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Effects of resistin on insulin signaling in endothelial cells |
Zhizhen LI1, Fangping LI2, Jianhong YE2, Li YAN2, Zuzhi FU2() |
1. Department of Endocrinology, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China; 2. Department of Endocrinology, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China |
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Abstract The objective of this study was to investigate the effects of resistin on insulin signaling in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with recombinant human resistin (0-100 ng/mL) for 24 h. Akt and endothelial nitric oxide synthase (eNOS) phosphorylation levels of endothelial cells under basal or insulin stimulated conditions were measured by Western blot. Nitric oxide (NO) production of HUVECs was also detected. The results showed that resistin could significantly inhibit Akt and eNOS phosphorylation and NO production in endothelial cells under insulin stimulated conditions (P< 0.05 vs control). But under basal conditions, treatment with resistin could result in a decrease in eNOS phosphorylation (P< 0.05 vs control) but had no effect on NO production and Akt phosphorylation levels. These findings suggested that resistin exerted an inhibitory effect on NO production by inhibiting insulin signaling and eNOS phosphorylation in endothelial cells.
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Keywords
resistin
endothelium
nitric oxide
endothelial nitric oxide synthase
Akt-binding protein, mouse
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Corresponding Author(s):
FU Zuzhi,Email:lzz940537@163.com
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Issue Date: 05 June 2009
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