Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality

doi:10.1007/s11684-017-0519-6

Front. Med.

2017, Vol. 11

Issue (2) : 247-252 https://doi.org/10.1007/s11684-017-0519-6

RESEARCH ARTICLE

Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality

Bo Huang, Zhou Li, Xinling Ren, Jihui Ai, Lixia Zhu(

), Lei Jin(

)

Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China

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Abstract

The activity of free radicals in follicular fluid was related to ovarian responsiveness, in vitro fertilization (IVF), and embryo transfer success rate. However, studies analyzing the relationship between the free radical scavenging capacity and embryo quality of infertile women with polycystic ovarian syndrome (PCOS) were lacking. The aim of this study was to evaluate the relationship between the free radical scavenging window of women with PCOS and their embryo quality. The free radical scavenging capacity of follicular fluid from women with PCOS was determined by a,a-diphenyl-b-picrylhydrazyl (DPPH), 2,2-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) assay, superoxide radical, and reactive oxygen species (ROS) assay. In the DPPH and ROS assays, the follicular fluid from grades I and II embryos was significantly higher than the follicular fluid from grades III and IV embryos. The lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level were 13.2% and 109.0 cps, respectively. The calculated lower control limit and upper control limit were further confirmed in the follicular fluid of embryos of all grades. These cut-off values of free radical scavenging activity of follicular fluid could assist embryologists in choosing the development of embryos in PCOS patients undergoing IVF.

Keywords in vitro fertilization PCOS free radical embryo quality

Corresponding Author(s): Lixia Zhu,Lei Jin

Just Accepted Date: 14 April 2017 Online First Date: 08 May 2017 Issue Date: 01 June 2017

Cite this article:

Bo Huang,Zhou Li,Xinling Ren, et al. Free radical scavenging window of infertile patients with polycystic ovary syndrome: correlation with embryo quality[J]. Front. Med., 2017, 11(2): 247-252.

URL:

https://academic.hep.com.cn/fmd/EN/10.1007/s11684-017-0519-6
https://academic.hep.com.cn/fmd/EN/Y2017/V11/I2/247

Fig.1 The free radical scavenging capacity of infertile women with PCOS: the correlation with embryo quality. (A) DPPH radical scavenging assay; (B) ABTS radical scavenging assay; (C) ROS levels assay. Grades I and II group, n=294; Grades III and IV group, n=108. Different letters differed significantly ((A) P = 0.045, (C) P =0.002).

Tab.1 The correlation between superoxide radical scavenging capacity in follicular fluid of infertile women undergoing IVF and their embryo quality

Fig.2 The control chart of lower control limit of DPPH radical scavenging capacity and upper control limit of ROS level in follicular fluid from women with PCOS. (A) lower control limit of DPPH radical scavenging capacity; (B) upper control limit of ROS level.

Tab.2 Identification of the correlation between cut-off level in radical scavenging capacity of follicular fluid from infertile women and their embryo quality

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