Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium

doi:10.1007/s11684-016-0459-6

Front. Med.

2016, Vol. 10

Issue (3) : 330-335 https://doi.org/10.1007/s11684-016-0459-6

RESEARCH ARTICLE

Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium

Yan Chen^1,^2,³,Wenyan Li^1,³,Wen Li^1,^2,³,Renjie Chai^4,^5,^*(

),Huawei Li^1,^3,^6,^*(

)

¹. Otorhinolaryngology Department of Affiliated Eye and ENT Hospital, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200031, China
². Central Laboratory, Affiliated Eye and ENT Hospital, Fudan University, Shanghai 200031, China
³. Key Laboratory of Hearing Medicine of the National Health and Family Planning Commission, Shanghai 200031, China
⁴. MOE Key Laboratory of Developmental Genes and Human Disease, State Key Laboratory of Bioelectronics, Institute of Life Sciences, Southeast University, Nanjing 210096, China
⁵. Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China
⁶. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China

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Abstract

The enhancer of zeste 2 polycomb repressive complex 2 subunit (Ezh2) is a histone-lysine N-methyltransferase enzyme that participates in DNA methylation. Ezh2 has also been reported to play crucial roles in stem cell proliferation and differentiation. However, the detailed expression profile of Ezh2 during mouse cochlear development has not been investigated. Here, we examined the spatiotemporal expression of Ezh2 in the cochlea during embryonic and postnatal development. Ezh2 expression began to be observed in the whole otocyst nuclei at embryonic day 9.5 (E9.5). At E12.5, Ezh2 was expressed in the nuclei of the cochlear prosensory epithelium. At E13.5 and E15.5, Ezh2 was expressed from the apical to the basal turns in the nuclei of the differentiating cochlear epithelium. At postnatal day (P) 0 and 7, the Ezh2 expression was located in the nuclei of the cochlear epithelium in all three turns and could be clearly seen in outer and inner hair cells, supporting cells, the stria vascularis, and spiral ganglion cells. Ezh2 continued to be expressed in the cochlear epithelium of adult mice. Our results provide the basic Ezh2 expression pattern and might be useful for further investigating the detailed role of Ezh2 during cochlear development.

Keywords polycomb repressive complex Ezh2 expression inner ear cochlea development

Corresponding Author(s): Renjie Chai,Huawei Li

Just Accepted Date: 07 July 2016 Online First Date: 27 July 2016 Issue Date: 30 August 2016

Cite this article:

Yan Chen,Wenyan Li,Wen Li, et al. Spatiotemporal expression of Ezh2 in the developing mouse cochlear sensory epithelium[J]. Front. Med., 2016, 10(3): 330-335.

URL:

https://academic.hep.com.cn/fmd/EN/10.1007/s11684-016-0459-6
https://academic.hep.com.cn/fmd/EN/Y2016/V10/I3/330

Fig.1 Ezh2 was expressed in the otocyst at E9.5 and E10.5. Triple immunofluorescence staining showed that Ezh2 staining was located in the nuclei of the whole otocyst.

Fig.2 Ezh2 was expressed in the developing cochlear duct. Representative images of E12.5 (A), E13.5 (B), and E15.5 (C) cochlear epithelium are shown.

Fig.3 Ezh2 was expressed in the sensory epithelium and the spiral ganglion at P0. (A) The image shows obvious Ezh2 staining in the cochlear sensory epithelium, the stria vascularis, and the spiral ganglion at low magnification. The arrows indicate the spiral ganglions. (B) The images show that Ezh2 was expressed in the Kölliker organ, inner and outer hair cells, and supporting cells. OHCs, outer hair cells; IHC, inner hair cell.

Fig.4 Ezh2 was expressed in the cochlear sensory epithelium at P7. Myosin 7a and Ezh2 staining (A) showed that Ezh2 was expressed in the inner and outer hair cells. Sox2 and Ezh2 staining (B) showed that Ezh2 was expressed in all supporting cells.

Fig.5 Ezh2 was expressed in the cochlear sensory epithelium at P35. Parvalbumin and Ezh2 staining (A) showed that Ezh2 was expressed in the inner and outer hair cells. Sox2 and Ezh2 staining (B) showed that Ezh2 was expressed in all supporting cells. OHCs, outer hair cells; IHC, inner hair cell. Negative control images with primary antibody omission are also shown (C).

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