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Inhibition of TNF-alpha secretion from peripheral
blood monocular cells by triptolid is associated with TNF-alpha-308
gene polymorphisms in rheumatoid arthritis patients |
| Xiu-Liang TAO MM,Sheng-Hao TU MD,Ri-Bo XIONG MM,Yong-Hong HU BM, |
| Department of Integrated
Traditional Chinese and Western Medicine, Tongji Hospital, Tongji
Medical College, Huazhong University of Science and Technology, Wuhan
430030, China; |
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Abstract This study examined the inhibitory effect of triptolid (TP) on tumor necrosis factor-α (TNF-α) secreted from peripheral blood monocular cells (PBMCs) and the association of the inhibitory effect with TNF-α-308 gene polymorphisms in rheumatoid arthritis (RA) patients. Gene polymorphism at A-G site 308 in the promoter region of TNF-α gene was detected in 42 RA patients by using allele specific polymerase chain reaction (AS-PCR) assay. PBMCs were harvested from these patients and treated first with lipopolysaccharides (LPS) and then with different doses of TP (1, 5.4 and 15 ng/mL). The TNF-α level in the supernatants was measured by enzyme-linked immunosorbent assay (ELISA). The results showed that TNF-α level in the supernatants of TP (1 ng/mL)-treated PBMCs was decreased by 3.80% and 4.91%, respectively, in the patients with AA and AG genotypes, when compared with those treated with LPS alone (P>0.05). Moreover, the TNF-α level in the patients with GG genotype was reduced by 20.74% (P<0.05). When PBMCs were treated with TP at 5.4 ng/mL, TNF-α levels in the patients with AA, AG, and GG genotypes were decreased by 20.42%, 34.73%, and 41.69%, respectively (P<0.05). The TNF-α level was slightly higher in the PBMCs treated with 15 ng/mL of TP than those in the two TP groups in the patients carrying AA, AG, and GG genotypes (P>0.05). It was concluded that gene polymorphism at TNF-α-308 sites may relate to the secretion of TNF-α in RA patients. TP has different inhibitory effects on the secretion of TNF-α in the patients harboring different genotypes, which may be one of the reasons for individual variation in response to TP.
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| Keywords
arthritis, rheumatoid
molonuclear cells
tumor necrosis factor
gene polymorphisms
triptolid
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Issue Date: 05 June 2010
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Christodoulou C, Choy E H. Joint inflammationand cytokine inhibition in rheumatoid arthritis. Clin Exp Med, 2006, 6(1): 13―19
doi: 10.1007/s10238-006-0088-5
|
|
Lotito A P, Silva C A, Mello S B. Interleukin-18 in chronic joint diseases. Autoimmun Rev, 2007, 6(4): 253―256
doi: 10.1016/j.autrev.2006.08.010
|
|
Kim J, Remick D G. Tumor necrosis factor inhibitors for the treatment of asthma. Curr Allergy Asthma Rep, 2007, 7(2): 151―156
doi: 10.1007/s11882-007-0013-3
|
|
Cipriano C, Caruso C, Lio D, Giacconi R, Malavolta M, Muti E, Gasparini N, Franceschi C, Mocchegiani E. The-308G/A polymorphism ofTNF-alpha influences immunological parameters in old subjects affectedby infectious diseases. Int J Immunogenet, 2005, 32(1): 13―18
doi: 10.1111/j.1744-313X.2005.00490.x
|
|
Yen J H, Chen C J, Tsai W C, Lin C H, Ou T T, Wu C C, Liu H W. Tumor necrosisfactor promoter polymorphisms in patients with rheumatoid arthritisin Taiwan. J Rheumatol, 2001, 28(8): 1788―1792
|
|
Warzocha K, Ribeiro P, Bienvenu J, Roy P, Charlot C, Rigal D, Coiffier B, Salles G. Geneticpolymorphisms in the tumor necrosis factor locus influence non-Hodgkin's lymphoma outcome. Blood, 1998, 91(10): 3574―3581
