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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med    2011, Vol. 5 Issue (1) : 106-110     DOI: 10.1007/s11684-011-0113-2
RESEARCH ARTICLE |
A better way to do small-for-size liver transplantation in rats
Jiang LI1, Yu HOU2, Jing LIU3, Bin LIU1(), Li LI3
1. Department of Hepatobiliary Surgery, First Affiliated Hospital of Kunming Medical College, Kunming 650032, China; 2. Department of Radiation Oncology, Third Affiliated Hospital of Kunming Medical College, Kunming 650181, China; 3. First Department of Hepatobiliary Surgery, Ganmeit Affiliated Hospital of Kunming Medical College, Kunming 650031, China
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Abstract  

Establishing a model for small-for-size liver transplantation is the basis for this study of partial and living donor graft liver transplantation. This study aims to explore a simpler and more effective way of establishing a 30% small-for-size liver transplantation in rats. Sprague-Dawley rats were selected as the donors and recipients. Small-for-size orthotopic liver transplantation was performed using Kamada’s two-cuff method. The donor’s liver was flushed via the abdominal aorta and hepatectomy was performed in situ. The animals were divided into three groups depending on the graft selected, with 40 pairs of rats in each group. In group I, the median lobe of the liver was used as graft; in group II, the right half of the median lobe and the right lobe were used as graft; and in group III, the median and right lobes were used as graft. In groups I and II, the bodyweights of donors were the same as those of recipients; however, in group III the bodyweights of donors were 100–120 g less than those of the recipients. The duration needed for transplantation, the 7-day survival rates, and the technical complication rates were compared among these three groups. The time required for hepatectomy was shorter in group III compared with groups I and II (8.8?±?0.7 min vs. 11.5?±?1.1 min and 10.1?±?1.0 min, P = 0.001). The cold ischemia time for the grafts, the anhepatic times, and the transplantation times for the recipients were not significantly different among the three groups. Compared with groups I and II, the incidence of bleeding, bile leakage, and inferior vena caval strictures were significantly decreased in group III (P<0.05). No significant differences between the three groups were found based on other complications after the operation (P>0.05). Group III had better 7-day survival rates and longer median survival times but the differences were not statistically significant. The method of small for donor bodyweight using the median and right lobes for grafting may be a more effective and simpler way of establishing a 30% small-for-size liver transplantation in rats, as shown by the shorter hepatectomy time and the occurrence of fewer complications after the operation.

Keywords liver transplantation      small-for-size      rats     
Corresponding Authors: LIU Bin,Email:liubin597088@yahoo.com.cn   
Issue Date: 05 March 2011
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-011-0113-2     OR     http://academic.hep.com.cn/fmd/EN/Y2011/V5/I1/106
GroupIGroupIIGroupIII
Donor bodyweight (g)281.7±17.3267.8±14.3234.4±18.1
Recipient bodyweight (g)290.0±22.1283.5±19.4347.6±21.7
Graft weight (g)3.03±0.362.66±0.243.27±0.33
Recipient liver weight (g)9.96±1.968.79±0.8012.57±1.27
Graft weight ratio (%)30.92±3.6130.93±2.0925.93±2.22
Graft-to-bodyweight ratio (%)1.04±0.090.94±0.060.93±0.06
Tab.1  Body weight and graft size of donors and recipients.
GroupALT (IU/L)TB (μmol/L)AMON (μmol/L)
Day 1Day 7Day 1Day 7Day 1Day 7
I354.2±29.1180.4±13.622.0±5.916.2±5.9318.4±15.3150.0±30.7
II346.0±29.4174.0±24.323.2±6.617.8±5.8323.8±49.4152.8±33.2
III314.6±33.7159.0±14.617.8±3.312.8±4.1275.8±50.0114.2±26.2
Tab.2  Laboratory results post-operation.
Fig.1  Histology of the grafts on day 7 after the operation. I-III liver sections are from groups I-III, respectively. Small amounts of lymphocytic infiltrates around the portal area (→) and mild acute rejection could be seen under light microscopy with hematoxylin and eosin staining. (Original magnification 200 × )
Fig.2  Kaplan–Meier survival curve. The survival rates and median survival times of the rats in group III were the highest among the three groups but the differences were not statistically significant.
Fig.3  Liver transplantation. 1; caudal lobe: 2; right lobe: 3a; right half of median lobe: 3b; left half of median lobe: 4; left lobe: 5, disciform lobe (they are double). ( Liver lobes of the rat. ( The graft of group III. Suprahepatic vein cava anastomoses (→). ( Cuff technique and graft reperfusion. IVC :inferior vena cava.
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