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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2015, Vol. 9 Issue (3) : 368-373     DOI: 10.1007/s11684-015-0403-1
RESEARCH ARTICLE |
Glucagon-like peptide-2 exhibits protective effect on hepatic ischemia-reperfusion injury in rats
Naci Topaloğlu1,Adem Küçük2,Şule Yıldırım1,*(),Mustafa Tekin1,Havva Erdem3,Mustafa Deniz4
1. ?anakkale Onsekiz Mart University Medical Faculty, Department of Pediatrics, ?anakkale, Turkey
2. Düzce Atatürk State Hospital, Department of Pediatric Surgery, Düzce, Turkey
3. Düzce University Medical Faculty, Department of Pathology, Düzce, Turkey
4. ?anakkale Onsekiz Mart University Medical Faculty, Department of Physiology, ?anakkale, Turkey
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Abstract  

Glucagon-like peptide-2 (GLP-2) has potent anti-inflammatory effects and protects against experimental ischemia/reperfusion (I/R) injury in pulmonary, intestinal, and myocardial tissue. However, its protective abilities against I/R injury in the liver are unknown. We investigated the potential role of GLP-2 pretreatment on hepatic I/R injury in rats. A total of 24 rats were randomly divided into three groups (n = 8). The first group was the control group; the second group was the vehicle-treated hepatic ischemia/reperfusion (HIR, vehicle saline-treated) group; and the third group was the GLP-2 pretreated I/R (GLP2-IR) group. Each rat in the third group was intraperitoneally administered 5 μg GLP-2 for 5 d before the procedure. A portal triad was created to induce ischemia with a vascular atraumatic clamp. After 40 min, the clamp was released to initiate hepatic reperfusion for 6 h. Blood samples and tissue specimens from the liver were obtained. Alanine aminotransferase, aspartate aminotransferase, and total bilirubin levels significantly increased in the saline-treated HIR group (P<0.001), whereas GLP-2 pretreatment significantly decreased their levels (P<0.01). Our data suggested that GLP-2 pretreatment may have a protective effect on liver I/R injury. However, dose-response studies are necessary to determine the most effective dose.

Keywords ischemia/reperfusion      liver      glucagon-like peptide-2      alanine aminotransferase     
Corresponding Authors: ?ule Y?ld?r?m   
Just Accepted Date: 07 July 2015   Online First Date: 21 August 2015    Issue Date: 26 August 2015
URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-015-0403-1     OR     http://academic.hep.com.cn/fmd/EN/Y2015/V9/I3/368
Control (mean±SEM) HIR (mean±SEM) GLP2-IR (mean±SEM)
AST (n = 8) (U/L) 102.50±10.29 435.71±26.00 223.87±58.00
ALT (n = 8) (U/L) 34.75±3.57 365.71±55.00 164.14±65.00
Total bilirubin (n = 8) (mg/dl) 0.06±0.08 0.12±0.01 0.05±0.00
Total protein (n = 8) (g/dl) 5.08±0.13 5.37±0.09 5.37±0.17
GGT (n = 8) (U/L) 0.12±0.12 1.37±0.32 0.62±0.183
ALP (n = 8) (U/L) 74.11±13.00 134.62±9.56 128.87±7.13
Tab.1  Serum levels of AST, ALT, total bilirubin, total protein, GGT and ALP of rats in the groups
Fig.1  Histopathological liver specimens from rats. (A) Control group: normal liver tissue. (B) Specimens of HIR group. Liver specimens after ischemia reperfusion exhibited focal necrosis and infiltration of leukocytes. (C) Specimens of GLP2-IR group. GLP-2 treatment did not significantly decrease these pathological changes (P?>?0.05).
Fig.2  The effect of saline, I/R, and GLP-2 pretreatment on microscopic damage scores. *P?<?0.05; compared with the control group.
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