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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med    2011, Vol. 5 Issue (1) : 61-69     DOI: 10.1007/s11684-011-0122-1
REVIEW |
Tissue engineering of cartilage, tendon and bone
Hengyun SUN1,2, Wei LIU1,2, Guangdong ZHOU1,2, Wenjie ZHANG1,2, Lei CUI1,2, Yilin CAO1,2()
1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai 200011, China; 2. National Tissue Engineering Center of China, Shanghai 200241, China
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Abstract  

Tissue engineering aims to produce a functional tissue replacement to repair defects. Tissue reconstruction is an essential step toward the clinical application of engineered tissues. Significant progress has recently been achieved in this field. In our laboratory, we focus on construction of cartilage, tendon and bone. The purpose of this review was to summarize the advances in the engineering of these three tissues, particularly focusing on tissue regeneration and defect repair in our laboratory. In cartilage engineering, articular cartilage was reconstructed and defects were repaired in animal models. More sophisticated tissues, such as cartilage in the ear and trachea, were reconstructed both in vitro and in vivo with specific shapes and sizes. Engineered tendon was generated in vitro and in vivo in many animal models with tenocytes or dermal fibroblasts in combination with appropriate mechanical loading. Cranial and limb bone defects were also successfully regenerated and repaired in large animals. Based on sophisticated animal studies, several clinical trials of engineered bone have been launched with promising preliminary results, displaying the high potential for clinical application.

Keywords Tissue engineering      cartilage      bone      tendon      recent advances     
Corresponding Authors: CAO Yilin,Email:yilincao@yahoo.com   
Issue Date: 05 March 2011
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-011-0122-1     OR     http://academic.hep.com.cn/fmd/EN/Y2011/V5/I1/61
Fig.1  Comparison of chondrocyte- and BMSC-mediated repair of osteochondral defects at six months. Gross view (above) and cross-sectional view (below) of repaired articular cartilage defects were shown (arrowheads indicate the repaired defects). (Reprinted by permission of )
Fig.2  Preparation and shape analysis of the ear-shaped scaffolds and constructs. 3D image of the normal ear; the mirror image of ; The half-sized resin positive mold; outer part of the negative mold (left) and inner part of the negative mold (right); the ear-shaped PLA/PGA scaffold; laser scan image of ; color map of ; laser scan image of ; color map of , compared to ; ear-shaped cell-scaffold constructs at 12 weeks . color map of , compared to . (Reprinted by permission of )
Fig.3  Gross view , H&E staining , polarized microscopic view and transmission electron microscopic view of engineered tendons with mechanical loading for 14 weeks. (Reprinted by permission of )
Fig.4  Gross view of repaired mandibles with BMSCs/coral grafts and contralateral bones at 32 weeks postoperation. (Reprinted by permission of )
Fig.5  X-ray evaluation of tissue-engineered bone repair of a femoral bone defect at 8 months post-operation. Control group; Experimental group. (Reprinted by permission of )
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