Please wait a minute...
Frontiers of Medicine

ISSN 2095-0217

ISSN 2095-0225(Online)

CN 11-5983/R

Postal Subscription Code 80-967

2018 Impact Factor: 1.847

Front. Med.    2010, Vol. 4 Issue (2) : 208-215    https://doi.org/10.1007/s11684-010-0031-8
Research articles
Identification of Legionella species by the composition of cellular fatty acids
Cun-Lei FANG MD1,Zhao-Hui HU PhD1,Qing-Yi ZHU MD1,Ya-Jun SONG PhD2,Ya-Fang TAN PhD2,Rui-Fu YANG PhD2,
1.Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, China; 2.Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China;
 Download: PDF(203 KB)  
 Export: BibTeX | EndNote | Reference Manager | ProCite | RefWorks
Abstract One hundred and fifty-three isolates from the environment and 36 reference strains of the Legionella were studied with regards to their composition of cellular fatty acids as determined by gas chromatography, and then were classified into 41 groups by numerical analysis. Most reference strains formed only a single group, except L. micdadei, L. jamestowniensis, L. parisiensis, L. jordanis, L. feeleii and L. longbeachae, which were clustered into two or three groups. Even serological types of L. pneumophila could be clearly identified. Therefore, in this study, numerical analysis of cellular fatty acid composition is an effective method for identifying Legionella species.
Keywords Legionella      numerical analysis      identification      fatty acids composition      
Issue Date: 05 June 2010
 Cite this article:   
Cun-Lei FANG MD,Qing-Yi ZHU MD,Zhao-Hui HU PhD, et al. Identification of Legionella species by the composition of cellular fatty acids[J]. Front. Med., 2010, 4(2): 208-215.
 URL:  
https://academic.hep.com.cn/fmd/EN/10.1007/s11684-010-0031-8
https://academic.hep.com.cn/fmd/EN/Y2010/V4/I2/208
Yu V L, Plouffe J F, Pastoris M C. Distribution of Legionella species and serogroups isolatedby culture in patients with sporadic community-acquired legionellosis:an international collaborative study. J Infect Dis, 2002, 186(1): 127–128

doi: 10.1086/341087
Brenner D J. Classification of Legionellaceae. Current status and remaining questions. Isr J Med Sci, 1986, 22(9): 620–632
Grimont F, Lefèvre M, Ageron E, Grimont P A. rRNA gene restriction patterns of Legionella species: a molecular identification system. Res Microbiol, 1989, 140(8): 615–626

doi: 10.1016/0923-2508(89)90002-8
Ratcliff R M, Lanser J A, Manning P A, Heuzenroeder M W. Sequence-based classification schemefor the genus Legionella targeting the mip gene. J Clin Microbiol, 1998, 36(6): 1560–1567
Hookey J V, Birtles R J, Saunders N A. Intergenic 16S rRNA gene(rDNA)-23S rDNA sequence length polymorphisms in members of the family Legionellaceae. J Clin Microbiol, 1995, 33(9): 2377–2381
Riffard S, Lo Presti F, Normand P, Forey F, Reyrolle M, Etienne J, Vandenesch F. Species identification of Legionella via intergenic 16S–23S ribosomal spacerPCR analysis. Int J Syst Bacteriol, 1998, 48(3): 723–730

doi: 10.1099/00207713-48-3-723
Veríssimo A, Morais P V, Diogo A, Gomes C, da Costa M S. Characterization of Legionella species by numerical analysis of whole-cell protein electrophoresis. Int J Syst Bacteriol, 1996, 46(1): 41–49

doi: 10.1099/00207713-46-1-41
Devos L, Clymans K, Boon N, Verstraete W. Evaluation of nested PCR assays for the detection of Legionella pneumophila in a wide range ofaquatic samples. J Appl Microbiol, 2005, 99(4): 916–925

doi: 10.1111/j.1365-2672.2005.02668.x
Joly P, Falconnet P A, Andre J, Weill N, Reyrolle M. Quantitative real-time Legionella PCR for environmentalwater samples: data interpretation. Appl Environ Microbiol, 2006, 72(4): 2801–2808

