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Analysis of Brucella cellular fatty acids |
| Zhen-Xiang ZHAO MS1,Hai JIANG MD2,Bu-Yun CUI MS2,Hong-Yan ZHAO2,Dong-Ri PIAO MS2,Lan-Yu LI2,Su-Zhen HAO MS3, |
| 1.Department of Microbiology
and Immunology, Shanxi Medical University, Taiyuan 030001, China；National Institute for
Communicable Disease Control and Prevention, Chinese Center for Disease
Control and Prevention, Beijing 102206, China; 2.National Institute for
Communicable Disease Control and Prevention, Chinese Center for Disease
Control and Prevention, Beijing 102206, China; 3.Department of Microbiology
and Immunology, Shanxi Medical University, Taiyuan 030001, China; |
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Abstract To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included B. suis (bv. 1, 2, 3, 5) and B. ovis; cluster 2 included B. abortus (bv. 3, 4, 5, 6) and B. melitensis (bv. 1, 2, 3); cluster 3 included B. abortus (bv. 1, 2, 7, 9) and B. neotomae; cluster 4 was B. suis (bv. 4); and cluster 5 was B. canis. Typing Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of typing and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify B. suis (bv. 4) and B. canis.
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| Keywords
Brucella
gas chromatography
cellular fatty acids
dendrogram
typing
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Issue Date: 05 June 2010
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