negative patients, the majority of the clinical isolates were grouped into molecular types VNI (72%, 64/89), VNII (13%, 12/89), VGI (12%, 11/89), VNIII (1%, 1/89), and VGII (1%, 1/89). In reference to the mating type of the isolates, 97% (106/109) were of the MATα, 2% (2/109) were of the MATα/- and 1% (1/109) were of the MATα/a. As for the clinical manifestations of the molecular types among the patients, the average cerebrospinal fluid (CSF) pressure of the patients infected by the C. gattii was higher than that of the patients infected by the C. neoformans. These results suggest that both HIV-positive and HIV-negative cryptococcal patients in the southeast of China are mostly infected by the C. neoformans strains. No C. gattii strains were found in HIV-positive patients.
Keywords Cryptococcus neoformans      Cryptococcus gattii      cryptococcosis      molecular epidemiology      molecular type      mating type      
Issue Date: 05 March 2010
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