Please wait a minute...
Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2008, Vol. 2 Issue (2) : 195-199    https://doi.org/10.1007/s11684-008-0037-7
Chemotactic effect of urokinase-type plasminogen activator on mouse spermatozoa
DING Xiaofang1, LI Honggang2, XIONG Chengliang2
1.Centre of Reproductive Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology; 2.Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology;
 Download: PDF(119 KB)   HTML
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract The aim of this study is to investigate the chemotactic effect of urokinase-type plasminogen activator (uPA) on mouse spermatozoa. Capillary assays were applied to study the chemotactic activity of ascending and descending gradients of uPA. Firstly, the chemotactic effect of an ascending gradient of uPA on mouse spermatozoa was observed by counting the number of spermatozoa that migrated into the capillary after incubation with uPA for 5, 10, 20, and 30 min, respectively, compared with that after incubation with F10. Twenty minutes was a suitable incubation time to obtain a plateau of sperm accumulation. Meanwhile, to confirm the specific effect of uPA on mouse sperm chemotaxis, uPA inhibitor (PAI-1) and anti-uPAR rabbit IgG were added to the test solution containing 20 U/mL uPA, respectively. To exclude the possibility that PAI-1 and anti-uPAR rabbit IgG may affect sperm accumulation nonspecifically, PAI-1 and anti-uPAR rabbit IgG were added to F10, respectively. It was found that the chemotactic effect of uPA was neutralized completely by PAI-1 and anti-uPAR rabbit IgG. PAI-1 and anti-uPAR rabbit IgG had no neutralizing effect on the sperm chemotactic effect. Lastly, the sperm chemotaxis response to a descending gradient of uPA was also observed. Taken together, the results suggest that uPA can induce sperm chemotaxis in vitro by binding to its receptor on the sperm membrane and may act as a chemoattractant in precontacting sperm-egg communication thereby increasing the chance encounter of spermatozoa and eggs.
Issue Date: 05 June 2008
 Cite this article:   
LI Honggang,DING Xiaofang,XIONG Chengliang. Chemotactic effect of urokinase-type plasminogen activator on mouse spermatozoa [J]. Front. Med., 2008, 2(2): 195-199.
 URL:  
https://academic.hep.com.cn/fmd/EN/10.1007/s11684-008-0037-7
https://academic.hep.com.cn/fmd/EN/Y2008/V2/I2/195
1 Cohen-Dayag A Tur-Kaspa I Dor J Mashiach S Eisenbach M Sperm capacitation in humans is transientand correlates with chemotactic responsiveness to follicular factorsProc Natl Acad Sci USA 1995 92(24)1103911043.
doi:10.1073/pnas.92.24.11039
2 Eisenbach M Giojalas L C Sperm guidance in mammals-anunpaved road to the eggMol Cell Biol 2006 7(4)276285
3 Bahat A Eisenbach M Sperm thermotaxisMol Cell Endocrinol 2006 252(1–2)115119.
doi:10.1016/j.mce.2006.03.027
4 Eisenbach M Mammaliansperm chemotaxis and its association with capacitationDev Genet 1999 25(2)8794.
doi:10.1002/(SICI)1520‐6408(1999)25:2<87::AID‐DVG2>3.0.CO;2‐4
5 Luconi M Forti G Baldi E Pathophysiology of sperm motilityFront Biosci 2006 1114331447.
doi:10.2741/1894
6 Sun F Giojalas L C Rovasio R A Tur-Kaspa I Sanchez R Eisenbach M Lack of species-specificity in mammalian sperm chemotaxisDev Biol 2003 255(2)423427.
doi:10.1016/S0012‐1606(02)00090‐8
7 Cao M Sahmi M Lussier J G Price C A Plasminogenactivator and serine protease inhibitor-E2 (protease nexin-1) expressionby bovine granulosa cells in vitroBiolReprod 2004 71(3)887893.
doi:10.1095/biolreprod.104.029702
8 Tsantarliotou M P Zervos I A Vatzias G Billinis C Taitzoglou I A Kokolis N A Plasminogen activator activity in the porcine oviduct duringthe oestrous cycleTheriogenology 2005 64(4)10071015.
doi:10.1016/j.theriogenology.2005.01.005
9 Nordengren J Pilka R Noskova V Ehinger A Domanski H Andersson C Høyer-Hansen G Hansson S R Casslén B Differentiallocalization and expression of urokinase plasminogen activator (uPA),its receptor (uPAR), and its inhibitor (PAI-1) mRNA and protein inendometrial tissue during the menstrual cycleMol Hum Reprod 2004 10(9)655663.
doi:10.1093/molehr/gah081
10 Zheng P Zou R J Liu Y X Source of plasminogen activator in rhesus monkey semenand its possible role in sperm capacitationActa Physiol Sin 2001 53(1)4550
11 Liu Y X Involvementof plasminogen activator and plasminogen activator inhibitor type1 in spermatogenesis, sperm capacitation, and fertilizationSemin Thromb Hemost 2007 33(1)2940.
doi:10.1055/s‐2006‐958459
12 Andreasen P A Kjoller L Christensen L Duffy M J The urokinase-typeplasminogen activator system in cancer metastasis: a reviewInt J Cancer 1997 72(1)122.
doi:10.1002/(SICI)1097‐0215(19970703)72:1<1::AID‐IJC1>3.0.CO;2‐Z
13 Sturge J Wienke D East L Jones G E Isacke C M GPI-anchored uPAR requires Endo180 forrapid directional sensing during chemotaxisJ Cell Biol 2003 162(5)789794.
doi:10.1083/jcb.200302124
14 Ding X F Xiong C L Effects of urokinase-type plasminogenactivator on chemotactic responses of spermatozoa in vitroZhonghua Nan Ke Xue Za Zhi 2005 11(6)409412(in Chinese)
15 Ming Y Shang X J Xiong C L Pang X B Xiong F Urokinase-type plasminogen activator improvesthe reproductive function of male ratsZhonghuaNan Ke Xue Za Zhi 2006 12(11)963967(in Chinese)
16 Biggers J D Whitten W K Whittingham D G The culture of mouse embryos in vitroIn: Methods in Mammalian Embryology Eds. Daniel J C, San FranciscoFreeman Press 1971 86116
17 Ralt D Manor M Cohen-Dayag A Tur-Kaspa I Ben-Shlomo I Makler A Yuli I Dor J Blumberg S Mashiach S Eisenbach M Chemotaxisand chemokinesis of human spermatozoa to follicular factorsBiol Reprod 1994 50(4)774785.
doi:10.1095/biolreprod50.4.774
18 Crippa M P Urokinase-typeplasminogen activatorInt J Biochem CellBiol 2007 39(4)690694.
doi:10.1016/j.biocel.2006.10.008
19 Sidenius N Blasi F The urokinase plasminogen activatorsystem in cancer: Recent advances and implication for prognosis andtherapyCancer Metastasis Rev 2003 22(2–3)205222.
doi:10.1023/A:1023099415940
20 Koyama S Amarie D Soini H A Novotny M V Jacobson S C Chemotaxis assays of mouse sperm on microfluidicdevicesAnal Chem 2006 78(10)33543359.
doi:10.1021/ac052087i
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed