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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front Med    2012, Vol. 6 Issue (4) : 411-415     DOI: 10.1007/s11684-012-0226-2
Tramadol reinforces antidepressant effects of ketamine with increased levels of brain-derived neurotrophic factor and tropomyosin-related kinase B in rat hippocampus
Chun Yang, Xiaomin Li, Nan Wang, Shixia Xu, Jianjun Yang, Zhiqiang Zhou()
Department of Anesthesiology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, China
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Ketamine exerts rapid and robust antidepressant properties in both animal models and depressed patients and tramadol possesses potential antidepressant effects. Brain-derived neurotrophic factor (BDNF) is an important biomarker for mood disorders and tropomyosin-related kinase B (TrkB) is a high affinity catalytic receptor for BDNF. We hypothesized that tramadol pretreatment might reinforce ketamine-elicited antidepressant effects with significant changes in hippocampal BDNF and TrkB levels in rats. Immobility time of rats receiving different treatment in the forced swimming test (FST) was observed. Levels of BDNF and TrkB in hippocampus were measured by enzyme linked immunosorbent assay. Results showed that tramadol (5 mg/kg) administrated alone neither elicited antidepressant effects nor altered BDNF or TrkB level. However, pretreatment with tramadol (5 mg/kg) enhanced the ketamine (10 mg/kg) -elicited antidepressant effects and upregulated the BDNF and TrkB levels in hippocampus. In conclusion, tramadol pretreatment reinforces the ketamine-elicited antidepressant effects, which is associated with the increased levels of BDNF and TrkB in rat hippocampus.

Keywords tramadol      ketamine      antidepressant      brain-derived neurotrophic factor      tropomyosin-related kinase B     
Corresponding Authors: Zhou Zhiqiang,   
Issue Date: 05 December 2012
URL:     OR
Fig.1  Effects of different interventions (saline+ saline, tramadol 5 mg/kg+ saline, saline+ ketamine 10 mg/kg, and tramadol 5 mg/kg+ ketamine 10 mg/kg, i.p.) on the immobility time of rats in the FST. Bars represent means±SD. *<0.05, the other three groups vs. the saline+ saline group; <0.05, the tramadol+ ketamine group vs. the saline+ ketamine group.
Fig.2  Effects of different interventions (saline+ saline, tramadol 5 mg/kg+ saline, saline+ ketamine 10 mg/kg, and tramadol 5 mg/kg+ ketamine 10 mg/kg, i.p.) on the expression of hippocampal BDNF of rats in the FST. Bars represent means±SD. *<0.05, the other three groups vs. the saline+ saline group; <0.05, the tramadol+ ketamine group vs. the saline+ ketamine group.
Fig.3  Effects of different interventions (saline+ saline, tramadol 5 mg/kg+ saline, saline+ ketamine 10 mg/kg, and tramadol 5 mg/kg+ ketamine 10 mg/kg, i.p.) on the expression of hippocampal TrkB of rats in the FST. Bars represent means±SD. *<0.05, the other three groups vs. the saline+ saline group; <0.05, the tramadol+ ketamine group vs. the saline+ ketamine group.
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