Influence of β-elemene on the secretion of angiotensin II and expression of AT1R in hepatic stellate cells

doi:10.1007/s11684-009-0020-y

Front Med Chin

2009, Vol. 3

Issue (1) : 36-40 https://doi.org/10.1007/s11684-009-0020-y

RESEARCH ARTICLE

Influence of β-elemene on the secretion of angiotensin II and expression of AT1R in hepatic stellate cells

Ling YANG¹, Rui ZHU², Qingjing ZHU³, Dan DAN¹, Jin YE¹(

), Keshu XU¹, Xiaohua HOU¹

1. Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China; 2. Department of Traditional Chinese Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430022, China; 3. Department of Integrated Traditional Chinese Medicine and Western Medicine, Wuhan Municipal Hospital of Infectious Diseases, Wuhan 430022, China

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Abstract

This study aims to investigate the influence of β-elemene on the secretion of angiotensin II (ANG II) and the expression of angiotensin receptor type 1 (AT1R) in hepatic stellate cells (HSCs). In vitro, HSC-T6 were cultured for 24 hours and then treated with different doses of β-elemene (2.5, 5 and 10 mg/L). A control group was also set up. The secretion of ANG II in the supernatant was detected by radioimmunoassay. The mRNA expression of AT1R at 4, 12 and 24 h after treatment was detected by reverse transcription-polymerase chain reaction (RT-PCR), respectively. The protein expression of AT1R was detected by western blot. At the 4th h, the ANG II secretion in the supernatant was significantly inhibited by 10 mg/L β-elemene compared with the control group (P<0.05), while 5.0 mg/L and 2.5 mg/L β-elemene had no inhibitory effect on the secretion of ANG II (P>0.05). At the time point of the 12th h, the secretion of ANG II in the supernatant treated with 10 mg/L and 5.0 mg/L β-elemene was significantly lower than the control (P<0.01, P<0.05). Following the treatment with 5.0 mg/L and 2.5 mg/L β-elemene for 24 h, significant inhibition of ANG II secretion was observed (P<0.05), but 10 mg/L β-elemene had no such effect. β-elemene significantly reduced the amount of AT1R mRNA in HSCs after the treatment for 4, 12, and 24 h in a dose-dependent manner. The expression of AT1R protein also decreased after the treatment with β-elemene for 24 h. β-elemene can inhibit the secretion of ANG II and the gene and protein expression of AT1R, which may be the mechanism by which β-elemene prevents the progress of hepatic fibrosis.

Keywords liver cirrhosis beta-elemene hepatic stellate cells angiotensin II receptor, angiotensin, type 1

Corresponding Author(s): YE Jin,Email:yejin8689@sina.com

Issue Date: 05 March 2009

Cite this article:

Ling YANG,Rui ZHU,Qingjing ZHU, et al. Influence of β-elemene on the secretion of angiotensin II and expression of AT1R in hepatic stellate cells[J]. Front Med Chin, 2009, 3(1): 36-40.

URL:

https://academic.hep.com.cn/fmd/EN/10.1007/s11684-009-0020-y
https://academic.hep.com.cn/fmd/EN/Y2009/V3/I1/36

Tab.1 Inhibitory effect of β-elemene on the secretion of ANG II in HSCs
pmol·L

Fig.1 β-elemene inhibited the expression of AT1R mRNA in hepatic stellate cells. (a) HSC-T6 were incubated in DMEM with 10% FBS. After incubation for 24 h, β-elemene of the indicated concentration was added to the cells and incubation was continued for the indicated time. AT1R mRNA was determined by RT-PCR. (b) AT1R protein in HSCs following treatment with 10, 5.0 and 2.5 mg/L β-elemene for 24 h was detected by western blot. Values are presented as ( = 3 for each experimental group). Three independent experiments were performed (: <0.001 control cells). Lane 1: 10 mg/L β-elemene; Lane 2: 5.0 mg/L β-elemene; Lane 3: 2.5 mg/L β-elemene.

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