doi: 10.1128/AEM.72.4.2801-2808.2006
O'Donnell A G. Numerical analysis of chemotaxonomic data. Computer-assisted bacterial systematics. London: Academic Press, Inc. (London), Ltd., 1985, 403–414
O'Donnell A G, Nahaie M R, Goodfellow M, Minnikin D E, Hajek V. Numerical analysisof fatty acid profiles in the identification of staphylococci. J Gen Microbiol, 1985, 131(8): 2023–2033
Dees S B, Powell J, Moss C V, Hollis D G, Weaver R E. Cellular fatty acid composition of organisms frequently associatedwith human infections resulting from dog bites: Pasteurella multocida and groups EF-4, IIj, M-5, and DF-2. J Clin Microbiol, 1981, 14(6): 612–616
Miller L, Berger T. Bacteria identification by gas chromatography of whole cell fatty acids. Hewlett-Packard Application Note. Avondale, PA: Hewlett-Packard Co., 1985, 228–241
Steele M, McNab W B, Read S, Poppe C, Harris L, Lammerding A M, Odumeru J A. Analysis of whole-cell fatty acid profiles of verotoxigenic Escherichia coli and Salmonella enteritidis with the microbial identification system. Appl Environ Microbiol, 1997, 63(2): 757–760
Larsson L, Mårdh P A. Gas chromatographic characterization of mycobacteria: analysis of fatty acids and trifluoroacetylated whole-cell methanolysates. J Clin Microbiol, 1976, 3(2): 81–85
Janse J D, Smit P H. Whole cell fatty acid patterns of Xenorhabdus species. Lett Appl Microbiol, 1990, 10(3): 131–135

doi: 10.1111/j.1472-765X.1990.tb00099.x
Teska J D, Coyne S R, Ezzell J W, Allan C M, Redus S L. Identification of Bacillus anthracis using gas chromato-graphic analysis of cellular fatty acids and a commercially available database. Agilent Technologies, Inc. 2003, 1–5
Lambert M A, Patton C M, Barrett T J, Moss C W. Differentiation of Campylobacter and Campylobacter-like organisms by cellular fatty acid composition. J Clin Microbiol, 1987, 25(4): 706–713
Sasser M. Identification of bacteria by gas chromatography of cellularfatty acids. MIDI Inc. Technical Note #101, 1990, 1–7
Lambert M A, Moss C W. Cellular fatty acid compositions and isoprenoid quinone contents of 23 Legionella species. J Clin Microbiol, 1989, 27(3): 465–473
Diogo A, Verissimo A, Nobre M F, Da Costa M S. Usefulness of fatty acid composition for differentiationof Legionella species. J Clin Microbiol, 1999, 37(7): 2248–2254
Birtles R J, Rowbotham T J, Raoult D, Harrison T G. Phylogenetic diversity of intra-amoebal Legionellae as revealed by 16S rRNA genesequence comparison. Microbiol, 1996, 142(12): 3525–3530

doi: 10.1099/13500872-142-12-3525
Hookey J V, Saunders N A, Fry N K, Birtles R J, Harrison T G. Phylogeny of legionellaceae based on small-subunit ribosomal dna sequences and proposal of legionellalytica comb nov for legionella-like amoebal pathogens. Int J Syst Bacteriol, 1996, 46(2): 526–531

doi: 10.1099/00207713-46-2-526
[1] Won-Mo Jung, In-Soo Park, Ye-Seul Lee, Chang-Eop Kim, Hyangsook Lee, Dae-Hyun Hahm, Hi-Joon Park, Bo-Hyoung Jang, Younbyoung Chae. Characterization of hidden rules linking symptoms and selection of acupoint using an artificial neural network model[J]. Front. Med., 2019, 13(1): 112-120.
[2] Xiao-Yong ZHAN MD, Chao-Hui HU PhD, Qing-Yi ZHU MD, . Research advances of Legionella and Legionnaires’ disease[J]. Front. Med., 2010, 4(2): 166-176.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed