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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2010, Vol. 4 Issue (1) : 59-66     DOI: 10.1007/s11684-010-0015-8
Research articles |
Genetic variants in the ADD1 and GNB3 genes and blood pressure response to potassium supplementation
Dai-Hai YU PhD1,Jian-Feng HUANG MD1,Ji-Chun CHEN MS1,Jie CAO MS1,Shu-Feng CHEN PhD1,Dong-Feng GU MD, PhD, for the GenSalt Collaborative Research Group1,De-Pei LIU PhD2,Lai-Yuan WANG PhD3,Jing CHEN MD, MSc4,Jiang HE MD, PhD4,Cashell E. JAQUISH PhD5,Dabeeru C. RAO PhD6,Charles GU PhD6,James E. HIXSON PhD7,Chung-Shiuan CHEN MS8,Paul K. WHELTON MD, MSc9,
1.Department of Evidence-based Medicine and Population Genetics, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China; 2.Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China; 3.Department of Evidence-based Medicine and Population Genetics, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China;Chinese National Human Genome Center at Beijing, Beijing 100176, China; 4.Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA 70112, USA;Department of Medicine, Tulane University School of Medicine, New Orleans, LA 70112, USA; 5.National Heart, Lung, and Blood Institute, National Institute of Health, Bethesda, MD 20892, USA; 6.Washington University in St. Louis School of Medicine, St. Louis, MO 63110, USA; 7.University of Texas School of Public Health, Houston, TX 77030, USA; 8.Department of Epidemiology, Tulane University School of Public Health and Tropical Medicine, New Orleans, LA 70112, USA; 9.Loyola University Medical Center, Maywood, IL 60153, USA;
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Abstract  Dietary potassium-supplementation has been associated with a decreased risk of hypertension and other cardiovascular outcomes. However, blood pressure (BP) responses to potassium supplementation vary among individuals. This study was designed to examine the association between 12 single nucleotide polymorphisms (SNPs) in the adducin 1 alpha (ADD1) and guanine nucleotide binding protein (G protein) beta polypeptide 3 (GNB3) genes and systolic BP (SBP), diastolic BP (DBP), and mean arterial pressure (MAP) responses to potassium-supplementation. We conducted a 7-day high-sodium intervention (307.8 mmol sodium/day) followed by a 7-day high-sodium with potassium-supplementation (60 mmol potassium/day) among 1906 Han Chinese participants from rural north China. BP measurements were obtained at the end of each intervention period using a random-zero sphygmomanometer. We identified significant associations between ADD1 variant rs17833172 and SBP, DBP, and MAP responses to potassium-supplementation (all P<0.0001) that remained significant after adjustment for multiple comparisons. In participants that were heterozygous or homozygous for the G allele of this marker, SBP, DBP, and MAP response to potassium-supplementation were −3.52 (−3.82, −3.21), −1.41 (−1.66, −1.15) and −2.12 (−2.37, −1.87), respectively, as compared to the corresponding responses of 1.99 (0.25, 3.73), −0.65 (−0.10, −0.21), and −0.23 (−0.37, 0.83), respectively, for those who were homozygous for A allele. In addition, participants with at least one copy of the G allele of rs12503220 of the ADD1 gene had significantly increased DBP and MAP response to potassium-supplementation (P = 0.0041 and 0.01, respectively), which was also significant after correction for multiple testing. DBP and MAP responses to potassium-supplementation were −1.36 (−1.63, −1.10) and −2.07 (−2.32, −1.82) for those with at least G allele compared to corresponding responses of 0.86 (−0.68, 2.40) and −0.45 (−1.74, 0.84) for those who were homozygous for A allele. In summary, our study identified novel associations between genetic variants of the ADD1 gene and BP response to potassium-supplementation, which could have important clinical and public health implications. Future studies aimed at replicating these novel findings are warranted.
Keywords blood pressure      genetics      polymorphism      die-tary potassium      potassium sensitivity      adducin 1 alpha (ADD1)      guanine nucleotide binding protein beta polypeptide 3 (GNB3)      
Issue Date: 05 March 2010
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