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Impact of HBV replication in peripheral blood mononuclear cell on HBV intrauterine transmission |
Xiaohong Shi1, Xuefei Wang1, Xixi Xu1, Yongliang Feng1, Shuzhen Li1, Shuying Feng2, Bo Wang2, Suping Wang1() |
1. Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China 2. Department of Obstetrics and Gynaecology, The Third People Hospital of Taiyuan City, Taiyuan 030001, China |
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Abstract This study determined the effect of hepatitis B virus (HBV) replication in peripheral blood mononuclear cell (PBMC) from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg-positive mothers and their neonates were recruited in this study. Within 24 h after birth, HBV serological markers, serum HBV DNA, PBMC HBV relaxed circular DNA (rcDNA), and covalently closed circular DNA (cccDNA) were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis. The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chi-square test and logistic regression. The rate of HBV intrauterine transmission was 8.00% (12/150) in the 150 neonates born to HBsAg-positive mothers. The positivities of PBMC HBV rcDNA and cccDNA in the HBsAg-positive mothers were 36.67% (55/150) and 10% (15/150), respectively. Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission (OR= 6.003, 95% CI: 1.249–28.855). Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA (OR= 3.896, 95% CI: 1.929–7.876) and PBMC HBV cccDNA (OR= 3.74, 95% CI: 1.186–11.793) in the HBsAg-positive mothers. Administration of hepatitis B immune globulin was a protective factor of PBMC HBV cccDNA (OR= 0.312, 95% CI: 0.102–0.954) during pregnancy. The positivity of PBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers (c2=5.087, P= 0.024). This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.
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Keywords
PBMC
HBV cccDNA
HBV rcDNA
HBV intrauterine transmission
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Corresponding Author(s):
Suping Wang
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Just Accepted Date: 30 October 2017
Online First Date: 22 November 2017
Issue Date: 04 December 2017
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