|
|
Zhu Y Y, Ye D F, Zhuo G S, Zhang S L, Lin J A, Liang X H. A study on TNF-α gene polymorphism in RA patientsand its significance. Zhong Guo Yi ShiZa Zhi, 2004, 6(5): 618―619 (in Chinese)
|
|
Culverhouse R C, Suarez B K, Beckmann L, Chen P, Chen Y S, Chiu Y F, Chang-Claude J, Dempfle A, Hein R, Kazma R, Lebrec J J, Lee S, Lim S, Maher B S, Park T, Perdry H, Wang K S, Wolkow P P, Xu W. Gene by environment interactions. Genet Epidemiol, 2007, 31Suppl 1: S68―74
doi: 10.1002/gepi.20282
|
|
Chen H B, Tu S H, Hu Y H. Current progress of single nucleotidepolymorphism in rheumatoid arthritis. ZhongHua Feng Shi Bing Xue Za Zhi, 2004, 8(3): 171―173 (in Chinese)
|
|
Kodar L. The pivotal role of TNF-a in reumatoid arthritis. Zhong Yi Yao Xue Kan, 2004, 22(10): 1847―1849 (in Chinese)
|
|
Yang L, Shi W, Wang J, Jiang X, Feng W, Li Z. Comparison of cell deaths induced by transmembrane andsecretory TNF-alpha. J Huazhong Univ SciTechnolog Med Sci, 2007, 27(2): 117―119
doi: 10.1007/s11596-007-0201-3
|
|
Ozen S, Alikasifoglu M, Bakkaloglu A, Duzova A, Jarosova K, Nemcova D, Besbas N, Vencovsky J, Tuncbilek E. Tumour necrosis factor alphaG-->A-238 and G-->A-308 polymorphisms in juvenile idiopathic arthritis. Rheumatology (Oxford), 2002, 41(2): 223―227
doi: 10.1093/rheumatology/41.2.223
|
|
Beg A A, Baltimore D. Anessential role for NF-κ B in preventing TNF- induced cell death. Science, 1996, 274: 782―784
doi: 10.1126/science.274.5288.782
|
|
Hu Y H, Zeng K Q, Zhang M M, Tu S H, Lai X Y, Zhang W S. Effects of triptolide on the expression and activityof nuclear factor B of collagen-induced arthritis rats in synovium. Zhong Hua Feng Shi Bing Xue Za Zhi, 2004, 8(9): 515―518 (in Chinese)
|
|
Zhou Y L, Liu W, Zhang L. Investigation on effective dosage ofpolyglucoside of Tripterygium wilfordii in treating rheumatic arthritis. Tianjin Zhong Yi Yao, 2006, 23(1): 35―36 (in Chinese)
|
|
Li L X, Jin R M, Li Y K, Pang H F, Zhou Z L, Zhu Z L, Zhang J H. Safety marginand mechanism of anti-inflammaory effect of Tripterygium wil-fordii polyglycosidum. Zhong Guo Xin Yao Yu Lin Chuang Za Zhi, 2006, 25(2): 91―95 (in Chinese)
|
|
Bian X Q, Liu W, Liu X Y. Investigation about the relationshipof dosage of Tripterygium wilfordii and variety of X line. Tian Jin Yao Xue, 2006, 18(5): 34―35 (in Chinese)
|
|
Guo Y H, Tan K. Toxicity andsurvey of investigation on Tripterygium wilfordii. Zhong Yao Cai, 2007, 30(1): 112―117 (in Chinese)
|
|
Liu M M, Dong J, Yang Y J, Yang X L, Xu H B. Progress in research on triptolide. Zhong Guo Zhong Yao Za Zhi, 2005, 30(3): 170―174 (in Chinese)
|
|
Mattey D L, Gonzalez-Gay M A, Garcia-Porrua C, Thomson W, Hajeer A H, Ollier W E. Influence of HLA-DRB1 andTNF microsatellite polymorphisms on the expression of extraarticularmanifestations in rheumatoid arthritis patients from northwest Spain. Clin Exp Rheumatol, 2001, 19(6): 703―708
|
|
Modesto C, Patiño-García A, Sotillo-Piñeiro E, Merino J, García-Consuegra J, Merino R, Rua M J, Sierrasesúmaga L, Arnal C. TNF-alpha promoter gene polymorphismsin Spanish children with persistent oligoarticular and systemic-onsetjuvenile idiopathic arthritis. Scand JRheumatol, 2005, 34(6): 451―454
doi: 10.1080/03009740510026652
|
|
Törn C, Hillman M, Sanjeevi C B, Landin-Olsson M. Polymorphisms of TNF microsatellite marker and HLA-DR-DQin diabetes mellitus-a study in 609 Swedish subjects. Hum Immunol, 2006, 67(7): 527―534
doi: 10.1016/j.humimm.2006.04.002
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