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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med    2011, Vol. 5 Issue (1) : 101-105    https://doi.org/10.1007/s11684-011-0111-4
RESEARCH ARTICLE
Downregulation effects of beta-elemene on the levels of plasma endotoxin, serum TNF-alpha, and hepatic CD14 expression in rats with liver fibrosis
Jianguo LIU1, Zhe ZHANG2, Jiechang GAO1, Jiwen XIE1(), Lin YANG1, Shenjun HU1
1. Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China; 2. Department of Oncology, The Second Staff Hospital of Wuhan Iron and Steel (Group) Corp., Wuhan 430085, China
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Abstract

It has been demonstrated that β-elemene could protect against carbon tetrachloride (CCl4)-induced liver fibrosis in our laboratory work, and the aim of this paper is to reveal the protective mechanisms of β-elemene. The hepatic fibrosis experimental model was induced by the hypodermical injection of CCl4 in Wistar male rats. β-elemene was intraperitoneally administered into rats for 8 weeks (0.1 mL/100 g bodyweight per day), and plasma endotoxin content was assayed by biochemistry. The serum TNF-α level was detected using radioactive immunity. CD14 expression in rat livers was measured by immunohistochemistry and Western blot. The results showed that β-elemene can downregulate the levels of plasma endotoxins, serum TNF-α, and hepatic CD14 expression in rats with liver fibrosis. β-elemene plays an important role in downregulating the lipopolysaccharide signal transduction pathway, a significant pathway in hepatic fibrosis development.

Keywords liver fibrosis      beta-elemene      endotoxin      CD14     
Corresponding Author(s): XIE Jiwen,Email:xiejiwen@126.com   
Issue Date: 05 March 2011
 Cite this article:   
Jianguo LIU,Zhe ZHANG,Jiechang GAO, et al. Downregulation effects of beta-elemene on the levels of plasma endotoxin, serum TNF-alpha, and hepatic CD14 expression in rats with liver fibrosis[J]. Front Med, 2011, 5(1): 101-105.
 URL:  
https://academic.hep.com.cn/fmd/EN/10.1007/s11684-011-0111-4
https://academic.hep.com.cn/fmd/EN/Y2011/V5/I1/101
Fig.1  CD14 immunohistochemical staining of liver sections from rats treated with β-elemene (DAB × 20). CCl group; β-elemene group; Control group.
Fig.2  Western blotting of CD14 protein.
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