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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med    2011, Vol. 5 Issue (2) : 195-202     DOI: 10.1007/s11684-011-0134-x
Overview of the quality standard research of traditional Chinese medicine
Huimin Gao1,2, Zhimin Wang1,2(email.png), Yujuan Li3, Zhongzhi Qian4
1. 1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 2. 2. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China; 3. 3. Beijing Institute of Technology, Beijing, 100081, China; 4. 4. Chinese Pharmacopoeia Commission, Beijing 100061, China
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Traditional Chinese medicine (TCM) has been widely used for the prevention and treatment of various diseases for a long time in China. Due to its proven efficacy, wide applications, and low side effect, TCM has increasingly attracted worldwide attention. However, one of the biggest challenges facing the clinical practice of TCM is the uncontrollable quality. In this review, the progress of the development and the current status of quality standard as well as new quality control techniques introduced in Chinese Pharmacopoeia (2010 edition), such as liquid chromatography hyphenated mass spectrometry (LC-MS), fingerprint, quantitative analysis of multi-components by single-marker (QAMS), thin layer chromatography bio-autographic assay (TLC-BAA), and DNA molecular marker technique, are briefly overviewed.

Keywords traditional Chinese medicine      quality standard      quality control      quantitative analysis of multi-components by single-marker     
Corresponding Authors: Wang Zhimin,   
Issue Date: 05 June 2011
URL:     OR
Fig.1  Bio-fingerprint profiles of and its bio-samples-everted intestinal sac model, a–f. Bio-samples-everted intestinal sac model collected at 15, 30, 45, 60, 90, 120 min, g. Extract of , 1) gallic acid, 2) paeoniflorin sulfonate, 3) albiflorin, and 4) paeoniflorin. The chromatographic profiles were obtained on analytical C column, with gradient elution at 230 nm and the mobile phase consisting of acetonitrile (A) and water (adjusted the pH to 2.7 with phosphoric acid, B). The elution procedure is as follows: 0-20 min, 5%-15% A, 20-40 min, 15%-20% A, 40-50 min, 20%-23% A, 50-70 min, and 23%-40% A.
Fig.2  Time/min
Reference fingerprint profile of described in the ChP (2010 edition) [], 2) protocatechualdehyde, 5) rosmarinic acid, 6) shikonin, and 7) salvianolic acid B
AlkaloidsRelative Rf values
Tab.1  Relative R values of four alkaloids in []
Fig.3  Time/min
Typical chromatographic profile of . 1) jatrorrhizine, 2) epi-berberine, 3) coptisine, 4) palmatine, and 5) berberine. The chromatographic profile was obtained at 345 nm on a analytical C column. The mobile phase contained acetonitrile (A) and water (50 mmol·L potassium dihydrogen phosphate and 25 mmol·L sodium dodecyl sulfonate were added; adjusted the pH to 3.0).
Fig.4  TLC-bioautographic chromatogram of and screening of the antioxidant components as well as their online identification in
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