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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med    2011, Vol. 5 Issue (3) : 294-301     DOI: 10.1007/s11684-011-0144-8
RESEARCH ARTICLE |
c-Fos expression in rat brainstem following intake of sucrose or saccharin
Ke Chen1, Jianqun Yan1,2(), Jinrong Li1, Bo Lv1, Xiaolin Zhao1
1. Department of Physiology and Pathophysiology, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China; 2. Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China
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Abstract  

To examine whether the activation of brainstem neurons during intake of a sweet tastant is due to orosensory signals or post-ingestive factors, we compared the distribution of c-Fos-like immunoreactivity (c-FLI) in the nucleus of the solitary tract (NST) and parabrachial nucleus (PBN) of brainstem following ingestion of 0.25 M sucrose or 0.005 M saccharin solutions. Immunopositive neurons were localized mainly in the middle zone of the PBN and four rostral-caudal subregions of the NST. Intake of sucrose increased the number of FLI neurons in almost every subnucleus of the PBN (F(2,13) = 7.610, P = 0.023), in addition to the caudal NST at the level of the area postrema (F(2,13) = 10.777, P = 0.003) and the NST intermediate zone (F(2,13) = 7.193, P = 0.014). No significant increase in the number of c-Fos positive neurons was detected in response to saccharin ingestion, although there was a trend towards a modest increase in a few select NST and PBN nuclei. These results suggest that the PBN and NST may be involved in sweet taste perception and modulation of sweet tastant intake, but the significantly enhanced intensity of Fos expression induced by sucrose indicates that PBN/NST neuronal activity is driven by the integrated effects of sweet taste sensation and post-ingestive signals.

Keywords c-Fos      parabrachial      the nucleus of the solitary tract      sweet tastant      rat     
Corresponding Authors: Yan Jianqun,Email:jqyan810@gmail.com   
Issue Date: 05 September 2011
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-011-0144-8     OR     http://academic.hep.com.cn/fmd/EN/Y2011/V5/I3/294
Fig.1  The changes in weight, weight gain, food intake, and water intake after water restriction (on day 2). A. body weight; B. body weight gain; C. food intake; D. water intake. Values are presented as means±SEM. a, b and c indicate significant difference from baseline after water restriction in the control group (a), sucrose group (b), and saccharin group (c) ( = 5 per treatment group).
Fig.2  The number of FLI-positive neurons in the NST induced by sweetener intake. After sucrose solution intake, the number of FLI-positive neurons in the N2 and N3 regions increased significantly. Values are presented as means±SEM. **<0.01, the difference between the sucrose group and control group; <0.05, the difference between the saccharin group and the sucrose group. There were 5 rats in each treatment group.
Fig.3  The distribution of FLI-positive neurons in the NST-N3 region of rats induced by intake of sweet taste solutions. Sucrose solution elicited robust FLI in this area. A, B, and C indicate control group, sucrose group, and saccharin group, respectively. D is the schematic neuroanatomic diagram of the N3 region. NST, the nucleus of the solitary tract; sol, solitary tract; 4V, fourth ventricle ( = 5). Scale bar= 100 μm.
Fig.4  The number of FLI-positive neurons in subnuclei of the PBN induced by sweet tastant intake. Sucrose elicited marked c-FLI in the vl, dl, cl, and medial nucleus of the PBN. Values are presented as means±SEM of five rats per treatment group. *<0.05, **<0.01, compared with control rats; <0.05, <0.01, compared with sucrose group.
Fig.5  The distribution of FLI-positive neurons in the parabrachial nucleus induced by sucrose or saccharin intake. Sucrose solution induced vigorous c-FLI in this area, while intake of saccharin solution elicited negligible expression compared to the control group. (A–C). Micrographs are stained slices from a control rat (A), sucrose-fed rat (B), and saccharin-fed rat (C). SCP, superior cerebellar peduncle (brachium conjunctivum). Scale bar= 100 μm.
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