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Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2017, Vol. 11 Issue (4) : 548-553    https://doi.org/10.1007/s11684-017-0597-5
RESEARCH ARTICLE |
Impact of HBV replication in peripheral blood mononuclear cell on HBV intrauterine transmission
Xiaohong Shi1, Xuefei Wang1, Xixi Xu1, Yongliang Feng1, Shuzhen Li1, Shuying Feng2, Bo Wang2, Suping Wang1()
1. Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China
2. Department of Obstetrics and Gynaecology, The Third People Hospital of Taiyuan City, Taiyuan 030001, China
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Abstract

This study determined the effect of hepatitis B virus (HBV) replication in peripheral blood mononuclear cell (PBMC) from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg-positive mothers and their neonates were recruited in this study. Within 24 h after birth, HBV serological markers, serum HBV DNA, PBMC HBV relaxed circular DNA (rcDNA), and covalently closed circular DNA (cccDNA) were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis. The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chi-square test and logistic regression. The rate of HBV intrauterine transmission was 8.00% (12/150) in the 150 neonates born to HBsAg-positive mothers. The positivities of PBMC HBV rcDNA and cccDNA in the HBsAg-positive mothers were 36.67% (55/150) and 10% (15/150), respectively. Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission (OR= 6.003, 95% CI: 1.249–28.855). Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA (OR= 3.896, 95% CI: 1.929–7.876) and PBMC HBV cccDNA (OR= 3.74, 95% CI: 1.186–11.793) in the HBsAg-positive mothers. Administration of hepatitis B immune globulin was a protective factor of PBMC HBV cccDNA (OR= 0.312, 95% CI: 0.102–0.954) during pregnancy. The positivity of PBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers (c2=5.087, P= 0.024). This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.

Keywords PBMC      HBV cccDNA      HBV rcDNA      HBV intrauterine transmission     
Corresponding Authors: Suping Wang   
Just Accepted Date: 30 October 2017   Online First Date: 22 November 2017    Issue Date: 04 December 2017
 Cite this article:   
Xiaohong Shi,Xuefei Wang,Xixi Xu, et al. Impact of HBV replication in peripheral blood mononuclear cell on HBV intrauterine transmission[J]. Front. Med., 2017, 11(4): 548-553.
 URL:  
http://academic.hep.com.cn/fmd/EN/10.1007/s11684-017-0597-5
http://academic.hep.com.cn/fmd/EN/Y2017/V11/I4/548
Characteristics HBV intrauterine transmission P
Yes ( n) No ( n)
Age of mother (year) M (QR) 27 (5) 27 (7) 0.961 a
Gestational weeks M (QR) 39.5 (1.5) 40 (1) 0.528 a
Neonatal weight (g) M (QR) 3400 (600) 3000 (250) 0.009* ,a
Gender of neonate Male 4 68 0.368 b
Female 8 66
Mode of delivery Vaginal delivery 8 84 0.767
Caesarean section 4 54
Educational level of mother <High school 9 79 0.361
≥High School 3 59
History of hepatitis Negative 9 113 0.698
Positive 3 25
Family history of HBV infection Negative 8 98 0.748
Positive 4 40
Hepatitis B vaccine injection Negative 12 125 0.601
Positive 0 13
HBIG administration Negative 4 48 1.000
Positive 8 90
History of blood transfusion Negative 12 134 1.000
Positive 0 4
History of acupuncture Negative 12 128 1.000
Positive 0 10
History of dental treatment Negative 11 101 0.297
Positive 1 37
History of abortion or induced labor Negative 7 92 0.543
Positive 5 46
Medication use during pregnancy Negative 11 103 0.295
Medication use during pregnancy Positive 1 35
Threatened abortion Negative 10 126 0.310
Positive 2 12
Antepartum hemorrhage Negative 10 126 0.310
Positive 2 12
Maternal serum HBV DNA Negative 3 81 0.024* ,b
Positive 9 57
Maternal PBMC HBV rcDNA Negative 7 88 0.760
Positive 5 50
Maternal PBMC HBV cccDNA Negative 8 127 0.020*
Positive 4 11
Maternal HBeAg Negative 2 91 0.001*
Positive 10 47
Tab.1  Influencing factors of HBV intrauterine transmission
HBV intrauterine transmission OR (95% CI)
Yes ( n) No ( n)
rcDNA/cccDNA
− and − 6 87 1.0
+ or+ 6 51 1.344 (0.349−5.181)
− and+ 1 1 21.532 (0.891−520.227)
+ and − 2 40 0.564 (0.095−3.336)
+ and+ 3 10 3.586 (0.589−21.827)
rcDNA
7 88 1.0
+ 5 50 0.430 (0.082−2.238)
cccDNA
8 127 1.0
+ 4 11 6.003 (1.249−28.855)
Tab.2  Effect of maternal PBMC HBV infection and replication on HBV intrauterine transmission
β S.E. Wald c2 P OR (95% CI)
PBMC HBV rcDNA or cccDNA
HBeAg 1.374 0.358 14.745 <0.001 3.953 (1.96–7.973)
PBMC HBV rcDNA
HBeAg 1.360 0.359 14.334 <0.001 3.896 (1.929–7.876)
PBMC HBV cccDNA
HBeAg 1.319 0.586 5.070 <0.024 3.74 (1.186–11.793)
HBIG administration −1.165 0.570 4.168 0.041 0.312 (0.102–0.954)
Tab.3  Influencing factors of PBMC HBV infection and replication in HBsAg-positive mothers
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