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Design of tandem genes cluster for isoflavone
engineering |
| Xunli XIA PhD , Guangxiao YANG PhD , Guangyuan HE PhD , |
| China-UK HUST-RRes Genetics
Engineering and Genomics Joint Laboratory, Genetic Engineering International
Cooperation Base of the Ministry of Science and Technology of China,
Key Laboratory of Molecular Biophysics of Chinese Ministry of Education,
College of Life Science and Technology, Huazhong University of Science
and Technology, Wuhan 430074, China; |
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Abstract In this paper, a universal effective novel method of constructing tandem genes cluster for isoflavone engineering was reported. A tandem genes cluster CHS-CHI-IFS (rIFS) of secondary metabolites of plant isoflavones was constructed by using the chalcone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS) (GenBank accession numbers EU526827, EU526829, EU526830) in only one recombination with the pET22b vector. The resulting expression vector pET-rIFS was heterogeneously expressed and co-incubated with barrenwort extractions, and the genistein-like component was detected.
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| Keywords
isoflavones engineering
secondary metabolism
tandem genes cluster
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Issue Date: 05 September 2009
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