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Identification of Legionella species by the composition of cellular fatty acids |
Cun-Lei FANG MD1,Zhao-Hui HU PhD1,Qing-Yi ZHU MD1,Ya-Jun SONG PhD2,Ya-Fang TAN PhD2,Rui-Fu YANG PhD2, |
1.Guangzhou Kingmed Center
for Clinical Laboratory, Guangzhou 510330, China; 2.Institute of Microbiology
and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing
100071, China; |
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Abstract One hundred and fifty-three isolates from the environment and 36 reference strains of the Legionella were studied with regards to their composition of cellular fatty acids as determined by gas chromatography, and then were classified into 41 groups by numerical analysis. Most reference strains formed only a single group, except L. micdadei, L. jamestowniensis, L. parisiensis, L. jordanis, L. feeleii and L. longbeachae, which were clustered into two or three groups. Even serological types of L. pneumophila could be clearly identified. Therefore, in this study, numerical analysis of cellular fatty acid composition is an effective method for identifying Legionella species.
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Keywords
Legionella
numerical analysis
identification
fatty acids composition
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Issue Date: 05 June 2010
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