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Downregulation effects of beta-elemene on the levels of plasma endotoxin, serum TNF-alpha, and hepatic CD14 expression in rats with liver fibrosis |
Jianguo LIU1, Zhe ZHANG2, Jiechang GAO1, Jiwen XIE1(), Lin YANG1, Shenjun HU1 |
1. Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China; 2. Department of Oncology, The Second Staff Hospital of Wuhan Iron and Steel (Group) Corp., Wuhan 430085, China |
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Abstract It has been demonstrated that β-elemene could protect against carbon tetrachloride (CCl4)-induced liver fibrosis in our laboratory work, and the aim of this paper is to reveal the protective mechanisms of β-elemene. The hepatic fibrosis experimental model was induced by the hypodermical injection of CCl4 in Wistar male rats. β-elemene was intraperitoneally administered into rats for 8 weeks (0.1 mL/100 g bodyweight per day), and plasma endotoxin content was assayed by biochemistry. The serum TNF-α level was detected using radioactive immunity. CD14 expression in rat livers was measured by immunohistochemistry and Western blot. The results showed that β-elemene can downregulate the levels of plasma endotoxins, serum TNF-α, and hepatic CD14 expression in rats with liver fibrosis. β-elemene plays an important role in downregulating the lipopolysaccharide signal transduction pathway, a significant pathway in hepatic fibrosis development.
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Keywords
liver fibrosis
beta-elemene
endotoxin
CD14
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Corresponding Author(s):
XIE Jiwen,Email:xiejiwen@126.com
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Issue Date: 05 March 2011
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1 |
Moreira R K. Hepatic stellate cells and liver fibrosis. Arch Pathol Lab Med , 2007, 131(11): 1728–1734 pmid:17979495
|
2 |
Tangkijvanich P, Yee H F Jr. Cirrhosis—can we reverse hepatic fibrosis? Eur J Surg Suppl , 2002, (587): 100–112 pmid:16144208
|
3 |
Hu S J, Yang L, Zhu Q J, Peng H G. Effect of β-elemene on the expression of transforming growth factor-β1, a-smooth muscle actin and collagen-I in rats with hepatic fibrosis. Shijie Huaren Xiaohua Zazhi , 2007, 15(12): 1324–1330
|
4 |
Rui Z H U, Ling Y A N G, Lin S H E N, Jin Y E, Jianguo L I U, Shenjun H U. ANGII-AT1 Receptor Pathway Is Involved in the Anti-fibrotic Effect of β-elemene. J Huazhong Univ Sci Technol , 2009, 29(2): 177–181 (Med Sci) doi: 10.1007/s11596-009-0208-z
|
5 |
Isayama F, Hines I N, Kremer M, Milton R J, Byrd C L, Perry A W, McKim S E, Parsons C, Rippe R A, Wheeler M D. LPS signaling enhances hepatic fibrogenesis caused by experimental cholestasis in mice. Am J Physiol Gastrointest Liver Physiol , 2006, 290(6): G1318–G1328 doi: 10.1152/ajpgi.00405.2005 pmid:16439470
|
6 |
Jamall I S, Finelli V N, Que Hee S S. A simple method to determine nanogram levels of 4-hydroxyproline in biological tissues. Anal Biochem , 1981, 112(1): 70–75 doi: 10.1016/0003-2697(81)90261-X pmid:7258630
|
7 |
Su G L. Lipopolysaccharides in liver injury: molecular mechanisms of Kupffer cell activation. Am J Physiol Gastrointest Liver Physiol , 2002, 283(2): G256–G265
|
8 |
Mathison J C, Ulevitch R J. The clearance, tissue distribution, and cellular localization of intravenously injected lipopolysaccharide in rabbits. J Immunol , 1979, 123(5): 2133–2143 pmid:489976
|
9 |
Zlydaszyk J C, Moon R J. Fate of 51Cr-labeled lipopolysaccharide in tissue culture cells and livers of normal mice. Infect Immun , 1976, 14(1): 100–105 pmid:780270
|
10 |
Bigatello L M, Broitman S A, Fattori L, Di Paoli M, Pontello M, Bevilacqua G, Nespoli A. Endotoxemia, encephalopathy, and mortality in cirrhotic patients. Am J Gastroenterol , 1987, 82(1): 11–15 pmid:3799574
