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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med Chin    2010, Vol. 4 Issue (3) : 329-335     DOI: 10.1007/s11684-010-0091-9
RESEARCH ARTICLE |
Effects of resistin on skeletal glucose metabolism
Fang-Ping LI(), Zhi-Zhen LI, Miao ZHANG, Li YAN, Zu-Zhi FU
Department of Endocrinology, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China
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Abstract  

Resistin is an adipokine highly related to insulin resistance (IR). The purpose of our research was to investigate how resistin influences skeletal glucose metabolism and explore its mechanisms. We constructed the recombinant plasmid pcDNA3.1 expressing resistin and then transfected it into C2C12 myocytes. The expression of resistin in C2C12 myocytes was detected by Western blotting. Glucose uptake was measured by 3H labeled glucose; glucose oxidation and glycogen synthesis was detected with 14C-labeled glucose. GLUT4 mRNA was measured by reverse transcription polymerase chain reaction (RT-PCR). We observed that resistin was expressed in transfected myocytes, and resistin decreased insulin induced glucose uptake rate by 28%–31% and inhibited the expression of GLUT4 mRNA. However, there was no significant difference in basal glucose uptake, and glucose oxidation and glycogen synthesis remained unchanged in all groups. It is concluded that resistin inhibits insulin induced glucose uptake in myocytes by downregulating the expression of GLUT4 and it has no effects on glucose oxidation and glycogen synthesis. Our findings may provide a clue to understand the roles of resistin in the pathogenesis of skeletal IR.

Keywords resistin      insulin resistance      skeletal muscle     
Corresponding Authors: LI Fang-Ping,Email:amiy36@hotmail.com   
Issue Date: 05 September 2010
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-010-0091-9     OR     http://academic.hep.com.cn/fmd/EN/Y2010/V4/I3/329
Fig.1  Adipose tissue total RNA
Fig.2  RT-PCR product. 1: resistin cDNA; 2: marker.
Fig.3  The recombinant pGEM-T-resistin was verified by using R I and I digestion. Lane 1: pGEM-T-resistin was digested into pGEM-T vector (3000 bp) and inserted DNA (355 bp); lane 2: the recombinant pGEM-T-resistin; lane 3: marker.
Fig.4  Filamentous and myotubes ìnside the C2C12 myocytes (× 200)
Fig.5  Filamentous and myotubes inside the C2C12 myocytes (× 12000)
Fig.6  Result of Western blot assay. Lane 1: Marker; lanes 2 and 3: control group; lanes 4 and 5: transient group; lanes 6 and 7: vector group; lanes 8 and 9: stable group.
glucose uptake (pmol/106 cells·10 min)
groupsbaselineinsulin
control4.0±0.337.81±0.87
transient3.85±0.375.63±1.56
vector4.20±0.237.88±1.50
stable4.21±0.685.79±1.26
P value0.4540.011
Tab.1  Effect of resistin on glucose uptake in C2C12 myocytes
glucose oxidation (pmol/h·106 cells)
groupsbaselineinsulin
control0.89±0.194.25±1.71
transient1.16±0.725.19±1.35
vector1.18±0.814.72±0.82
stable0.74±0.434.52±2.20
P value0.5190.781
Tab.2  Effect of resistin on glucose oxidation in C2C12 myocytes
glycogen synthesis (pmol/h·106cells)
groupsbaselineinsulin
control 1.09±0.672.44±0.43
transient0.89±0.351.99±0.50
vector1.06±0.452.80±0.21
stable0.89±0.232.49±0.82
P value0.5820.218
Tab.3  Effect of resistin on glycogen synthesis in C2C12 myocytes
Fig.7  Resistin inhibited GLUT4 mRNA expression in C2C12 myocytes. 1: Marker; 2: control group; 3: transient group; 4: vector group; 5: stable group.
groupsGLUT4 mRNA
control0.73±0.11
transient0.53±0.16
vector0.69±0.08
stable0.49±0.09
P value0.041
Tab.4  Effect of resistin on GLUT4 mRNA in C2C12
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