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Resveratrol reduces intracellular reactive oxygen species levels by inducing autophagy through the AMPK-mTOR pathway |
Jun Song1,2,3, Yeping Huang1, Wenjian Zheng4, Jing Yan1, Min Cheng5, Ruxing Zhao2, Li Chen2, Cheng Hu1(), Weiping Jia1() |
1. Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Key Clinical Center for Metabolic Diseases, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, China 2. Department of Endocrinology, Qilu Hospital of Shandong University, Jinan 250012, China 3. Department of Endocrinology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, China 4. Department of Geriatrics, Qingdao Haici Medical Treatment Group, Qingdao 266000, China 5. Huangdao Disease Prevention and Control Center, Qingdao 266555, China |
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Abstract Oxidative stress induced by free fatty acid aggravates endothelial injury, which leads to diabetic cardiovascular complications. Reduction of intracellular oxidative stress may attenuate these pathogenic processes. The dietary polyphenol resveratrol reportedly exerts potential protective effects against endothelial injury. This study determined whether resveratrol can reduce the palmitic acid (PA)-induced generation of reactive oxygen species (ROS) and further explored the underlying molecular mechanisms. We found that resveratrol significantly reduced the PA-induced endothelial ROS levels in human aortic endothelial cells. Resveratrol also induced endothelial cell autophagy, which mediated the effect of resveratrol on ROS reduction. Resveratrol stimulated autophagy via the AMP-activated protein kinase (AMPK)-mTOR pathway. Taken together, these data suggest that resveratrol prevents PA-induced intracellular ROS by autophagy regulation via the AMPK-mTOR pathway. Thus, the induction of autophagy by resveratrol may provide a novel therapeutic candidate for cardioprotection in metabolic syndrome.
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Keywords
resveratrol
reactive oxygen species
AMPK
mTOR
autophagy
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Corresponding Author(s):
Cheng Hu,Weiping Jia
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Just Accepted Date: 12 September 2018
Online First Date: 13 November 2018
Issue Date: 03 December 2018
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