|
11 |
Lin R S, Lee F Y, Lee S D, Tsai Y T, Lin H C, Lu R H, Hsu W C, Huang C C, Wang S S, Lo K J. Endotoxemia in patients with chronic liver diseases: relationship to severity of liver diseases, presence of esophageal varices, and hyperdynamic circulation. J Hepatol , 1995, 22(2): 165–172 doi: 10.1016/0168-8278(95)80424-2 pmid:7790704
|
12 |
Nolan J P. The role of endotoxin in liver injury. Gastroenterology , 1975, 69(6): 1346–1356 pmid:1104401
|
13 |
Nolan J P, Camara D S. Intestinal endotoxins as co-factors in liver injury. Immunol Invest , 1989, 18(1-4): 325–337 doi: 10.3109/08820138909112246 pmid:2659515
|
14 |
Adachi Y, Moore L E, Bradford B U, Gao W, Thurman R G. Antibiotics prevent liver injury in rats following long-term exposure to ethanol. Gastroenterology , 1995, 108(1): 218–224 doi: 10.1016/0016-5085(95)90027-6 pmid:7806045
|
15 |
Bhagwandeen B S, Apte M, Manwarring L, Dickeson J. Endotoxin induced hepatic necrosis in rats on an alcohol diet. J Pathol , 1987, 152(1): 47–53 doi: 10.1002/path.1711520107 pmid:3305847
|
16 |
Czaja M J, Xu J, Ju Y, Alt E, Schmiedeberg P. Lipopolysaccharide-neutralizing antibody reduces hepatocyte injury from acute hepatotoxin administration. Hepatology , 1994, 19(5): 1282–1289 doi: 10.1002/hep.1840190529 pmid:8175153
|
17 |
Moulin F, Copple B L, Ganey P E, Roth R A. Hepatic and extrahepatic factors critical for liver injury during lipopolysaccharide exposure. Am J Physiol Gastrointest Liver Physiol , 2001, 281(6): G1423–G1431 pmid:11705747
|
18 |
Kamimura S, Tsukamoto H. Cytokine gene expression by Kupffer cells in experimental alcoholic liver disease. Hepatology , 1995, 22(4 Pt 1): 1304–1309 pmid:7557885
|
19 |
McClain C J, Barve S, Deaciuc I, Kugelmas M, Hill D. Cytokines in alcoholic liver disease. Semin Liver Dis , 1999, 19(2): 205–219 doi: 10.1055/s-2007-1007110 pmid:10422201
|
21 |
Matsuura K, Ishida T, Setoguchi M, Higuchi Y, Akizuki S, Yamamoto S. Upregulation of mouse CD14 expression in Kupffer cells by lipopolysaccharide. J Exp Med , 1994, 179(5): 1671–1676 doi: 10.1084/jem.179.5.1671 pmid:7513013
|
22 |
Fearns C, Kravchenko V V, Ulevitch R J, Loskutoff D J. Murine CD14 gene expression in vivo: extramyeloid synthesis and regulation by lipopolysaccharide. J Exp Med , 1995, 181(3): 857–866 doi: 10.1084/jem.181.3.857 pmid:7532683
|
23 |
Enomoto N, Ikejima K, Yamashina S, Hirose M, Shimizu H, Kitamura T, Takei Y, Sato And N, Thurman R G. Kupffer cell sensitization by alcohol involves increased permeability to gut-derived endotoxin. Alcohol Clin Exp Res , 2001, 25(6 Suppl): 51S–54S pmid:11410742
|
24 |
Tracy T F Jr, Fox E S. CD14-lipopolysaccharide receptor activity in hepatic macrophages after cholestatic liver injury. Surgery , 1995, 118(2): 371–377 doi: 10.1016/S0039-6060(05)80347-2 pmid:7543706
|
25 |
Su G L, Rahemtulla A, Thomas P, Klein R D, Wang S C, Nanji A A. CD14 and lipopolysaccharide binding protein expression in a rat model of alcoholic liver disease. Am J Pathol , 1998, 152(3): 841–849 pmid:9502426
|
26 |
Ferrero E, Jiao D, Tsuberi B Z, Tesio L, Rong G W, Haziot A, Goyert S M. Transgenic mice expressing human CD14 are hypersensitive to lipopolysaccharide. Proc Natl Acad Sci USA , 1993, 90(6): 2380–2384 doi: 10.1073/pnas.90.6.2380 pmid:7681594
|
27 |
Haziot A, Ferrero E, Lin X Y, Stewart C L, Goyert S M. CD14-deficient mice are exquisitely insensitive to the effects of LPS. Prog Clin Biol Res , 1995, 392: 349–351 pmid:8524940
|
29 |
Tomita M, Yamamoto K, Kobashi H, Ohmoto M, Tsuji T. Immunohistochemical phenotyping of liver macrophages in normal and diseased human liver. Hepatology , 1994, 20(2): 317-325 doi: 10.1002/hep.1840200208 pmid:7519162
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